LOCUS       sequence-379417-        6673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6673)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..6673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     954..973
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1148..1171
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3401..3522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3438..3457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3492..3511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3529..3984)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3666..3687)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3878..3897
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4011..4115
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4117..4474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4117..4308
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4140..4160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4325..4460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4509..5303
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4509..5303
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4563..4582)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5173..5192
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5491..5510)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5535..5582
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5911..6499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6400..6419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtggcta ccatggtgag caagggcgag gaggtcatca aggagttcat gcgcttcaag
      601 gagcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggcaccca gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc
      721 ttcgcctggg acatcctgtc ccctcagatc atgtacggct ccaaggccta cgtgaagcac
      781 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      841 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      901 ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact tcccctccga cggccccgta
      961 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1021 gccctgaagg gcgagatgaa gatgaggctg aggctgaagg acggcggcca ctacgacgcc
     1081 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc
     1141 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     1201 cgcgccgagg gccgccactc caccggcgcc tccggactca gatctcgagt gagcgctctg
     1261 gcctcccgcg ctaccgccat gcagtcctcc ggctccttca actacgcgcg ccctaagcag
     1321 ttcatcgcgg cgcagaacct gggtcccgca tcggggctgc ccacgccaac ctccagcccc
     1381 agctcctcca gcttgccgtc accattgtcc cccacgccca ggcccttcgg ccgcgcgccc
     1441 gggccgccct tcgtggaacc cgaggccatg tggggcccgt cctcgccctc gccgccaccg
     1501 ccgccacctc cggtcttcag cccctctgca gcctacccgg tgcccgacgt gttcccactg
     1561 ccaccgccgc cgccaccgct acctagctcc acctcacact gcgcctcgcc cgcccgcttc
     1621 ggccccagcc agacgcccgc agccttcctc agcgccctgc tgccctctca gccaccgcct
     1681 gtcgctgtca acgccctggg gctgcccaag ggagtcaccc ccgcggggtt cccaaagaag
     1741 tccagtagaa ctgctagaat tgcctctgat gaggagattc aaggcacaaa ggatgctgtc
     1801 atccaagacc tggaacggaa gcttcgcttc aaggaggacc ttctgaacaa tggccaaccg
     1861 aggctaacct atgaggaaag aatggctcgc cgcctgcttg gagccgacag cgcaaacgtc
     1921 ttcaacatcc aggagccaga ggaaacggca gccaatcagg agtacaaagt ctctagctgc
     1981 gagcagaggc tgattagcga gattgaatac aggctggagc gctcccctgt ggatgagtcg
     2041 ggagacgagg tgcaggatcc agatgtgcct gtggagaacg caacagctcc cttctttgag
     2101 atgaagctga aacactacaa gatctttgag gggatgccgg tgactttcac gtgtcgagtg
     2161 gctgggaatc caaagccaaa gatctattgg tttaaagatg ggaagcagat ttctccgaag
     2221 agcgatcact acaccattca gagagacctt gatgggacct gctctctcca caccacggcc
     2281 tctaccctag acgacgatgg gaactacacc atcatggctg ccaaccctca gggtcgcgtc
     2341 agttgtacag gaaggctaat ggtacaggct gtcaaccaaa gaggccgcag tccccgctct
     2401 ccctcaggcc atcctcatgc cagaaggcct cgctctcgat cacgggacag tggagatgaa
     2461 aacgagccca ttcaggagcg attcttcaga cctcacttcc tgcaggctcc tggagacctg
     2521 accgttcagg aaggcaagct ctgcaggatg gactgcaagg tcagtgggtt accaacccca
     2581 gatctcagct ggcaactaga cggaaagccc atacgccccg acagtgctca caagatgctg
     2641 gtccgtgaga atggggtcca ctccctcatt atagagccag tcacgtcccg ggacgccggc
     2701 atctacacat gtattgccac caacagagca ggacagaact cgtttaacct ggagcttgtg
     2761 gttgctgcta aggaagcaca caaggcccct gtgtttatgg agaagctaca gaacacgggg
     2821 gttgctgatg gatacccagt gcggctagaa tgccgtgtct ccggagtgcc gccacctcag
     2881 atattttgga agaaagaaaa tgaatcgctc actcatagca ctgagcgagt aagcatgcac
     2941 caggataatc atggctacat ctgcctgctc atccagggag ccacaaagga agacgctggg
     3001 tggtacactg tgtctgccaa gaacgaagca ggcattgtgt cctgcactgc ccggctggat
     3061 gtctacaccc agtggcacca gcagccacag accaccaagc caaaaaaagt acggccctcg
     3121 gccagtcgct acgcagcact ttcggaccag ggactagaca tcaaagccgc tttccaacct
     3181 gaagccagcc catctcacct gactctgaac agcggcttgg tagaaagtga agacctgtga
     3241 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     3301 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3361 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3481 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     3541 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3601 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3661 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3721 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3781 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3841 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3901 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3961 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4021 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4081 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     4141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4381 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4441 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4501 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4561 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4621 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4681 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4741 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4801 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4861 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4921 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4981 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     5041 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5101 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5161 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5221 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5281 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5341 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5401 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5461 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5521 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5581 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5641 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5701 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6601 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     6661 atatggagtt ccg
//
