LOCUS       sequence-379649-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dominant negative CnA H151Q mutant protein 
            (catalytically inactive)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Cereghetti GM, Stangherlin A, Martins de Brito O, Chang CR, 
            Blackstone C, Bernardi P, Scorrano L
  TITLE     Dephosphorylation by calcineurin regulates translocation of Drp1 to 
            mitochondria.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15803-8. doi: 
            10.1073/pnas.0808249105. Epub 2008 Oct 6.
  PUBMED    18838687
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0808249105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-10-14- 14"; volume: "105"; issue: "41"; pages: "15803-8"
COMMENT     Sequence Label: pcDNA3.1/Zeo-CnA-H151Q
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(532..1323,1324..1392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1155..1174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1393..1497)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1528..1997
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2062..2261
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        2452..2755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2756..2959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2910..2930
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2956..2980
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           3049..3088
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             3118..3144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3124..3150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          4364..4936
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5068..5087)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5133..5188
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5187..5206)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5527..5547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5532..5861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5712..5847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6218..7012
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6272..6291)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6882..6901
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8057..8526
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9299..9316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9470..9686,1..372))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
       61 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      181 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
      241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      481 ttgcggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
      601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
      661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
      721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1501 atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata tctgtggtaa
     1561 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
     1621 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     1681 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     1741 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     1801 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
     1861 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     1921 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     1981 tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc
     2041 accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt ccgattgtct
     2101 agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat
     2161 ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg
     2221 tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat cccgatcgtt
     2281 ttggactctt tggtgcaccc cccttagagg agggatatgt ggttctggta ggagacgaga
     2341 acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttgggac cgaagccgcg
     2401 ccgcgcgtct tgtctgccag atcccggatc gatccgaatt cgagctcgcc ccgttacata
     2461 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2521 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2581 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2641 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2701 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2761 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2821 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2881 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2941 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     3001 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggggga tcttggtggc
     3061 gtgaaactcc cgcacctctt cggccagcgc cttgtagaag cgcgtatggc ttctagctat
     3121 ccttatgacg tgcctgacta tgccagcctg ggaggacctt ctaggtcgag cgaacccaag
     3181 gcgattgatc ccaagttgtc gactaccgac agggtggtga aagccgttcc atttccgcca
     3241 agtcaccggc tgacagcaaa ggaagtgttt gataacgatg ggaagcctcg tgtggatatc
     3301 ttaaaagcac atctcatgaa ggaaggcagg ctggaagaaa gtgtcgcgtt gagaataata
     3361 acagagggtg cttcgattct ccgacaggaa aaaaacttgc tggatattga tgccccagtc
     3421 acagtttgcg gggacatcca tggacaattc tttgacttga tgaagctctt tgaagtggga
     3481 ggatctcctg ccaacactcg ctacctcttc ttaggggact atgttgacag agggtacttc
     3541 agtatcgaat gtgtgctgta tttgtgggcc ttgaaaattc tttaccccaa aacactgttt
     3601 ttacttcgtg gaaaccatga atgtaggcac ctaacagagt atttcacgtt taaacaagaa
     3661 tgtaaaataa agtattcaga acgcgtttat gacgcctgta tggatgcctt cgactgcttt
     3721 cccctggctg cgctgatgaa ccaacaattc ctgtgtgtac acggtggttt gtctccagag
     3781 attaacactc tagatgacat cagaaaatta gaccgattca aagaaccacc tgcttatggg
     3841 cctatgtgtg acatcttgtg gtcagacccc ctggaggact ttggaaatga gaagactcag
     3901 gaacatttca ctcacaacac agtcaggggt tgttcgtact tctacagtta cccggctgta
     3961 tgtgacttcc tgcagcacaa taatttgttg tccatactcc gagcccacga agcccaggac
     4021 gcagggtacc gcatgtacag gaaaagccaa acaactggct tcccgtctct aattacgatc
     4081 ttctcggcac caaattactt agatgtgtac aataataaag ctgcagtgtt gaagtacgag
     4141 aacaacgtga tgaacatcag gcagttcaac tgctcccctc atccgtactg gctcccaaat
     4201 ttcatggatg ttttcacctg gtcgctgcca tttgttgggg agaaagtgac tgagatgctg
     4261 gtaaacgtcc tgaacatctg ctcagatgat gaactggggt cagaagaaga tggatttgac
     4321 ggagccacgg ctgcagcccg gtagggatcc tgagaacttc agggtgagtt tggggaccct
     4381 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     4441 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
     4501 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
     4561 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     4621 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     4681 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     4741 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     4801 ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     4861 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     4921 cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg
     4981 caaagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5041 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5101 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5161 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5221 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5281 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5341 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5401 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5461 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     5521 ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5581 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5641 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5701 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5761 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5821 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttcac gctgccgcaa
     5881 gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa
     5941 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
     6001 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
     6061 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
     6121 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
     6181 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6241 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6301 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6361 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6421 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6481 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6541 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6601 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6661 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6721 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6781 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6841 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6901 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6961 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     7021 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     7081 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7141 atcctccagc gcggggatct catgctggag ttcttcgccc accccgggta atctgctgat
     7201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7741 ggagcctatg gaaaaacgcc agcaacgccg agatgcgccg cctcgaccag ccatcgatta
     7801 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
     7861 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     7921 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     7981 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     8041 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     8101 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     8161 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     8221 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     8281 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     8341 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     8401 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     8461 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     8521 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     8581 atggccatag tacttaaagt tacattggct tccttgaaat aaacatggag tattcagaat
     8641 gtgtcataaa tatttctaat tttaagatag tatctccatt ggctttctac tttttctttt
     8701 attttttttt gtcctctgtc ttccatttgt tgttgttgtt gtttgtttgt ttgtttgttg
     8761 gttggttggt taattttttt ttaaagatcc tacactatag ttcaagctag actattagct
     8821 actctgtaac ccagggtgac cttgaagtca tgggtagcct gctgttttag ccttcccaca
     8881 tctaagatta caggtatgag ctatcatttt tggtatattg attgattgat tgattgatgt
     8941 gtgtgtgtgt gattgtgttt gtgtgtgtga ttgtgtatat gtgtgtatgg ttgtgtgtga
     9001 ttgtgtgtat gtatgtttgt gtgtgattgt gtgtgtgtga ttgtgcatgt gtgtgtgtgt
     9061 gattgtgttt atgtgtatga ttgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     9121 gtgtgtgttg tgtatatata tttatggtag tgagaggcaa cgctccggct caggtgtcag
     9181 gttggttttt gagacagagt ctttcactta gcttggaatt aattcggatc tgtatgcggt
     9241 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     9301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9661 ctcatagctc acgctgtagg tatctc
//
