LOCUS       sequence-379611-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for protein subcellular 
            localization in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-Cam1304-SubC
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        184..529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        725..827
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             858..1163
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1042..1061
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             join(1221..1223,1224..1226,1227..1940)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1221..1223,1224..1226,1227..1937)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1266..1287)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1527..1546)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1874..1895
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      1963..2215
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2237..2261
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3562..4191
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4620..5693
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5759..5953
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6189..6211)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6297..6437
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6352..6371
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6452..6469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6623..7211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6703..6722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7298..8092)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8517..8541
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8619..8793
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8834..9859)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9926..10602)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    10793..10814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10829..10834,10835..10852,10853..10859)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10867..10883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10872..10894
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10891..10907
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10891..10907
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             10908..11135
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    10916..10972
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10976..10993)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10976..10992)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10985..11007)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
       61 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      121 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      181 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      241 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      301 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      421 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
      541 tgacagtggt ctctgtccag tcctggcctc gtcggccatt ctcgactaag ttggcagcat
      601 cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa
      661 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
      721 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      781 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaactt ttgctgacga
      841 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
      901 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc tgggcgacgg atggtgatcc
      961 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     1021 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtatccg
     1081 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     1141 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagggcc agtctggcca
     1201 cttgtggtct gctgagaccg atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1261 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1321 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1381 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1441 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1501 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1561 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1621 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1681 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1741 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1801 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     1861 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1921 tggacgagct gtacaagtga ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     1981 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2041 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2101 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2161 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2221 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2281 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     2341 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     2401 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     2461 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     2521 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     2581 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     2641 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     2701 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     2761 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     2821 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     2881 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     2941 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag
     3001 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     3061 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     3121 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     3181 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     3241 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     3301 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     3361 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     3421 ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     3481 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     3541 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     3601 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     3661 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     3721 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     3781 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     3841 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     3901 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     3961 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     4021 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     4081 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     4141 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     4201 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     4261 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     4321 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     4381 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     4441 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     4501 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     4561 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     4621 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     4681 tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     4741 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     4801 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     4861 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     4921 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     4981 ggcacgtaga ggtttccgca gggccggcgg gcatggccag tgtgtgggat tacgacctgg
     5041 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     5101 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     5161 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     5221 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     5281 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     5341 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     5401 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     5461 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     5521 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     5581 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     5641 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     5701 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     5761 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     5821 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     5881 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     5941 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     6001 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     6061 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     6121 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     6181 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6241 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     6301 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     6361 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6421 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6481 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6541 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6601 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6661 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6721 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6781 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6841 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6961 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7021 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7081 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7141 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7201 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7261 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
     7321 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     7381 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     7441 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     7501 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     7561 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
     7621 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     7681 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     7741 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     7801 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     7861 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     7921 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     7981 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
     8041 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
     8101 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
     8161 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
     8221 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
     8281 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
     8341 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
     8401 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
     8461 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     8521 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
     8581 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
     8641 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     8701 taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta
     8761 tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatcccg
     8821 gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     8881 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     8941 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     9001 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     9061 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     9121 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     9181 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     9241 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     9301 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     9361 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     9421 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     9481 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
     9541 cggtttcagg caggtcttgc aacgtgacac cctgtgaacg gcgggagatg caataggtca
     9601 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     9661 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     9721 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     9781 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     9841 tgagttcagg ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata
     9901 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
     9961 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
    10021 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    10081 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
    10141 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
    10201 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
    10261 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
    10321 agtagacgag agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt tgaagacgtg
    10381 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    10441 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    10501 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    10561 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    10621 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca
    10681 agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10741 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10801 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10861 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
    10921 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcactgg
    10981 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    11041 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    11101 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
    11161 tcgtttcccg ccttcagttt agctt
//
