LOCUS       sequence-379620-       12140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 transgenic construct that will express human Neuregulin1 Type 
            IIIa R551Q patient variant in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Lysko DE, Meireles AM, Folland C, McNamara E, Laing NG, Lamont PJ, 
            Ravenscroft G, Talbot WS
  TITLE     Partial loss-of-function variant in neuregulin 1 identified in 
            family with heritable peripheral neuropathy.
  JOURNAL   Hum Mutat. 2022 Sep;43(9):1216-1223. doi: 10.1002/humu.24393. Epub 
            2022 May 17.
  PUBMED    35485770
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/humu.24393"; journalName: "Hum Mutat"; date: "2022-09"; 
            volume: "43"; issue: "9"; pages: "1216-1223"
COMMENT     Sequence Label: pTol2CG NBT:NRGIIIa_R551Q:polyA
FEATURES             Location/Qualifiers
     source          1..12140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..73)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(526..537,538..700)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1073..1089
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1109..1129
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1131..1149
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4697..4713)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4750..4768)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    4768..4792
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4801..4810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6917..6941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6943..7077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7140..7160)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7168..7184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7200..7334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7636..8349,8350..8352,8353..8355))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7639..8349,8350..8352,8353..8355))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7681..7702)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8030..8049
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8289..8310
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(9578..9765,9766..9777)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9811..9827)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9864..9882)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9903..9919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9916..9938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9927..9943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9951..9957,9958..9975,9976..9981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9996..10017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10134..10151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10305..10893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10385..10404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11064..11855,11856..11924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11687..11706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11925..12029)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
       61 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      121 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      181 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      241 gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat gcaccaattg
      301 gccacaatga cggctactac atggtgccat tccttcctct ttataggaat ggagactacc
      361 tcctgtccaa caatgctctt ggatacgagt acgcctacct gttggaccca ggtcattgca
      421 caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag acacccatat
      481 ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag gtgtaaaaag
      541 tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt actcaattaa
      601 aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac tccattaaaa
      661 ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag agattcttgt
      721 ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta tcctgcttta
      781 gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca gtacacgcta
      841 ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa aattgttagt
      901 gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca
      961 ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa tagcaggaaa
     1021 cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccctcg agcaggaaac
     1081 agctatgacc atgattacgc caagctatca actttgtata gaaaagttgg taatacgact
     1141 cactataggg cgaattgggt accgggcccc ccctcgaggt cgactctaga ccctgtctgt
     1201 tcctgattac atctagtttg ctgcctgtcc ggattattgc ctgttttttg actactctct
     1261 tgtatcctgt ggtgtacttt ctattttggt gtgtttgact cggcctgtga ccccaaccat
     1321 ttatttgtct cctgtaactg taccgcattg actgattgtt actgacccgg cctggctttt
     1381 aactacaatt cttgtttcca attctacaac cccacacttg gttcccttgc ttggtcagaa
     1441 ctctctcctt gggcctctca cgttaagacc tggcgtcatc tgagtagcag agggctagtc
     1501 ccgatgccaa aggcggctgt tataggcaga agggtgagcc gcgactgggt ccttggtgtc
     1561 tgttctgatt ttgggacacc ctgccttaca gcccccctca agtctctgat tattgccttg
     1621 taaccttgta accaatcagt ggaaatcaag agacctgcaa agcaggaagt agtgttctac
     1681 ttatgtcact ccagtcttta tacattacat ttttggctaa ctaactatat tagaaacatt
     1741 ttttattttg catagccaat ctacagtatt tacccagttt ttatttttaa actgaataat
     1801 tattttaagg ataatatctg cctgcagaat aaataattcg atatatgcca tggataagca
     1861 atatggtgtc ttgacaagac agaatataat ttgatatttt agaagcatgg cttttatgga
     1921 cttatacaca tgtaggtatg atgtgagaac ctggttaatg gtgtccattt gctgtcatga
     1981 gtttgcttgg ttactgagca cagactattt tatgagcttt gccaggtcct gcttttctca
     2041 tcctttcaaa acaatgtgtt cccttcactc cacacagagt aatgtccatg ttcctggtgt
     2101 gtgagtctcc tcttccaaat gcagcatctt ctcccccccc atgccatgtg acactttcat
     2161 tagcatctcc tcatgctttg tgtgaattct gctctgccct acccctcccc ttatgcagca
     2221 caaggaagaa gggagaaagc tgtgtctgca tatctgcaaa aggctgcctg actgacagta
     2281 agaatgtctg tctcgaggac ctcttcccac acagctgtac cactgaaatc catatttatt
     2341 ggtagtacat ctgttactaa cattttaaca ttaacaacag tgattacatt accttcagaa
     2401 cattgcagta aggaacagac tcctgtatgc tcatagtgta ataccaggag aatatattgt
     2461 aaatcttggg tgttgcccac tttcagtgat atcagctttg ctttctgtat tttttaatca
     2521 tttaatgctc cttcatagta atcttgaaat gcaaacattt tccttaaggt gggcatagcc
     2581 aaacaatatt caacatcaac acactgggcc aaatcaggat gatctgatca acaggctaac
     2641 aatcagatca tatttgggag ccatttgcca atgtggtcct cttccaacat gattatccaa
     2701 actgtctgat agatatatct gcttgatagt caaatattgg tcaggcaggc cattgagaag
     2761 gcctcatgca tgggccaata agctgctgat tgggttgcct acttgtggat attttactag
     2821 cctggccaga aaaaatgttg cttgatgcca atgtcaataa ttggaaaaaa acattaaaat
     2881 ataagaaggc tgttactttt ttcccagaaa aggtggtaac cataactgcc aactaattct
     2941 ggaagagctg agttggcagc ttatacttgg ccatttatca atcaccacct ataaggatgt
     3001 accgagtctg cgtgcttatt aggtatatgt gagtatgaca caattttaaa cactattatg
     3061 tctgcaagag ctgagtcagc agttatactt ggccatgtat tgccacccat aaggatgtac
     3121 tgattcaatc atttttctcc aaacccctaa attcatatga tgtctgccag cttattaggt
     3181 acagtatatg tgagtatgat aaaaatattt gtaatattta atattggttg tgttttctct
     3241 ctatagaaat aatacacaat ccaaacaaaa gtggcataaa tgtgatatat tgtaatgaca
     3301 tgataaggaa attacactga tttattggct attaatattt ctcatttgca atcactatgc
     3361 tgctctggca gcttttatca gcacatcagt gctacagaaa gaatggaacc ctggctgtca
     3421 tgctgattct aatgctcagc agcattttac acattttgct gcattttcta gctatcagct
     3481 gattactccc aggatccccc ttgtgttcca ctgcagatgt atttactgat gcttagcttc
     3541 taccaacggg cacttacaag tatttctatg tgggtgtaac tagaaagaag atggactatg
     3601 taaagcatat gggcctctag tttgggcata accttaatgt acatttggat gggaggttcc
     3661 agggtaaagg ttaatccaat agcactggtt tcccctagct ttgccacttt taataggcaa
     3721 ggtttcagcc ctgtctactc atatgtaaat tgtgattgtg attttatttc acatttttgt
     3781 tctgtatgtt tggacagatg tcagtaaaac catttaactg cccccgtttg ttcaacctcc
     3841 tcgttcttct gctacatcca ctggtcttta cggaagcaat taaataaatt aagggccata
     3901 tgatttcctg ccctgtaaaa ctggtgtgtg tttgcggtct gaggcagatg tgctgcttcc
     3961 atttgaatac aggggggata ttgaaacact gactcgggaa tcccctggag agtgatttga
     4021 ggcagaaatg gatatggagc agtatcatgt gataaaatga aattgtgagg cagctgcata
     4081 gggtggcacc catccccact gcaccttcct tccccctaca gctcccagtg atggacgagt
     4141 tgtgtggggg atggtacctg tcactaatac ctttggagtc atcattaggc taatattatc
     4201 aataacaagg ttaatgtttc tataggctta atcagcttct cattcgtgtt ttaattagaa
     4261 ttttttttca ttatttcagt ataatattcc tttgtaataa gcaataagca cattcccagc
     4321 atctttttca ccttcttggt ggtatctgcc tgatggtgag acaatgcagc atccatttcc
     4381 attccctccc ttgtcatttg attggttccc atccctacaa tgctcagggc cagtggctcc
     4441 acccaccatt acatcatctg agcccaggac cacagcaagt gaataggaga gagcagcacc
     4501 ctccccaagg cgtcacaagt gtataaaagg cagcagaagg agcctctgac agcccagatt
     4561 cttagccggc tggaaaccat cggggtgttg cacaaagggc aagtaaagca acaagaaagg
     4621 gccacaccat ccactgatta caccactgta agactcaacc caatcaagct tgatatcgaa
     4681 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccag
     4741 cttttgttcc ctttagtgag ggttaattca agtttgtaca aaaaagcagg ctccgaattc
     4801 gccaccatgg agatttattc cccagacatg tctgaggtcg ccgccgagag gtcctccagc
     4861 ccctccactc agctgagtgc agacccatct cttgatgggc ttccggcagc agaagacatg
     4921 ccagagcccc agactgaaga tgggagaacc cctggactcg tgggcctggc cgtgccctgc
     4981 tgtgcgtgcc tagaagctga gcgcctgaga ggttgcctca actcagagaa aatctgcatt
     5041 gtccccatcc tggcttgcct ggtcagcctc tgcctctgca tcgccggcct caagtgggta
     5101 tttgtggaca agatctttga atatgactct cctactcacc ttgaccctgg ggggttaggc
     5161 caggacccta ttatttctct ggacgcaact gctgcctcag ctgtgtgggt gtcgtctgag
     5221 gcatacactt cacctgtctc tagggctcaa tctgaaagtg aggttcaagt tacagtgcaa
     5281 ggtgacaagg ctgttgtctc ctttgaacca tcagcggcac cgacaccgaa gaatcgtatt
     5341 tttgcctttt ctttcttgcc gtccactgcg ccatccttcc cttcacccac ccggaaccct
     5401 gaggtgagaa cgcccaagtc agcaactcag ccacaaacaa cagaaactaa tctccaaact
     5461 gctcctaaac tttctacatc tacatccacc actgggacaa gccatcttgt aaaatgtgcg
     5521 gagaaggaga aaactttctg tgtgaatgga ggggagtgct tcatggtgaa agacctttca
     5581 aacccctcga gatacttgtg caagtgccca aatgagttta ctggtgatcg ctgccaaaac
     5641 tacgtaatgg ccagcttcta caagcatctt gggattgaat ttatggaggc ggaggagctg
     5701 taccagaaga gagtgctgac cataaccggc atctgcatcg ccctccttgt ggtcggcatc
     5761 atgtgtgtgg tggcctactg caaaaccaag aaacagcgga aaaagctgca tgaccgtctt
     5821 cggcagagcc ttcggtctga acgaaacaat atgatgaaca ttgccaatgg gcctcaccat
     5881 cctaacccac cccccgagaa tgtccagctg gtgaatcaat acgtatctaa aaacgtcatc
     5941 tccagtgagc atattgttga gagagaagca gagacatcct tttccaccag tcactatact
     6001 tccacagccc atcactccac tactgtcacc cagactccta gccacagctg gagcaacgga
     6061 cacactgaaa gcatcctttc cgaaagccac tctgtaatcg tgatgtcatc cgtagaaaac
     6121 agtaggcaca gcagcccaac tgggggccca agaggacgtc ttaatggcac aggaggccct
     6181 cgtgaatgta acagcttcct caggcatgcc agagaaaccc ctgattccta ccgagactct
     6241 cctcatagtg aaaggtatgt gtcagccatg accaccccgg ctcgtatgtc acctgtagat
     6301 ttccacacgc caagctcccc caaatcgccc ccttcggaaa tgtctccacc cgtgtccagc
     6361 atgacggtgt ccatgccttc catggcggtc agccccttca tggaagaaga gagacctcta
     6421 cttctcgtga caccaccaag gctgcgggag aagaagtttg accatcaccc tcagcagttc
     6481 agctccttcc accacaaccc cgcgcatgac agtaacagcc tccctgctag ccccttgagg
     6541 atagtggagg atgaggagta tgaaacgacc caagagtacg agccagccca agagcctgtt
     6601 aagaaactcg ccaatagccg gcaggccaaa agaaccaagc ccaatggcca cattgctaac
     6661 agattggaag tggacagcaa cacaagctcc cagagcagta actcagagag tgaaacagaa
     6721 gatgaaagag taggtgaaga tacgcctttc ctgggcatac agaaccccct ggcagccagt
     6781 cttgaggcaa cacctgcctt ccgcctggct gacagcagga ctaacccagc aggccgcttc
     6841 tcgacacagg aagaaatcca ggccaggctg tctagtgtaa ttgctaacca agaccctatt
     6901 gctgtataag aattcgaccc agctttcttg tacaaagtgg gggatccaga catgataaga
     6961 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     7021 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     7081 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcc
     7141 aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacc atcgatgatg
     7201 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7261 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7321 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     7381 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     7441 ctctagatca gatctcttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7501 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7561 catcaatgta tcttatcatg tctggatcta cgtaatacga ctcactatag ttctagaggc
     7621 tcgagagggg ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     7681 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
     7741 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     7801 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     7861 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     7921 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     7981 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     8041 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     8101 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     8161 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     8221 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     8281 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     8341 gcccttgctc accatggtca ctgtctgctt tgctgttggt ctgggctcct gggtcactgg
     8401 cttactaatg gagtctttat gtatgaggac tcttatcaat tgttcttcta taaaggtctg
     8461 cagtgtttct gttcgtcccc tacatggaca cccagagcct cctaaataca ggagccctga
     8521 taactgcaca agtgctcaga ttccagcagg gtggaaaatg agataaagtg tgcagatggg
     8581 gagggggacg tgaatgagag atttgaggga tgaaaaggat ggatgaacgc attgaaaata
     8641 gcccctttca caaaataata ccagtaaatt gtcatataat taatgaccag acctttacct
     8701 gtaaatgatg tgatgttcat atacagaaga atgccaacaa tttacagatg attttacaaa
     8761 ttcttaattt tccttttcta aatggatttg ttcacacatt atctcttcaa agcaattgaa
     8821 ttttctggaa aagattggct gtgtgtgtaa aaggtgctac tgaggaattt aatgtgacat
     8881 ggaatgaagc aaacagcagc ctaagacagg atggcaggaa aaatgtcaca tgtttaaaat
     8941 aagagtgaaa ccaacgacct ggacttatag agactgtgtg tccttaggca aagctcttgt
     9001 acaaacagtg cttgtgactg tgaaattcgg ggtttgcctg gattgtgtta aatgtgtgct
     9061 tggatgtatc ataaaaaaga atcggttctc tgtatttgac attttctgta atttatctaa
     9121 agttacaact ggctatgcct gatttttatc actattgaat caatcatttt aaagaatgtt
     9181 aagtctgctg atttggagag ctcatttaca tttattttga atgtctctta tttaacacaa
     9241 ctgatttaag ctttagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga
     9301 ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac
     9361 tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag
     9421 atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg
     9481 tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa
     9541 ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta
     9601 ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt
     9661 aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca
     9721 aaaataatac ttaagtacag taatcaagta aaattactca agtactttac acctctggtt
     9781 cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg
     9841 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     9901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9961 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10021 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10081 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10141 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10201 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10921 tctgacgctc agtggaacga aaactcacgt taaggcattt tggtcatgag attatcaaaa
    10981 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11041 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11101 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11161 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11221 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11281 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11341 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11401 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11461 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11521 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11581 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11641 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11701 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11761 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11821 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11881 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12001 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
    12061 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
    12121 accaataggc cgaaatcggc
//
