LOCUS       sequence-379576-        4496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Fis1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Scorrano L
  TITLE     Luca Scorrano plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pCI-HA-Fis1
FEATURES             Location/Qualifiers
     source          1..4496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..30)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        98..202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(203..271,272..1063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(421..440)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1234..1822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1723..1742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2033..2412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2413..2616
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2567..2587
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2613..2637
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2752..2884
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2867..2886
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2929..2947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3505..3626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3516..3535)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3570..3589
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3807..4262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3894..3913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4104..4125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4329..4348)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4448..4470
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
       61 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      121 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      181 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      241 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      301 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      361 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      421 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      481 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      541 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      601 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      661 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      721 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      781 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      841 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      901 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      961 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1021 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1381 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1861 ttgctggcct tttgctcaca tggctcgaca gatcttcaat attggccatt agccatatta
     1921 ttcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     1981 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttggcattga
     2041 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2101 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2161 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2221 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2281 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2341 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     2401 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     2461 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2521 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     2581 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcact
     2641 agaagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag tgcttctgac
     2701 acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc
     2761 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
     2821 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
     2881 acaggtgtcc actcccagtt caattacagc tcttaaggct agagtactta atacgactca
     2941 ctataggcta gcctcgagaa ttccatgcag gacctgccag gcaacgacaa cagcaccgcc
     3001 ggccttatgg aggccgtgct gaacgagctg gtgtctgtgg aggacctgct gaagtttgaa
     3061 aagaaatttc agtctgagaa ggcagcaggc tcggtgtcca agagcacgca gtttgagtac
     3121 gcctggtgcc tggtgcggag caagtacaat gatgacatcc gtaaaggcat cgtgctgctc
     3181 gaggagctgc tgcccaaagg gagcaaggag gaacagcggg attacgtctt ctacctggcc
     3241 gtggggaact accggctcaa ggaatacgag aaggccttaa agtacgtccg cgggttgctg
     3301 cagacagagc cccagaacaa ccaggccaag gaactggagc ggctcattga caaggccatg
     3361 aagaaagatg gactcgtggg catggccatc gtgggaggca tggccctggg tgtggcggga
     3421 ctggccggac tcatcggact tgctgtgtcc aagtccaaat cctgatctag agtcgacccg
     3481 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     3541 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3601 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3661 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     3721 tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     3781 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4201 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4261 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     4321 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4381 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4441 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgc
//
