LOCUS       sequence-379619-       12140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 transgenic construct that will express human Neuregulin1 Type 
            IIIa in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Lysko DE, Meireles AM, Folland C, McNamara E, Laing NG, Lamont PJ, 
            Ravenscroft G, Talbot WS
  TITLE     Partial loss-of-function variant in neuregulin 1 identified in 
            family with heritable peripheral neuropathy.
  JOURNAL   Hum Mutat. 2022 Sep;43(9):1216-1223. doi: 10.1002/humu.24393. Epub 
            2022 May 17.
  PUBMED    35485770
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/humu.24393"; journalName: "Hum Mutat"; date: "2022-09"; 
            volume: "43"; issue: "9"; pages: "1216-1223"
COMMENT     Sequence Label: pTol2CG NBT:NRGIIIa:polyA
FEATURES             Location/Qualifiers
     source          1..12140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(524..1315,1316..1384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1147..1166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1385..1489)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1652..1673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(2126..2137,2138..2300)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     2673..2689
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2709..2729
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2731..2749
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6297..6313)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6350..6368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6368..6392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6401..6410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8517..8541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8543..8677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8740..8760)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8768..8784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8800..8934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(9236..9949,9950..9952,9953..9955))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9239..9949,9950..9952,9953..9955))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9281..9302)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9630..9649
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9889..9910
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(11178..11365,11366..11377)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11411..11427)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11464..11482)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11503..11519)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11516..11538)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11527..11543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11551..11557,11558..11575,11576..11581))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11596..11617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11734..11751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11905..12140,1..353))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11985..12004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
       61 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taaggcattt
      421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1501 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     1561 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     1621 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     1681 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     1741 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     1801 ccctaaaggg agcccccgat ttagagcttg acggggaaag gctggctaag aactcatcag
     1861 cctccccggt ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac
     1921 atggtgccat tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt
     1981 ggatacgagt acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct
     2041 gatctgcaaa agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt
     2101 cagaggtttg tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa
     2161 gtgaaagtac aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag
     2221 aatcttactt gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga
     2281 agtaaaagta aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa
     2341 aacattaaat gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta
     2401 tcaaactata agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca
     2461 gatttaactt cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg
     2521 ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag
     2581 gtgtattagt cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg
     2641 tgtctgaaac acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc
     2701 caagctatca actttgtata gaaaagttgg taatacgact cactataggg cgaattgggt
     2761 accgggcccc ccctcgaggt cgactctaga ccctgtctgt tcctgattac atctagtttg
     2821 ctgcctgtcc ggattattgc ctgttttttg actactctct tgtatcctgt ggtgtacttt
     2881 ctattttggt gtgtttgact cggcctgtga ccccaaccat ttatttgtct cctgtaactg
     2941 taccgcattg actgattgtt actgacccgg cctggctttt aactacaatt cttgtttcca
     3001 attctacaac cccacacttg gttcccttgc ttggtcagaa ctctctcctt gggcctctca
     3061 cgttaagacc tggcgtcatc tgagtagcag agggctagtc ccgatgccaa aggcggctgt
     3121 tataggcaga agggtgagcc gcgactgggt ccttggtgtc tgttctgatt ttgggacacc
     3181 ctgccttaca gcccccctca agtctctgat tattgccttg taaccttgta accaatcagt
     3241 ggaaatcaag agacctgcaa agcaggaagt agtgttctac ttatgtcact ccagtcttta
     3301 tacattacat ttttggctaa ctaactatat tagaaacatt ttttattttg catagccaat
     3361 ctacagtatt tacccagttt ttatttttaa actgaataat tattttaagg ataatatctg
     3421 cctgcagaat aaataattcg atatatgcca tggataagca atatggtgtc ttgacaagac
     3481 agaatataat ttgatatttt agaagcatgg cttttatgga cttatacaca tgtaggtatg
     3541 atgtgagaac ctggttaatg gtgtccattt gctgtcatga gtttgcttgg ttactgagca
     3601 cagactattt tatgagcttt gccaggtcct gcttttctca tcctttcaaa acaatgtgtt
     3661 cccttcactc cacacagagt aatgtccatg ttcctggtgt gtgagtctcc tcttccaaat
     3721 gcagcatctt ctcccccccc atgccatgtg acactttcat tagcatctcc tcatgctttg
     3781 tgtgaattct gctctgccct acccctcccc ttatgcagca caaggaagaa gggagaaagc
     3841 tgtgtctgca tatctgcaaa aggctgcctg actgacagta agaatgtctg tctcgaggac
     3901 ctcttcccac acagctgtac cactgaaatc catatttatt ggtagtacat ctgttactaa
     3961 cattttaaca ttaacaacag tgattacatt accttcagaa cattgcagta aggaacagac
     4021 tcctgtatgc tcatagtgta ataccaggag aatatattgt aaatcttggg tgttgcccac
     4081 tttcagtgat atcagctttg ctttctgtat tttttaatca tttaatgctc cttcatagta
     4141 atcttgaaat gcaaacattt tccttaaggt gggcatagcc aaacaatatt caacatcaac
     4201 acactgggcc aaatcaggat gatctgatca acaggctaac aatcagatca tatttgggag
     4261 ccatttgcca atgtggtcct cttccaacat gattatccaa actgtctgat agatatatct
     4321 gcttgatagt caaatattgg tcaggcaggc cattgagaag gcctcatgca tgggccaata
     4381 agctgctgat tgggttgcct acttgtggat attttactag cctggccaga aaaaatgttg
     4441 cttgatgcca atgtcaataa ttggaaaaaa acattaaaat ataagaaggc tgttactttt
     4501 ttcccagaaa aggtggtaac cataactgcc aactaattct ggaagagctg agttggcagc
     4561 ttatacttgg ccatttatca atcaccacct ataaggatgt accgagtctg cgtgcttatt
     4621 aggtatatgt gagtatgaca caattttaaa cactattatg tctgcaagag ctgagtcagc
     4681 agttatactt ggccatgtat tgccacccat aaggatgtac tgattcaatc atttttctcc
     4741 aaacccctaa attcatatga tgtctgccag cttattaggt acagtatatg tgagtatgat
     4801 aaaaatattt gtaatattta atattggttg tgttttctct ctatagaaat aatacacaat
     4861 ccaaacaaaa gtggcataaa tgtgatatat tgtaatgaca tgataaggaa attacactga
     4921 tttattggct attaatattt ctcatttgca atcactatgc tgctctggca gcttttatca
     4981 gcacatcagt gctacagaaa gaatggaacc ctggctgtca tgctgattct aatgctcagc
     5041 agcattttac acattttgct gcattttcta gctatcagct gattactccc aggatccccc
     5101 ttgtgttcca ctgcagatgt atttactgat gcttagcttc taccaacggg cacttacaag
     5161 tatttctatg tgggtgtaac tagaaagaag atggactatg taaagcatat gggcctctag
     5221 tttgggcata accttaatgt acatttggat gggaggttcc agggtaaagg ttaatccaat
     5281 agcactggtt tcccctagct ttgccacttt taataggcaa ggtttcagcc ctgtctactc
     5341 atatgtaaat tgtgattgtg attttatttc acatttttgt tctgtatgtt tggacagatg
     5401 tcagtaaaac catttaactg cccccgtttg ttcaacctcc tcgttcttct gctacatcca
     5461 ctggtcttta cggaagcaat taaataaatt aagggccata tgatttcctg ccctgtaaaa
     5521 ctggtgtgtg tttgcggtct gaggcagatg tgctgcttcc atttgaatac aggggggata
     5581 ttgaaacact gactcgggaa tcccctggag agtgatttga ggcagaaatg gatatggagc
     5641 agtatcatgt gataaaatga aattgtgagg cagctgcata gggtggcacc catccccact
     5701 gcaccttcct tccccctaca gctcccagtg atggacgagt tgtgtggggg atggtacctg
     5761 tcactaatac ctttggagtc atcattaggc taatattatc aataacaagg ttaatgtttc
     5821 tataggctta atcagcttct cattcgtgtt ttaattagaa ttttttttca ttatttcagt
     5881 ataatattcc tttgtaataa gcaataagca cattcccagc atctttttca ccttcttggt
     5941 ggtatctgcc tgatggtgag acaatgcagc atccatttcc attccctccc ttgtcatttg
     6001 attggttccc atccctacaa tgctcagggc cagtggctcc acccaccatt acatcatctg
     6061 agcccaggac cacagcaagt gaataggaga gagcagcacc ctccccaagg cgtcacaagt
     6121 gtataaaagg cagcagaagg agcctctgac agcccagatt cttagccggc tggaaaccat
     6181 cggggtgttg cacaaagggc aagtaaagca acaagaaagg gccacaccat ccactgatta
     6241 caccactgta agactcaacc caatcaagct tgatatcgaa ttcctgcagc ccgggggatc
     6301 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     6361 ggttaattca agtttgtaca aaaaagcagg ctccgaattc gccaccatgg agatttattc
     6421 cccagacatg tctgaggtcg ccgccgagag gtcctccagc ccctccactc agctgagtgc
     6481 agacccatct cttgatgggc ttccggcagc agaagacatg ccagagcccc agactgaaga
     6541 tgggagaacc cctggactcg tgggcctggc cgtgccctgc tgtgcgtgcc tagaagctga
     6601 gcgcctgaga ggttgcctca actcagagaa aatctgcatt gtccccatcc tggcttgcct
     6661 ggtcagcctc tgcctctgca tcgccggcct caagtgggta tttgtggaca agatctttga
     6721 atatgactct cctactcacc ttgaccctgg ggggttaggc caggacccta ttatttctct
     6781 ggacgcaact gctgcctcag ctgtgtgggt gtcgtctgag gcatacactt cacctgtctc
     6841 tagggctcaa tctgaaagtg aggttcaagt tacagtgcaa ggtgacaagg ctgttgtctc
     6901 ctttgaacca tcagcggcac cgacaccgaa gaatcgtatt tttgcctttt ctttcttgcc
     6961 gtccactgcg ccatccttcc cttcacccac ccggaaccct gaggtgagaa cgcccaagtc
     7021 agcaactcag ccacaaacaa cagaaactaa tctccaaact gctcctaaac tttctacatc
     7081 tacatccacc actgggacaa gccatcttgt aaaatgtgcg gagaaggaga aaactttctg
     7141 tgtgaatgga ggggagtgct tcatggtgaa agacctttca aacccctcga gatacttgtg
     7201 caagtgccca aatgagttta ctggtgatcg ctgccaaaac tacgtaatgg ccagcttcta
     7261 caagcatctt gggattgaat ttatggaggc ggaggagctg taccagaaga gagtgctgac
     7321 cataaccggc atctgcatcg ccctccttgt ggtcggcatc atgtgtgtgg tggcctactg
     7381 caaaaccaag aaacagcgga aaaagctgca tgaccgtctt cggcagagcc ttcggtctga
     7441 acgaaacaat atgatgaaca ttgccaatgg gcctcaccat cctaacccac cccccgagaa
     7501 tgtccagctg gtgaatcaat acgtatctaa aaacgtcatc tccagtgagc atattgttga
     7561 gagagaagca gagacatcct tttccaccag tcactatact tccacagccc atcactccac
     7621 tactgtcacc cagactccta gccacagctg gagcaacgga cacactgaaa gcatcctttc
     7681 cgaaagccac tctgtaatcg tgatgtcatc cgtagaaaac agtaggcaca gcagcccaac
     7741 tgggggccca agaggacgtc ttaatggcac aggaggccct cgtgaatgta acagcttcct
     7801 caggcatgcc agagaaaccc ctgattccta ccgagactct cctcatagtg aaaggtatgt
     7861 gtcagccatg accaccccgg ctcgtatgtc acctgtagat ttccacacgc caagctcccc
     7921 caaatcgccc ccttcggaaa tgtctccacc cgtgtccagc atgacggtgt ccatgccttc
     7981 catggcggtc agccccttca tggaagaaga gagacctcta cttctcgtga caccaccaag
     8041 gctgcgggag aagaagtttg accatcaccc tcagcagttc agctccttcc accacaaccc
     8101 cgcgcatgac agtaacagcc tccctgctag ccccttgagg atagtggagg atgaggagta
     8161 tgaaacgacc caagagtacg agccagccca agagcctgtt aagaaactcg ccaatagccg
     8221 gcgggccaaa agaaccaagc ccaatggcca cattgctaac agattggaag tggacagcaa
     8281 cacaagctcc cagagcagta actcagagag tgaaacagaa gatgaaagag taggtgaaga
     8341 tacgcctttc ctgggcatac agaaccccct ggcagccagt cttgaggcaa cacctgcctt
     8401 ccgcctggct gacagcagga ctaacccagc aggccgcttc tcgacacagg aagaaatcca
     8461 ggccaggctg tctagtgtaa ttgctaacca agaccctatt gctgtataag aattcgaccc
     8521 agctttcttg tacaaagtgg gggatccaga catgataaga tacattgatg agtttggaca
     8581 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     8641 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     8701 tatgtttcag gttcaggggg aggtgtggga ggttttttcc aactttatta tacatagttg
     8761 ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat gataagatac
     8821 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     8881 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     8941 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     9001 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatctcttgt
     9061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9181 tctggatcta cgtaatacga ctcactatag ttctagaggc tcgagagggg ccgctttact
     9241 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     9301 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     9361 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     9421 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     9481 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     9541 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     9601 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     9661 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     9721 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     9781 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     9841 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     9901 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtca
     9961 ctgtctgctt tgctgttggt ctgggctcct gggtcactgg cttactaatg gagtctttat
    10021 gtatgaggac tcttatcaat tgttcttcta taaaggtctg cagtgtttct gttcgtcccc
    10081 tacatggaca cccagagcct cctaaataca ggagccctga taactgcaca agtgctcaga
    10141 ttccagcagg gtggaaaatg agataaagtg tgcagatggg gagggggacg tgaatgagag
    10201 atttgaggga tgaaaaggat ggatgaacgc attgaaaata gcccctttca caaaataata
    10261 ccagtaaatt gtcatataat taatgaccag acctttacct gtaaatgatg tgatgttcat
    10321 atacagaaga atgccaacaa tttacagatg attttacaaa ttcttaattt tccttttcta
    10381 aatggatttg ttcacacatt atctcttcaa agcaattgaa ttttctggaa aagattggct
    10441 gtgtgtgtaa aaggtgctac tgaggaattt aatgtgacat ggaatgaagc aaacagcagc
    10501 ctaagacagg atggcaggaa aaatgtcaca tgtttaaaat aagagtgaaa ccaacgacct
    10561 ggacttatag agactgtgtg tccttaggca aagctcttgt acaaacagtg cttgtgactg
    10621 tgaaattcgg ggtttgcctg gattgtgtta aatgtgtgct tggatgtatc ataaaaaaga
    10681 atcggttctc tgtatttgac attttctgta atttatctaa agttacaact ggctatgcct
    10741 gatttttatc actattgaat caatcatttt aaagaatgtt aagtctgctg atttggagag
    10801 ctcatttaca tttattttga atgtctctta tttaacacaa ctgatttaag ctttagatct
    10861 gcgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc
    10921 agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc
    10981 cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc
    11041 caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc
    11101 agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag
    11161 caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa
    11221 ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt
    11281 attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag
    11341 taatcaagta aaattactca agtactttac acctctggtt cttgaccccc taccttcagc
    11401 aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
    11461 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
    11521 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11581 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11641 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11701 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11821 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11881 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11941 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    12001 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    12061 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    12121 cagttcggtg taggtcgttc
//
