LOCUS       sequence-379573-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Fis1 lacking the C-terminal 27 residues (Fis1-DTM).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Scorrano L
  TITLE     Luca Scorrano plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pCI-His-Fis1-DTM
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1831..2210
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2211..2414
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2365..2385
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2411..2435
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2550..2682
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2665..2684
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2727..2745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2772..2789
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3198..3319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3209..3228)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3263..3282
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3500..3955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3587..3606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3797..3818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4022..4041)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4141..4163
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4201..4219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4287..4391
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 gctcgacaga tcttcaatat tggccattag ccatattatt cattggttat atagcataaa
     1741 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     1801 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
     1861 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1921 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1981 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2041 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     2101 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     2161 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2221 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2281 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2341 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2401 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
     2461 atcacagtta aattgctaac gcagtcagtg cttctgacac aacagtctcg aacttaagct
     2521 gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
     2581 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
     2641 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca
     2701 attacagctc ttaaggctag agtacttaat acgactcact ataggctagc ctcgagaatt
     2761 ccaccatggc ccaccaccac caccaccacg aggccgtgct gaacgagctg gtgtctgtgg
     2821 aggacctgct gaagtttgaa aagaaatttc agtctgagaa ggcagcaggc tcggtgtcca
     2881 agagcacgca gtttgagtac gcctggtgcc tggtgcggag caagtacaat gatgacatcc
     2941 gtaaaggcat cgtgctgctc gaggagctgc tgcccaaagg gagcaaggag gaacagcggg
     3001 attacgtctt ctacctggcc gtggggaact accggctcaa ggaatacgag aaggccttaa
     3061 agtacgtccg cgggttgctg cagacagagc cccagaacaa ccaggccaag gaactggagc
     3121 ggctcattga caaggccatg aagaaagatg gactctgatc tagagtcgac ccgggcggcc
     3181 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3241 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3301 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3361 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     3421 ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3481 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     3961 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     4021 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4081 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4141 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4381 aaaggaagag t
//
