LOCUS       sequence-379422-        4014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A9_STOP
FEATURES             Location/Qualifiers
     source          1..4014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1571..1670)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1687..1706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1688..1706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1711..1727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1711..1727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1829..1848)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1840..2649
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1907..1926)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2796..3384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3285..3304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3538..3555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3714..3741)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3833..3919)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3968..3990
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3982..3999
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3983..3999
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg cgacaccgga
      121 ctgaggaaga ggagagagga tgagaagtcc atccagtctc aggagccaaa gaccacatcc
      181 ctgcagaagg agctgggcct gatcagcggc atctccatca tcgtgggcac catcatcggc
      241 agcggcatct tcgtgagccc aaagagcgtg ctgtccaata cagaggcagt gggaccatgc
      301 ctgatcatct gggcagcatg tggcgtgctg gccaccctgg gcgccctgtg cttcgccgag
      361 ctgggcacca tgatcacaaa gtccggcggc gagtacccct atctgatgga ggcctacggc
      421 cctatcccag cctatctgtt ttcttgggcc agcctgatcg tgatcaagcc tacaagcttt
      481 gccatcatct gcctgagctt ctccgagtac gtgtgcgccc cattctatgt gggctgtaag
      541 ccccctcaga tcgtggtgaa gtgtctggcc gccgccgcca tcctgtttat ctctaccgtg
      601 aattctctga gcgtgagact gggcagctac gtgcagaaca tcttcacagc cgccaagctg
      661 gtcatcgtgg ccatcatcat catcagcgga ctggtgctgc tggcacaggg caacaccaag
      721 aattttgaca actccttcga gggagcacag ctgagcgtgg gagccatctc cctggccttt
      781 tacaatggcc tgtgggccta tgatggctgg aatcagctga actacatcac agaggagctg
      841 aggaatccat atcgcaacct gcccctggcc atcatcatcg gcatccctct ggtgaccgcc
      901 tgctacatcc tgatgaacgt gtcctacttc accgtgatga ccgccacaga gctgctgcag
      961 tctcaggcag tggcagtgac cttcggcgac agggtgctgt acccagcatc ctggatcgtg
     1021 cctctgtttg tggccttctc tacaatcggc gccgccaacg gcacctgttt tacagcaggc
     1081 cggctgatct acgtggcagg aagagagggc cacatgctga aggtgctgtc ctatatctct
     1141 gtgaggcgcc tgaccccagc acctgccatc atcttctacg gcatcatcgc cacaatctat
     1201 atcatccccg gcgatatcaa ttctctggtg aactacttca gctttgccgc ctggctgttt
     1261 tatggcctga ccatcctggg cctgatcgtg atgcggttca caagaaagga gctggagcgc
     1321 ccaatcaagg tgcccgtggt catccctgtg ctgatgaccc tgatcagcgt gttcctggtg
     1381 ctggccccta tcatctccaa gccaacatgg gagtacctgt attgcgtgct gttcatcctg
     1441 tctggcctgc tgttctactt tctgttcgtg cactataagt tcggctgggc ccagaagatc
     1501 agcaagccca tcaccatgca cctgcagatg ctgatggagg tggtgccacc cgaggaggac
     1561 cctgagtaag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1621 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1681 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1741 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1801 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1861 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1921 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1981 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2041 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2101 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2161 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2221 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2281 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2341 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2401 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2461 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2521 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2581 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2641 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2701 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2761 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2821 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2881 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2941 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3001 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3061 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3121 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3181 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3241 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3301 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3361 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3421 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3481 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3541 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3601 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3661 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3721 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3781 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3841 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3901 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3961 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
