LOCUS       sequence-379414-        4086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A7_STOP
FEATURES             Location/Qualifiers
     source          1..4086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1643..1742)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1759..1778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1760..1778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1783..1799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1783..1799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1901..1920)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1912..2721
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1979..1998)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2868..3456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3357..3376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3610..3627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3786..3813)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3905..3991)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4040..4062
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4054..4071
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4055..4071
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt ggactccacc
      121 gagtacgagg tggcctctca gccagaggtg gagacaagcc cactgggcga tggagcatcc
      181 ccaggacctg agcaggtgaa gctgaagaag gagatcagcc tgctgaacgg agtatgcctc
      241 atagtgggta atatgatcgg ctctggcatc tttgtgagcc ccaagggcgt gctgatctac
      301 tctgccagct tcggcctgag cctggtcatc tgggcagtgg gcggcctgtt ctccgtgttt
      361 ggcgccctgt gctatgcaga gctgggaacc acaatcaaga agtctggcgc cagctacgcc
      421 tatatcctgg aggccttcgg cggctttctg gccttcatcc ggctgtggac atctctgctg
      481 atcatcgagc caaccagcca ggccatcatc gccatcacat tcgccaacta catggtgcag
      541 cccctgtttc cttcctgctt cgccccctat gcagcatcta gactgctggc agcagcatgc
      601 atctgtctgc tgacctttat caattgtgcc tacgtgaagt ggggcacact ggtgcaggac
      661 atcttcacct atgccaaggt gctggccctg atcgccgtga tcgtggcagg aatcgtgcgg
      721 ctgggacagg gagcatctac acactttgag aacagcttcg agggcagctc ctttgccgtg
      781 ggcgacatcg ccctggccct gtacagcgcc ctgttctcct attctggctg ggataccctg
      841 aattacgtga cagaggagat caagaaccca gagaggaatc tgcccctgtc catcggcatc
      901 tctatgccta tcgtgaccat catctatatc ctgacaaacg tggcctacta taccgtgctg
      961 gacatgaggg atatcctggc atccgacgca gtggcagtga cctttgccga tcagatcttt
     1021 ggcatcttca actggatcat ccctctgagc gtggccctgt cctgcttcgg aggcctgaat
     1081 gcatccatcg tggcagcctc taggctgttc tttgtgggaa gcagggaggg acacctgcca
     1141 gacgccatct gcatgatcca cgtggagagg tttacaccag tgccctccct gctgttcaac
     1201 ggcatcatgg ccctgatcta cctgtgcgtg gaggatatct ttcagctgat caattactat
     1261 agcttctcct actggttctt tgtgggcctg tccatcgtgg gccagctgta tctgaggtgg
     1321 aaggagcctg acaggcctag gccactgaag ctgagcgtgt tcttcccaat cgtgttttgc
     1381 ctgtgcacca tcttcctggt ggccgtgccc ctgtacagcg atacaatcaa ctccctgatc
     1441 ggaatcgcaa tcgccctgtc tggactgcct ttctattttc tgatcatccg ggtgcctgag
     1501 cacaagagac cactgtacct gcggagaatc gtgggctctg ccacccggta tctgcaggtg
     1561 ctgtgcatga gcgtggcagc agagatggac ctggaggatg gaggcgagat gcccaagcag
     1621 agagatccta agtccaatta agacccagct ttcttgtaca aagttggcat tataagaaag
     1681 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1741 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1801 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1861 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1921 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1981 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2041 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2101 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2161 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2221 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2281 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2341 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2401 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2461 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2521 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2581 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2641 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2701 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2761 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3721 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3781 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3841 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3901 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3961 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4021 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4081 gggccc
//
