LOCUS       sequence-37948-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Swint-Kruse L, Elam CR, Lin JW, Wycuff DR, Shive Matthews K
  TITLE     Plasticity of quaternary structure: twenty-two ways to form a LacI 
            dimer.
  JOURNAL   Protein Sci. 2001 Feb . 10(2):262-76.
  PUBMED    11266612
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2001 Feb . 10(2):262-76."
COMMENT     Sequence Label: pLS1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(114..130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(127..149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    138..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(162..168,169..186,187..192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(252..269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(503..522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
       61 tgtcggaatt cccggggatc cgtcgacctc gagccaagct tggcgtaatc atggtcatag
      121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      241 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      841 cctaactacg gctacactag aagaacagta tttg
//
