LOCUS       sequence-379490-       11012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LbCas12a expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Lawrenson T, Youles M, Chhetry M, Clarke M, Harwood W, Hundleby P
  TITLE     Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas 
            Systems.
  JOURNAL   Methods Mol Biol. 2023;2653:253-271. doi: 
            10.1007/978-1-0716-3131-7_16.
  PUBMED    36995631
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2023"; volume: "2653:253-271. doi"; pages: " 
            10.1007/978-1-0716-3131-7_16"
COMMENT     Sequence Label: EC59103
FEATURES             Location/Qualifiers
     source          1..11012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    110..134
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      226..857
                     /label=oriV
                     /note="incP origin of replication"
     promoter        944..1048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1049..1117,1118..1909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1267..1286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2080..2668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2569..2588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3004..4152
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4308..4332
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10328..10375
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 acgctaagct tgaattcttg cttgtcttca cagagtgggg cccactgcat ccaccccagt
       61 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
      121 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
      181 cactcgatac aggcagccca tcagtcagat caggatctcc tttgcgacgc tcaccgggct
      241 ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc
      301 gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt
      361 ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc
      421 ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc
      481 gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct
      541 ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc
      601 gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt
      661 tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg
      721 ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa
      781 ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg
      841 aaccctcccg gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg
      901 cgaacggcct caccccaaaa atggcagcgc tggccaattc gtgcgcggaa cccctatttg
      961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cttgataaat
     1021 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1081 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1141 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1201 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1261 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1321 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1381 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1441 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1501 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1561 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1621 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1681 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1741 taaatctgga gccggtgagc gtggttctcg cggtatcatt gcagcactgg ggccagatgg
     1801 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1861 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1921 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1981 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2701 ggcagatcct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
     2761 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
     2821 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
     2881 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
     2941 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
     3001 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     3061 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     3121 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     3181 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt
     3241 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     3301 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     3361 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     3421 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     3481 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     3541 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     3601 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     3661 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     3721 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     3781 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
     3841 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
     3901 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
     3961 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
     4021 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
     4081 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
     4141 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cagcgcctga
     4201 tctggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
     4261 gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa
     4321 tatatcctgt caaacactga tagtttaaac cacttcgtgc agaagacaat agtggaggtc
     4381 gagctgcagg tcaacggatc aggatattct tgtttaagat gttgaactct atggaggttt
     4441 gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag
     4501 aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg
     4561 gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat
     4621 taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca
     4681 ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat
     4741 aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta
     4801 cttttccaag aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa
     4861 aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat
     4921 gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat
     4981 aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc
     5041 ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg
     5101 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa
     5161 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt
     5221 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc
     5281 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc
     5341 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg
     5401 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat
     5461 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaacacga ttttctgggt
     5521 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt
     5581 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct
     5641 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa
     5701 caggaatgag caagctcgag aagtttacca actgctacag cctctctaag accctcaggt
     5761 tcaaggctat ccctgtggga aagacccaag agaatatcga caacaagagg ctcctcgtcg
     5821 aggatgagaa gagagctgaa gattacaagg gcgtgaagaa gctcctcgac aggtactacc
     5881 tcagcttcat caacgatgtg ctccacagca tcaagctcaa gaacctcaac aactacatca
     5941 gcctcttccg taagaaaacc aggaccgaga aagagaacaa agagcttgag aacctcgaga
     6001 tcaacctccg taaagagatc gccaaggctt tcaagggaaa cgagggatac aagagcctct
     6061 tcaagaagga tattatcgag acaatcctgc ctgagttcct ggacgataag gatgagatcg
     6121 ctctcgtgaa cagcttcaac ggattcacta ctgccttcac cggattcttc agaaacaggg
     6181 aaaacatgtt cagcgaagag gccaagagca cctctatcgc tttcagatgc atcaacgaga
     6241 acctcacgcg ttacatcagc aacatggaca tcttcgagaa ggtaacattc cttagttacc
     6301 tttcttttct ttttccatca taagtttata gattgtacat gctttgagat ttttctttgc
     6361 aaacaatctc aggtggacgc catcttcgat aagcacgagg tgcaagaaat caaagagaag
     6421 atcctcaaca gcgactacga cgtcgaggac ttttttgaag gggagttctt caacttcgtt
     6481 ctcacccaag agggcatcga cgtgtacaac gctattatcg gaggattcgt gaccgagtct
     6541 ggggagaaga ttaagggact caacgagtac atcaacctgt acaaccagaa aacgaagcag
     6601 aagctcccga agttcaagcc gctctacaag caggtctgtc tttcctattt catatgttta
     6661 atcctaggaa tttgatcaat tgattgtatg tatgtcgatc ccaagacttt cttgttcact
     6721 tatatcttaa ctctctcttt gctgtttctt gcaggttctc tctgatcgtg agagcctctc
     6781 attttacggt gagggttaca cctctgacga ggaagtgctt gaggttttcc gtaacaccct
     6841 caacaagaac agcgagatct tctcgtccat caagaagttg gagaagcttt tcaagaactt
     6901 cgacgagtac agcagcgctg ggatcttcgt taagaacgga cctgctatca gcaccatcag
     6961 caaggatatt ttcggcgagt ggaacgtgat cagggacaag tggaatgctg agtacgatga
     7021 catccacctc aagaagaagg ctgtcgtcac tgagaagtac gaggatgaca ggcgtaagtc
     7081 gttcaagaag atcggctctt tcagcctcga gcagcttcaa gaatacgctg atgctgatct
     7141 cagcgtggtc gagaagctca aagagatcat catccagaag gtcgacgaga tctacaaggt
     7201 aagttgttac ttatgattgt tttcctctct gctacatgta ttttgttgtt catttctgta
     7261 agatataaga attgagtttt cctctgatga tattattagg tgtacgggtc ctctgagaag
     7321 ttgttcgatg ctgatttcgt cctcgagaag agtctgaaga agaacgacgc tgtcgtcgcg
     7381 atcatgaagg atttgctcga cagcgtgaag tccttcgaga actatatcaa ggccttcttc
     7441 ggagagggca aagagactaa tagggacgag tctttctacg gggatttcgt gctcgcttac
     7501 gatatcctcc tcaaggtgga ccatatctac gacgccatca gaaactacgt gacccagaag
     7561 ccttacagca aggacaagtt caagttgtac tttcagaacc cgcagttcat gggcggatgg
     7621 gacaaagaca aagagacaga ttacagggcc accatcctca ggttagtatc atatgaagaa
     7681 atacctagtt tcagttgatg aatgctattt tctgacctca gttgttctct tttgagaatt
     7741 atttcttttc taatttgcct gatttttcta ttaattcatt aggtacgggt ctaagtacta
     7801 cctggccatc atggacaaga aatacgccaa gtgcctccaa aagatcgaca aggatgacgt
     7861 gaacgggaac tatgagaaga tcaactacaa gctccttccg ggaccgaaca agatgcttcc
     7921 taaggtgttc ttcagcaaga aatggatggc ctactacaac ccgtctgagg acatccagaa
     7981 aatctacaag aacgggacct tcaagaaagg cgacatgttc aacctcaacg actgccacaa
     8041 gctcatcgat ttcttcaagg acagcatctc gcgttacccg aagtggtcta acgcttacga
     8101 ctttaacttc agcgagacag aaaagtacaa ggatatcgcc gggttctacc gtgaggttga
     8161 ggaacagggt tacaaagtta gcttcgagag cgcctccaag aaagaggtaa atcctggtcc
     8221 acacttttac gataaaaaca caagatttta aactatgaac tgatcaataa tcattcctaa
     8281 aagaccacac ttttgttttg tttctaaagt aatttttact gttataacag gttgacaagt
     8341 tggtcgaaga gggcaagctc tacatgttcc agatctataa caaggacttc tccgacaaga
     8401 gccacggaac tcctaacctc catacgatgt acttcaagct gcttttcgac gagaacaacc
     8461 acgggcagat cagactttct ggtggtgctg aactcttcat gcgtagggcc tcactcaaga
     8521 aagaagagtt ggttgttcac ccggccaact ctccaatcgc taacaagaat cctgacaacc
     8581 cgaaaaagac caccacgctg tcttacgacg tctacaagga caaaaggttc agcgaggacc
     8641 agtacgagct tcatatcccg atcgctatca acaagtgccc gaagaacatc ttcaagatca
     8701 ataccgaggt aaggacttct catgaatatt agtggcagat tagtgttgtt aaagtctttg
     8761 gttagataat cgatgcctcc taattgtcca tgttttactg gttttctaca attaaaggtg
     8821 agggtgctgc tcaagcacga tgataaccct tacgtgatcg gaatcgatcg tggtgagaga
     8881 aacctcctct acatcgttgt ggtggacgga aagggaaaca tcgtcgagca gtacagcctg
     8941 aacgagatta tcaacaattt caacggcatc aggatcaaga ccgactacca ctcactcctc
     9001 gataagaaag aaaaagagcg tttcgaggcc aggcagaact ggacttctat cgaaaacatc
     9061 aaagagttga aggccggcta catctctcag gtggtgcata agatctgcga gctggtggaa
     9121 aagtacgatg ctgtgatcgc tcttgaggac ctcaactctg ggttcaagaa cagtagagtg
     9181 aaggtaagtt ctgcatttgg ttatgctcct tgcattttag gtgttcgtcg cacttccatt
     9241 tccatgaata gctaagattt tttttctctg cattcattct tcttgcctca gttctaactg
     9301 tttgtggtat ttttgtttta attattgcta caggttgaga agcaggtcta ccaaaagttc
     9361 gagaagatgc tcatcgacaa gctcaactac atggtggaca aaaagagcaa cccttgcgct
     9421 accggtggtg ctcttaaggg ataccagatc acgaacaagt tcgagtcctt caagagcatg
     9481 agcacccaga acggcttcat cttctatatc cctgcttggc tcaccagcaa gatcgatcct
     9541 tctactggtt tcgtgaacct gctcaagacc aagtacacct cgatcgccga cagcaagaag
     9601 ttcatctcgt ctttcgacag gatcatgtac gtgccggaag aggatctttt cgagttcgct
     9661 ctcgactata agaacttcag caggaccgac gccgactaca ttaagaagtg gaagctctac
     9721 tcctacggga accgtatcag gatcttccga aatccgaaga aaaacaacgt gttcgactgg
     9781 gaagaagtgt gcctcacctc tgcctacaaa gaactgttca acaagtacgg catcaactac
     9841 cagcagggtg atatcagggc tcttttgtgc gagcagagcg acaaggcatt ctacagctca
     9901 ttcatggccc tcatgtctct catgctccag atgaggaact ctatcaccgg aaggaccgat
     9961 gtggacttcc ttatctctcc ggtcaagaac tctgacggga tcttctacga cagccgtaac
    10021 tatgaggctc aagagaacgc tatcctgccg aagaatgctg atgcaaacgg ggcttacaac
    10081 attgcgagaa aggtaaagca actgtgtttt aatcaatttc ttgtcaggat atatggatta
    10141 taacttaatt tttgagaaat ctgtagtatt tggcgtgaaa tgagtttgct ttttggtttc
    10201 tcccgtgtta taggttctct gggctatcgg gcagtttaag aaagcggaag atgagaagct
    10261 ggacaaggtg aagatcgcca tctccaacaa agagtggctt gagtacgctc agacctccgt
    10321 taagcacaag aggcctgctg ctactaagaa agctggtcag gctaagaaga agaaatgagc
    10381 ttgctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
    10441 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
    10501 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
    10561 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
    10621 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
    10681 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
    10741 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
    10801 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
    10861 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
    10921 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
    10981 aatgcatttt atgacttgcc aattgattga ca
//
