LOCUS       sequence-37954-e         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Pereira MJ, Behera V, Walter NG
  TITLE     Nondenaturing purification of co-transcriptionally folded RNA avoids
            common folding heterogeneity.
  JOURNAL   PLoS One. 2010  . 5(9):e12953.
  PUBMED    20886091
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(9):e12953."
COMMENT     Sequence Label: pMCGL-T7Mutant
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(284..300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(284..300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(297..319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    308..324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(332..338,339..356,357..362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(515..532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcatgtgat ttctctttgt tcaaggagtc acccccttgg tttgaagaaa tccttacggc
       61 tgtgatctgc acactcagcc gggaggcatc cgccgaaaat tcgataacct ccatcctcgt
      121 caacttaaac cgcggggcgc gactatctta cgcacttgca tgattctcct cgctgcttaa
      181 gttcgggcgc gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcaagct
      241 tgtctcccta tagtttttag tattagagct tggcgtaatc atggtcatag ctgtttcctg
      301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg tta
//
