LOCUS       sequence-379467-        9943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant ectopic expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-Cam3304-35S
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        181..283
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             314..619
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     498..517
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      707..959
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    981..1005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2306..2935
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3364..4437
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4503..4697
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4933..4955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5041..5181
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5096..5115
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5196..5213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5367..5955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5447..5466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6042..6836)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7261..7285
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7363..7537
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7544..8095)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8140..8816)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    9007..9028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9043..9048,9049..9066,9067..9073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9081..9097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9086..9108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9105..9121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9105..9121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9122..9349
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    9130..9186
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9190..9207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9190..9206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9199..9221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        9583..9928
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agaccgtgtg aattggtgac cagctcgaat ttccccgatc gttcaaacat
      721 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
      781 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
      841 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
      901 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
      961 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     1021 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     1081 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     1141 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     1201 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     1261 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     1321 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     1381 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     1441 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     1501 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     1561 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     1621 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     1681 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcg
     1741 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     1801 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     1861 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     1921 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     1981 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     2041 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     2101 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     2161 cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     2221 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     2281 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     2341 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     2401 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     2461 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     2521 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     2581 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     2641 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     2701 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     2761 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     2821 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     2881 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     2941 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     3001 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     3061 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     3121 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     3181 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     3241 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     3301 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     3361 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     3421 ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     3481 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     3541 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     3601 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     3661 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     3721 gacgggcacg tagaggtttc cgcagggccg gcgggcatgg ccagtgtgtg ggattacgac
     3781 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     3841 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     3901 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     3961 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     4021 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     4081 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     4141 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     4201 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     4261 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     4321 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     4381 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     4441 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     4501 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     4561 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     4621 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     4681 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     4741 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     4801 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     4861 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     4921 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4981 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5041 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     5101 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     5161 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6001 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
     6061 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     6121 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     6181 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
     6241 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
     6301 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
     6361 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     6421 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
     6481 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
     6541 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
     6601 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
     6661 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
     6721 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
     6781 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
     6841 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
     6901 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     6961 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
     7021 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
     7081 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
     7141 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
     7201 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     7261 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     7321 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
     7381 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
     7441 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     7501 cttatctggg aactactcac acattattat ggagaaactc gagtcaaatc tcggtgacgg
     7561 gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat
     7621 gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg
     7681 cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga
     7741 agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc
     7801 gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg
     7861 ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc
     7921 agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct
     7981 cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg
     8041 gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag
     8101 actcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
     8161 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     8221 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     8281 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     8341 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     8401 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     8461 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     8521 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt
     8581 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     8641 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     8701 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     8761 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     8821 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     8881 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
     8941 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9001 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9061 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9121 catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     9181 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9241 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9301 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     9361 cttggatcag attgtcgttt cccgccttca gtttagcttc atggagtcaa agattcaaat
     9421 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     9481 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     9541 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     9601 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     9661 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     9721 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     9781 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     9841 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     9901 aggaagttca tttcatttgg agagaacacg ggggactctt gac
//
