LOCUS       sequence-379498-       10372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human TSC1 cDNA (driven by EFS 
            promoter) and a blasticidin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Lee MC, Cai H, Murray CW, Li C, Shue YT, Andrejka L, He AL, Holzem 
            AME, Drainas AP, Ko JH, Coles GL, Kong C, Zhu S, Zhu C, Wang J, van 
            de Rijn M, Petrov DA, Winslow MM, Sage J
  TITLE     A multiplexed in vivo approach to identify driver genes in small 
            cell lung cancer.
  JOURNAL   Cell Rep. 2023 Jan 13;42(1):111990. doi: 
            10.1016/j.celrep.2023.111990.
  PUBMED    36640300
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-13"; volume: "42(1):111990. doi"; pages: " 
            10.1016/j.celrep.2023.111990"
COMMENT     Sequence Label: Lenti-EFS-hTSC1-T2A-Bsd
FEATURES             Location/Qualifiers
     source          1..10372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1978..2189
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    2190..2214
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2215..2224
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5722..5775
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5776..6174
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(6175..6199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6217..6805
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6270..6290)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6688..6699)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6893..7126
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7198..7319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7235..7254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7289..7308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7359..7494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7514..7533)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7515..7533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7543..7560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7543..7559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7552..7574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7701..8156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7788..7807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7998..8019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8182..8286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8287..8355,8356..9147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8505..8524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9318..9906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9807..9826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10060..10077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10194..10215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10230..10235,10236..10253,10254..10260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10268..10284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10273..10295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10292..10308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10292..10308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10327..10347
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10329..10347
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg caagtttgta caaaaaagca ggctgccacc
     2221 atggcccaac aagcaaatgt cggggagctt cttgccatgc tggactcccc catgctgggt
     2281 gtgcgggacg acgtgacagc tgtctttaaa gagaacctca attctgaccg tggccctatg
     2341 cttgtaaaca ccttggtgga ttattacctg gaaaccagct ctcagccggc attgcacatc
     2401 ctgaccacct tgcaagagcc acatgacaag cacctcttgg acaggattaa cgaatatgtg
     2461 ggcaaagccg ccactcgttt atccatcctc tcgttactgg gtcatgtcat aagactgcag
     2521 ccatcttgga agcataagct ctctcaagca cctcttttgc cttctttact aaaatgtctc
     2581 aagatggaca ctgacgtcgt tgtcctcaca acaggcgtct tggtgttgat aaccatgcta
     2641 ccaatgattc cacagtctgg gaaacagcat cttcttgatt tctttgacat ttttggccgt
     2701 ctgtcatcat ggtgcctgaa gaaaccaggc cacgtggcgg aagtctatct cgtccatctc
     2761 catgccagtg tgtacgcact ctttcatcgc ctttatggaa tgtacccttg caacttcgtc
     2821 tcctttttgc gttctcatta cagtatgaaa gaaaacctgg agacttttga agaagtggtc
     2881 aagccaatga tggagcatgt gcgaattcat ccggaattag tgactggatc caaggaccat
     2941 gaactggacc ctcgaaggtg gaagagatta gaaactcatg atgttgtgat cgagtgtgcc
     3001 aaaatctctc tggatcccac agaagcctca tatgaagatg gctattctgt gtctcaccaa
     3061 atctcagccc gctttcctca tcgttcagcc gatgtcacca ccagccctta tgctgacaca
     3121 cagaatagct atgggtgtgc tacttctacc ccttactcca cgtctcggct gatgttgtta
     3181 aatatgccag ggcagctacc tcagactctg agttccccat cgacacggct gataactgaa
     3241 ccaccacaag ctactctttg gagcccatct atggtttgtg gtatgaccac tcctccaact
     3301 tctcctggaa atgtcccacc tgatctgtca cacccttaca gtaaagtctt tggtacaact
     3361 gcaggtggaa aaggaactcc tctgggaacc ccagcaacct ctcctcctcc agccccactc
     3421 tgtcattcgg atgactacgt gcacatttca ctcccccagg ccacagtcac accccccagg
     3481 aaggaagaga gaatggattc tgcaagacca tgtctacaca gacaacacca tcttctgaat
     3541 gacagaggat cagaagagcc acctggcagc aaaggttctg tcactctaag tgatcttcca
     3601 gggtttttag gtgatctggc ctctgaagaa gatagtattg aaaaagataa agaagaagct
     3661 gcaatatcta gagaactttc tgagatcacc acagcagagg cagagcctgt ggttcctcga
     3721 ggaggctttg actctccctt ttaccgagac agtctcccag gttctcagcg gaagacccac
     3781 tcggcagcct ccagttctca gggcgccagc gtgaaccctg agcctttaca ctcctccctg
     3841 gacaagcttg ggcctgacac accaaagcaa gcctttactc ccatagacct gccctgcggc
     3901 agtgctgatg aaagccctgc gggagacagg gaatgccaga cttctttgga gaccagtatc
     3961 ttcactccca gtccttgtaa aattccacct ccgacgagag tgggctttgg aagcgggcag
     4021 cctcccccgt atgatcatct ttttgaggtg gcattgccaa agacagccca tcattttgtc
     4081 atcaggaaga ctgaggagct gttaaagaaa gcaaaaggaa acacagagga agatggtgtg
     4141 ccctctacct ccccaatgga agtgctggac agactgatac agcagggagc agacgcgcac
     4201 agcaaggagc tgaacaagtt gcctttaccc agcaagtctg tcgactggac ccactttgga
     4261 ggctctcctc cttcagatga gatccgcacc ctccgagacc agttgctttt actgcacaac
     4321 cagttactct atgagcgttt taagaggcag cagcatgccc tccggaacag gcggctcctc
     4381 cgcaaggtga tcaaagcagc agctctggag gaacataatg ctgccatgaa agatcagttg
     4441 aagttacaag agaaggacat ccagatgtgg aaggttagtc tgcagaaaga acaagctaga
     4501 tacaatcagc tccaggagca gcgtgacact atggtaacca agctccacag ccagatcaga
     4561 cagctgcagc atgaccgaga ggaattctac aaccagagcc aggaattaca gacgaagctg
     4621 gaggactgca ggaacatgat tgcggagctg cggatagaac tgaagaaggc caacaacaag
     4681 gtgtgtcaca ctgagctgct gctcagtcag gtttcccaaa agctctcaaa cagtgagtcg
     4741 gtccagcagc agatggagtt cttgaacagg cagctgttgg ttcttgggga ggtcaacgag
     4801 ctctatttgg aacaactgca gaacaagcac tcagatacca caaaggaagt agaaatgatg
     4861 aaagccgcct atcggaaaga gctagaaaaa aacagaagcc atgttctcca gcagactcag
     4921 aggcttgata cctcccaaaa acggattttg gaactggaat ctcacctggc caagaaagac
     4981 caccttcttt tggaacagaa gaaatatcta gaggatgtca aactccaggc aagaggacag
     5041 ctgcaggccg cagagagcag gtatgaggct cagaaaagga taacccaggt gtttgaattg
     5101 gagatcttag atttatatgg caggttggag aaagatggcc tcctgaaaaa acttgaagaa
     5161 gaaaaagcag aagcagctga agcagcagaa gaaaggcttg actgttgtaa tgacgggtgc
     5221 tcagattcca tggtagggca caatgaagag gcatctggcc acaacggtga gaccaagacc
     5281 cccaggccca gcagcgcccg gggcagtagt ggaagcagag gtggtggagg cagcagcagc
     5341 agcagcagcg agctttctac cccagagaaa cccccacacc agagggcagg cccattcagc
     5401 agtcggtggg agacgactat gggagaagcg tctgccagca tccccaccac tgtgggctca
     5461 cttcccagtt caaaaagctt cctgggtatg aaggctcgag agttatttcg taataagagc
     5521 gagagccagt gtgatgagga cggcatgacc agtagccttt ctgagagcct aaagacagaa
     5581 ctgggcaaag acttgggtgt ggaagccaag attcccctga acctagatgg ccctcacccg
     5641 tctcccccga ccccggacag tgttggacag ctacatatca tggactacaa tgagactcat
     5701 catgaacaca gcggaagcgg agagggcagg ggaagtcttc taacatgcgg ggacgtggag
     5761 gaaaatcccg gccccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     5821 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     5881 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     5941 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     6001 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     6061 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     6121 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaaacccag
     6181 ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga ttacaaaatt
     6241 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6301 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6361 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6421 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6481 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6541 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6601 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6661 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6721 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6781 atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgcttt aagaccaatg
     6841 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6901 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     6961 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7021 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7081 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7141 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7321 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7381 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7441 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     7501 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     7561 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     7621 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     7681 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7921 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7981 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8041 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8101 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     8161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     9481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9721 gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10081 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10141 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10201 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10261 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    10321 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc tt
//
