LOCUS       sequence-379420-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A8_STOP
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1715..1814)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1831..1850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1832..1850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1855..1871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1855..1871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1973..1992)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1984..2793
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2051..2070)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2940..3528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3429..3448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3682..3699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3858..3885)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3977..4063)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4112..4134
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4126..4143
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4127..4143
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ggagggcgcc
      121 cggcacagaa acaataccga gaagaagcac ccaggcggcg gcgagtccga cgcctctcca
      181 gaggcaggat ctggaggagg aggagtggcc ctgaagaagg agatcggcct ggtgagcgcc
      241 tgcggcatca tcgtgggcaa catcatcggc tccggcatct tcgtgtctcc caagggcgtg
      301 ctggagaatg caggaagcgt gggactggcc ctgatcgtgt ggatcgtgac cggctttatc
      361 acagtggtgg gcgccctgtg ctacgcagag ctgggcgtga ccatcccaaa gagcggcggc
      421 gactactcct atgtgaagga tatcttcgga ggactggcag gctttctgag gctgtggatc
      481 gccgtgctgg tcatctaccc caccaaccag gccgtgatcg ccctgacatt ctccaattat
      541 gtgctgcagc ctctgttccc aacctgcttt ccccctgagt ctggcctgag actgctggcc
      601 gccatctgcc tgctgctgct gacatgggtg aactgtagct ccgtgaggtg ggcaaccagg
      661 gtgcaggata tcttcacagc aggcaagctg ctggccctgg cactgataat tataatgggc
      721 attgtccaga tctgtaaggg cgagtacttt tggctggagc ctaagaacgc cttcgagaat
      781 tttcaggagc cagacatcgg actggtggcc ctggccttcc tgcagggctc ttttgcctac
      841 ggcggctgga acttcctgaa ttatgtgacc gaggagctgg tggatcccta taagaacctg
      901 cctagggcca tctttatctc catccctctg gtgaccttcg tgtacgtgtt tgccaacgtg
      961 gcctatgtga cagccatgtc tccacaggag ctgctggcca gcaatgccgt ggccgtgaca
     1021 ttcggcgaga agctgctggg cgtgatggcc tggatcatgc ctatctctgt ggccctgagc
     1081 accttcggcg gcgtgaatgg cagcctgttt acatctagcc ggctgttctt tgcaggagca
     1141 agagagggac acctgccatc cgtgctggcc atgatccacg tgaagcggtg caccccaatc
     1201 cccgccctgc tgtttacctg tatctccaca ctgctgatgc tggtgacctc tgacatgtac
     1261 acactgatca actatgtggg cttcatcaat tacctgtttt atggcgtgac agtggccggc
     1321 cagatcgtgc tgaggtggaa gaagccagat atccctcgcc caatcaagat caacctgctg
     1381 ttccccatca tctacctgct gttctgggcc tttctgctgg tgtttagcct gtggtccgag
     1441 cctgtggtgt gcggaatcgg actggcaatc atgctgaccg gagtgcccgt gtacttcctg
     1501 ggcgtgtatt ggcagcacaa gcctaagtgc ttcagcgact ttatcgagct gctgaccctg
     1561 gtgtcccaga agatgtgcgt ggtggtgtac ccagaggtgg agaggggatc cggcacagag
     1621 gaggccaatg aggatatgga ggagcagcag cagcctatgt atcagcccac ccctacaaag
     1681 gacaaggatg tggccggcca gccacagccc taagacccag ctttcttgta caaagttggc
     1741 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1801 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1861 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1921 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1981 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2041 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2101 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2161 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2221 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2281 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2341 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2401 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2461 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2521 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2581 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2641 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2701 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2761 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2821 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaagc tcgggccc
//
