LOCUS       sequence-379423-        3861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A9_STOP
FEATURES             Location/Qualifiers
     source          1..3861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1418..1517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1534..1553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1535..1553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1558..1574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1558..1574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1676..1695)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1687..2496
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1754..1773)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2643..3231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3132..3151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3385..3402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3561..3588)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3680..3766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3815..3837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3829..3846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3830..3846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgca gccacctcca
      121 gacgaggcaa ggagagacat ggcaggcgat acccagtgga gcaggcctga gtgtcaggca
      181 tggaccggca cactgctgct gggaacctgc ctgctgtatt gtgccagaag ctccatgcca
      241 atctgcacag tgagcatgtc ccaggacttc ggctggaaca agaaggaggc cggcatcgtg
      301 ctgtctagct tcttttgggg ctactgtctg acccaggtgg tgggaggaca cctgggcgat
      361 aggatcggag gcgagaaagt gatcctgctg tctgccagcg cctggggaag catcaccgca
      421 gtgacaccac tgctggcaca cctgtcctct gcccacctgg ccttcatgac attttccagg
      481 atcctgatgg gactgctgca gggcgtgtat tttcctgccc tgaccagcct gctgtcccag
      541 aaggtgaggg agtctgagcg cgccttcaca tacagcatcg tgggagcagg atcccagttt
      601 ggcaccctgc tgacaggagc agtgggatcc ctgctgctgg agtggtatgg ctggcagtcc
      661 atcttctact tttctggcgg cctgaccctg ctgtgggtgt ggtacgtgta tcggtacctg
      721 ctgagcgaga aggacctgat cctggccctg ggcgtgctgg cacagtctag gccagtgagc
      781 aggcacaaca gagtgccatg gaggcgcctg ttccgcaagc ctgccgtgtg ggcagcagtg
      841 gtgtctcagc tgagcgccgc ctgttctttc tttatcctgc tgagctggct gccaaccttc
      901 tttgaggaga cattccccga cgccaagggc tggatcttta acgtggtgcc atggctggtg
      961 gcaatccctg catccctgtt ctctggcttt ctgtccgatc acctgatcaa tcagggctat
     1021 cgggccatca ccgtgagaaa gctgatgcag ggaatgggac tgggactgag ctccgtgttc
     1081 gcactgtgcc tgggccacac ctctagcttc tgtgagagcg tggtgtttgc ctccgcctct
     1141 atcggcctgc agaccttcaa ccacagcggc atctccgtga atatccagga tctggcacca
     1201 tcctgcgcag gcttcctgtt tggcgtggcc aataccgcag gcgccctggc aggagtggtg
     1261 ggcgtgtgcc tgggcggcta cctgatggag accacaggct cctggacatg tctgttcaac
     1321 ctggtggcca tcatctctaa tctgggcctg tgcaccttcc tggtgtttgg ccaggcccag
     1381 agggtggacc tgtcctctac acacgaggat ctgtaagacc cagctttctt gtacaaagtt
     1441 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1501 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1561 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1621 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1681 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1741 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1801 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1861 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1921 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1981 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2041 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2101 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2161 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2221 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2281 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2341 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2401 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2461 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2521 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2581 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3541 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3601 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3661 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3721 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3781 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3841 gccagtctta agctcgggcc c
//
