LOCUS       sequence-379621-        9486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of PTK Borealin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9486)
  TITLE     Maddox lab plasmids
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Borealin-mTAG-RFPt-3XFLAG
FEATURES             Location/Qualifiers
     source          1..9486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          585..1587
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1511..1533
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1595..1614
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2548..2557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2554..3285
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             3292..3357
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(3401..3975,3976..3978,3979..3987)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3568..3585)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3795..3814
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3988..4587
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3988..4007)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4484..4504
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4668..4685)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4674..4898
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4945..4961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4969..4985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4993..4999,5000..5017,5018..5023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5038..5059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   5286..5348
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5367..5386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5368..5386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5396..5413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5396..5412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5405..5427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5554..6009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5641..5660)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5851..5872
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6035..6139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6140..6208,6209..7000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6358..6377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7171..7759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7660..7679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7913..7930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7945..8085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8011..8030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8171..8193
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8187..8378)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     protein_bind    8919..8940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8955..8960,8961..8978,8979..8985)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8993..9009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9017..9033
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9052..9072
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9054..9072
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   9095..9129
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        join(9407..9486,1..304)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
      361 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
      421 ggggggggsg cgcgccrggs ggggsggggs ggggsgrggg gsggggsggg gsgrggcggr
      481 gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      541 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      601 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      661 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      721 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      781 gggagggccc tttgtgcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
      841 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
      901 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
      961 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     1021 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     1081 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     1141 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccggggr gggctcgggg
     1201 grggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     1261 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     1321 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     1381 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     1441 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     1501 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     1561 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1621 catcattttg gcaaagaatt cctcgacgct agcaccatgg ccccgtcgaa gaaggcctcg
     1681 agccggacga ccaagaagag cgcggtgcgg ggcagaaagc tcgcctcttt cctcaaagac
     1741 ttcgaccgtg aagtacaaat tagatcgaag caaatacagt cagatgctca gaacctcgtc
     1801 aaagaagtgg acaatcttta taacattgaa atcctccgac ttcccaaggc gcttagagag
     1861 atgaattggc gtgaatattt tgctctagga ggaagcaaac gggccctgga agaagcagca
     1921 acagctgacc tggatatcac agagataaat aagctgacag ctgaagcaat tcagacgccc
     1981 ttgaaagccg tcagagcaaa gaaagcagta aaaatggctg agatgattaa agaagaagaa
     2041 gaagaagaaa ctgaaaatat taatcctcag actgcaaagg caaagaagtg tccaacgacc
     2101 aagaagagga ctcagtcagt gcgggcaaat ggcaggagca aaaggtcaaa ccaaagtaat
     2161 cttgttactc cagccatggg cagatctgat ttctccatgt tgggaccaac tccaggcctg
     2221 acacccaggt ttgattccag gatcttcaag accccaggcc tccgtacccc tgcagccaga
     2281 gaacggattt atagcatctc tgtgaacggc agtcccctgg ctgatagcaa tgaggtcttt
     2341 ctcactgtgc ccatgggcgg aggagagagc atgcacttat tggccagtga tttacaaaag
     2401 gtggatctgg cacatctgga tccagaggcc ttgggaaaca ttaagaagtt atctaatcga
     2461 cttgctcaga tttgtagtag tttacggacc caaaaaacgc gtcgaattct gcagtcgacg
     2521 gtaccgcggg cccgggatcc accggtcgcc accatggtgt ctaagggcga agagctgatt
     2581 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     2641 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     2701 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     2761 agcagaacct tcatcaacca cacccagggc atccccgatt tctttaagca gtccttccct
     2821 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     2881 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     2941 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag
     3001 atgctgtacc ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg
     3061 ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa acccgctaag
     3121 aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc
     3181 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3241 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagccggt cgactacaaa
     3301 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtaa
     3361 gcggccgctc gataagcttg atatcgaatt ccgccccccc cccctctccc tccccccccc
     3421 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     3481 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     3541 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     3601 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     3661 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     3721 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     3781 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     3841 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     3901 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     3961 aaacacgatg ataatatggc cacaaccatg accgagtaca agcccacggt gcgcctcgcc
     4021 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4081 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4141 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4201 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4261 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4321 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4381 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4441 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4501 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     4561 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     4621 aggagcgcac gaccccatgc atcgatgatc tagagctcgc tgatcagcct cgactgtgcc
     4681 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4741 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4801 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4861 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg
     4921 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5101 gtcgtgccag cggatccttt gttactttat agaagaaatt ttgagttttt gttttttttt
     5161 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
     5221 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
     5281 aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct
     5341 ttctagggtt aaagagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     5401 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5461 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5521 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     5581 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5641 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5701 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5761 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5821 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5881 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5941 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6001 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6061 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6301 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6361 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6661 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7861 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7921 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7981 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8041 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     8101 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8161 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8221 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     8281 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     8341 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     8401 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     8461 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     8521 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     8581 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     8641 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     8701 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     8761 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     8821 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgactg gcacgacagg
     8881 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8941 taggcacccc aggctttaca ctttatgctc ccggctcgta tgttgtgtgg aattgtgagc
     9001 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     9061 cctcactaaa gggaacaaaa gctgggtacc ttaaccctag aaagatagtc tgcgtaaaat
     9121 tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt
     9181 gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg tcaatgaggt
     9241 aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca tgattatctt
     9301 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
     9361 acgtgataac ttattatata tatattttct tgttatagat atcgtcgaca ttgattattg
     9421 actagctagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     9481 gttccg
//
