LOCUS       sequence-379625-       14568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for the analysis of promoter 
            activity in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 14568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-121-Pro
FEATURES             Location/Qualifiers
     source          1..14568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5136..5238
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5269..5574
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5453..5472
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             5655..7466
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      7537..7789
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7798..7815)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7798..7814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7807..7829)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7954..7973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8164..8185
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8432..8456
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10052..11200)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11499..12293)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12485..13252
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(13945..14568,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttagtgg tctctgtcca gtcctggcct
     4981 cgtcggccat tctcgactaa gttggcagca tcacccgacg cactttgcgc cgaataaata
     5041 cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg
     5101 aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
     5161 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     5221 ggagctaagg aagctaaact tttgctgacg agaacaggga ctggtgaaat gcagtttaag
     5281 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     5341 attgacacgc ctgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     5401 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     5461 accaccgata tggccagtgt gccggtatcc gttatcgggg aagaagtggc tgatctcagc
     5521 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     5581 ggctccctta tacacagggc cagtctggcc acttgtggtc tgctgagacc ggatccccgg
     5641 gtggtcagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
     5701 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
     5761 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
     5821 ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac
     5881 cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     5941 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     6001 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
     6061 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga
     6121 aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc
     6181 tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag
     6241 actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac
     6301 tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag
     6361 tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     6421 ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag
     6481 tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     6541 atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg
     6601 cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag
     6661 agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg
     6721 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     6781 gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga
     6841 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     6901 cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     6961 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     7021 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     7081 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     7141 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     7201 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     7261 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     7321 cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca
     7381 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     7441 aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcacca tcgtcggcta
     7501 cagcctcggg aattgctacc gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     7561 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     7621 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     7681 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     7741 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattcact
     7801 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7861 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7921 ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc ccttcctttc
     7981 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8041 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     8101 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8161 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     8221 atttataagg gattttgccg atttcggaac caccatcaaa caggattttc gcctgctggg
     8281 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     8341 gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa aacgtccgca
     8401 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     8461 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     8521 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     8581 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     8641 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc
     8701 atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac
     8761 aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga
     8821 ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac tcccctatcc
     8881 attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc
     8941 cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac aactgtaggt
     9001 tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg
     9061 agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact
     9121 aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc
     9181 agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc
     9241 cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac
     9301 agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta
     9361 cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc
     9421 gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata
     9481 ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg
     9541 atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg
     9601 cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg
     9661 ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat
     9721 gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg
     9781 taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa
     9841 tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt
     9901 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga
     9961 agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg
    10021 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
    10081 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
    10141 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
    10201 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
    10261 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
    10321 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
    10381 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
    10441 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
    10501 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
    10561 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
    10621 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
    10681 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
    10741 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
    10801 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcttgcttg gcctcgctga
    10861 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
    10921 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
    10981 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
    11041 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
    11101 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
    11161 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
    11221 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
    11281 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
    11341 tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat
    11401 acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct
    11461 cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa
    11521 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    11581 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    11641 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    11701 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    11761 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    11821 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    11881 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    11941 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    12001 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    12061 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    12121 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    12181 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    12241 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    12301 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    12361 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    12421 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
    12481 tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
    12541 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
    12601 gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac
    12661 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
    12721 tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt
    12781 tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
    12841 cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta aatcgcgcca
    12901 gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc acgtattcgg
    12961 tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt
    13021 gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt
    13081 aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct
    13141 ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt
    13201 catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta cccaattatg
    13261 atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt
    13321 tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct
    13381 gaaatcctta catacggcga tatcttctat ataaaagata tattatctta tcagtattgt
    13441 caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct tggtagcttt
    13501 ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt tcatacctcg
    13561 gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc cgaacacgag
    13621 cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat
    13681 gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc
    13741 ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct
    13801 tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc
    13861 aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc
    13921 ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc
    13981 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
    14041 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
    14101 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
    14161 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
    14221 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
    14281 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
    14341 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
    14401 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
    14461 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
    14521 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccgg
//
