LOCUS       sequence-379568-        6753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HA-Bax..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Scorrano L
  TITLE     Luca Scorrano plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pcDNA3.1-HA-Bax
FEATURES             Location/Qualifiers
     source          1..6753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    148..282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(185..204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     239..258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(313..329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(313..329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(326..348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    337..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(361..367,368..385,386..391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    406..427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(544..561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(715..1303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(795..814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1474..2265,2266..2334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2097..2116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2335..2439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2514..2533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2705..3084
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3085..3288
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3239..3259
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3333..3352
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3333..3351
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3377..3386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3389..3415
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4010..4019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4022..4048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4656..4672)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4656..4672)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(4682..4700)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4683..4700)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4720..4737)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4726..4950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4996..5509
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5083..5102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5293..5314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5532..5552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5537..5866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5717..5852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5933..6727
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5987..6006)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6597..6616
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
       61 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
      121 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
      181 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      241 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgtcgacc tcgagagctt
      301 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      421 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1141 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1381 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1441 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1501 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1561 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1621 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1681 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1741 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1801 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1861 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1921 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1981 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2041 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2101 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2161 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2221 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2281 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2341 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2461 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     2521 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     2581 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     2641 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     2701 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     2761 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2821 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2881 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2941 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3001 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3061 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3121 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3181 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3241 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
     3301 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
     3361 gcttgatatc gaattcgcca ccatggcata cccatacgac gtcccagact acgctatgga
     3421 cgggtccggg gagcagcttg ggagcggcgg gcccaccagc tctgaacaga tcatgaagac
     3481 aggggccttt ttgctacagg gtttcatcca ggatcgagca gggaggatgg ctggggagac
     3541 acctgagctg accttggagc agccgcccca ggatgcgtcc accaagaagc tgagcgagtg
     3601 tctccggcga attggagatg aactggacag caatatggag ctgcagagga tgattgctga
     3661 cgtggacacg gactcccccc gagaggtctt cttccgggtg gcagctgaca tgtttgctga
     3721 tggcaacttc aactggggcc gcgtggttgc cctcttctac tttgctagca aactggtgct
     3781 caaggccctg tgcactaaag tgcccgagct gatcagaacc atcatgggct ggacactgga
     3841 cttcctccgt gagcggctgc ttgtctggat ccaagaccag ggtggctggg aaggcctcct
     3901 ctcctacttc gggaccccca catggcagac agtgaccatc tttgtggctg gagtcctcac
     3961 cgcctcgctc accatctgga agaagatggg ctgagaataa gccgaattcg ccaccatggc
     4021 atacccatac gacgtcccag actacgctat ggacgggtcc ggggagcagc ttgggagcgg
     4081 cgggcccacc agctctgaac agatcatgaa gacaggggcc tttttgctac agggtttcat
     4141 ccaggatcga gcagggagga tggctgggga gacacctgag ctgaccttgg agcagccgcc
     4201 ccaggatgcg tccaccaaga agctgagcga gtgtctccgg cgaattggag atgaactgga
     4261 cagcaatatg gagctgcaga ggatgattgc tgacgtggac acggactccc cccgagaggt
     4321 cttcttccgg gtggcagctg acatgtttgc tgatggcaac ttcaactggg gccgcgtggt
     4381 tgccctcttc tactttgcta gcaaactggt gctcaaggcc ctgtgcacta aagtgcccga
     4441 gctgatcaga accatcatgg gctggacact ggacttcctc cgtgagcggc tgcttgtctg
     4501 gatccaagac cagggtggct gggaaggcct cctctcctac ttcgggaccc ccacatggca
     4561 gacagtgacc atctttgtgg ctggagtcct caccgcctcg ctcaccatct ggaagaagat
     4621 gggctgagaa taagccgaat tcctgcagcc cgggggatcc actagttcta gagggcccta
     4681 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
     4741 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4801 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4861 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4921 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4981 cgagggggga tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5041 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5101 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5161 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5221 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5281 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5341 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5401 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taaatatttg
     5461 cttatacaat cttcctgttt ttggggcttt tctgattatc aaccggggtg ggtaccgagc
     5521 tcgaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     5881 ttggatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5941 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6001 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6061 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6121 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6181 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6241 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6301 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6361 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6421 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6481 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6541 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6601 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6661 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6721 cttctgagcg ggactctggg gttcgaaatg acc
//
