LOCUS       sequence-379650-       13654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) 
            for integration-free naive conversion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13654)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 13654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4
FEATURES             Location/Qualifiers
     source          1..13654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1744..2451
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1889..1907)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2157..2176
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2467..2520
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3388..3405
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3394..3411
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3691..3747
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5232..5820
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5285..5305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5703..5714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5911..5930)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5976..6031
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6030..6049)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6392..6408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6392..6408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6405..6427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6416..6432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6440..6446,6447..6464,6465..6470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6485..6506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6595..6628
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7089..9014
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    9182..9215
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      9402..11191
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(11732..11749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11903..12491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11983..12002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12662..13453,13454..13522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13285..13304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13523..13627)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgcccttc
     1741 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     1801 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     1861 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     1921 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     1981 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     2041 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     2101 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     2161 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     2221 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     2281 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     2341 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     2401 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtataa ggcatgcggc
     2461 tccggcgagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca
     2521 atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc
     2581 ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc
     2641 gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc
     2701 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa
     2761 gcgctgacgc tggcggagaa gcggccgttc atcgacgagg ctaagcggct gcgagtgctg
     2821 cacatgcagg accaccccaa ctacaagtac cggccgcggc ggcgcaagca ggtgaagcgg
     2881 ctgaagcggg tggagggcgg cttcctgcac ggcctggctg agccgcaggc ggccgcgctg
     2941 ggccccgagg gcggccgcgt ggccatggac ggcctgggcc tccagttccc cgagcagggc
     3001 ttccccgccg gcccgccgct gctgcctccg cacatgggcg gccactaccg cgactgccag
     3061 agtctgggcg cgcctccgct cgacggctac ccgttgccca cgcccgacac gtccccgctg
     3121 gacggcgtgg accccgaccc ggctttcttc gccgccccga tgcccgggga ctgcccggcg
     3181 gccggcacct acagctacgc gcaggtctcg gactacgctg gccccccgga gcctcccgcc
     3241 ggtcccatgc acccccgact cggcccagag cccgcgggtc cctcgattcc gggcctcctg
     3301 gcgccaccca gcgcccttca cgtgtactac ggcgcgatgg gctcgcccgg ggcgggcggc
     3361 gggcgcggct tccagatgca gccgcaacac cagcaccagc accagcacca gcaccacccc
     3421 ccgggccccg gacagccgtc gccccctccg gaggcactgc cctgccggga cggcacggac
     3481 cccagtcagc ccgccgagct cctcggggag gtggaccgca cggaatttga acagtatctg
     3541 cacttcgtgt gcaagcctga gatgggcctc ccctaccagg ggcatgactc cggtgtgaat
     3601 ctccccgaca gccacggggc catttcctcg gtggtgtccg acgccagctc cgcggtatat
     3661 tactgcaact atcctgacgt gggcaccggt gccaccaact tcagcctgct gaagcaggcc
     3721 ggcgacgtgg aggagaaccc cggccccatg aacaattcgc ccttcaccat ggctgtcagt
     3781 gacgcgctgc tcccatcttt ctccacgttc gcgtctggcc cggcgggaag ggagaagaca
     3841 ctgcgtcaag caggtgcccc gaataaccgc tggcgggagg agctctccca catgaagcga
     3901 cttcccccag tgcttcccgg ccgcccctat gacctggcgg cggcgaccgt ggccacagac
     3961 ctggagagcg gcggagccgg tgcggcttgc ggcggtagca acctggcgcc cctacctcgg
     4021 agagagaccg aggagttcaa cgatctcctg gacctggact ttattctctc caattcgctg
     4081 acccatcctc cggagtcagt ggccgccacc gtgtcctcgt cagcgtcagc ctcctcttcg
     4141 tcgtcgccgt cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cacctatccg
     4201 atccgggccg ggaacgaccc gggcgtggcg ccgggcggca cgggcggagg cctcctctat
     4261 ggcagggagt ccgctccccc tccgacggct cccttcaacc tggcggacat caacgacgtg
     4321 agcccctcgg gcggcttcgt ggccgagctc ctgcggccag aattggaccc ggtgtacatt
     4381 ccgccgcagc agccgcagcc gccaggtggc gggctgatgg gcaagttcgt gctgaaggcg
     4441 tcgctgagcg cccctggcag cgagtacggc agcccgtcgg tcatcagcgt cagcaaaggc
     4501 agccctgacg gcagccaccc ggtggtggtg gcgccctaca acggcgggcc gccgcgcacg
     4561 tgccccaaga tcaagcagga ggcggtctct tcgtgcaccc acttgggcgc tggaccccct
     4621 ctcagcaatg gccaccggcc ggctgcacac gacttccccc tggggcggca gctccccagc
     4681 aggactaccc cgaccctggg tcttgaggaa gtgctgagca gcagggactg tcaccctgcc
     4741 ctgccgcttc ctcccggctt ccatccccac ccggggccca attacccatc cttcctgccc
     4801 gatcagatgc agccgcaagt cccgccgctc cattaccaag agctcatgcc acccggttcc
     4861 tgcatgccag aggagcccaa gccaaagagg ggaagacgat cgtggccccg gaaaaggacc
     4921 gccacccaca cttgtgatta cgcgggctgc ggcaaaacct acacaaagag ttcccatctc
     4981 aaggcacacc tgcgaaccca cacaggtgag aaaccttacc actgtgactg ggacggctgt
     5041 ggatggaaat tcgcccgctc agatgaactg accaggcact accgtaaaca cacggggcac
     5101 cgcccgttcc agtgccaaaa atgcgaccga gcattttcca ggtcggacca cctcgcctta
     5161 cacatgaaga gacattttta aaagggcgaa ttgcctgcag gaattcaagc ttcggggact
     5221 agtcatatga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5281 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5341 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5401 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5461 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5521 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5581 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     5641 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5701 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5761 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5821 atcggtacaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     5881 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     5941 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     6001 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     6061 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     6121 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     6181 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     6241 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     6301 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     6361 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6421 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6481 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6541 gaaacctgtc gtgccagcgg atctcaattc cgatcatatt caataaccct taatataact
     6601 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
     6661 cttaggatca attctcatgt ttgacagctt atcatcgata agctgatcct cacaggccgc
     6721 acccagcttt tcttccgttg ccccagtagc atctctgtct ggtgaccttg aagaggaaga
     6781 ggaggggtcc cgagaatccc catccctacc gtccagcaaa aagggggacg aggaatttga
     6841 ggcctggctt gaggctcagg acgcaaatct tgaggatgtt cagcgggagt tttccgggct
     6901 gcgagtaatt ggtgatgagg acgaggatgg ttcggaggat ggggaatttt cagacctgga
     6961 tctgtctgac agcgaccatg aaggggatga gggtgggggg gctgttggag ggggcaggag
     7021 tctgcactcc ctgtattcac tgagcgtcgt ctaataaaga tgtctattga tctcttttag
     7081 tgtgaatcat gtctgacgag gggccaggta caggacctgg aaatggccta ggagagaagg
     7141 gagacacatc tggaccagaa ggctccggcg gcagtggacc tcaaagaaga gggggtgata
     7201 accatggacg aggacgggga agaggacgag gacgaggagg cggaagacca ggagccccgg
     7261 gcggctcagg atcagggcca agacatagag atggtgtccg gagaccccaa aaacgtccaa
     7321 gttgcattgg ctgcaaaggg acccacggtg gaacaggagc aggagcagga gcgggagggg
     7381 caggagcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg
     7441 caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga ggggcaggag
     7501 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
     7561 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     7621 gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
     7681 caggaggagg ggcaggagca ggaggggcag gaggggcagg aggggcagga gcaggagggg
     7741 caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggaggg gcaggagcag
     7801 gaggggcagg agcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg
     7861 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg
     7921 caggagcagg aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag
     7981 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggaggggcag
     8041 gagcaggagg ggcaggagca ggaggtggag gccggggtcg aggaggcagt ggaggccggg
     8101 gtcgaggagg tagtggaggc cggggtcgag gaggtagtgg aggccgccgg ggtagaggac
     8161 gtgaaagagc caggggggga agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag
     8221 aaaagaggcc caggagtccc agtagtcagt catcatcatc cgggtctcca ccgcgcaggc
     8281 cccctccagg tagaaggcca tttttccacc ctgtagggga agccgattat tttgaatacc
     8341 accaagaagg tggcccagat ggtgagcctg acgtgccccc gggagcgata gagcagggcc
     8401 ccgcagatga cccaggagaa ggcccaagca ctggaccccg gggtcagggt gatggaggca
     8461 ggcgcaaaaa aggagggtgg tttggaaagc atcgtggtca aggaggttcc aacccgaaat
     8521 ttgagaacat tgcagaaggt ttaagagctc tcctggctag gagtcacgta gaaaggacta
     8581 ccgacgaagg aacttgggtc gccggtgtgt tcgtatatgg aggtagtaag acctcccttt
     8641 acaacctaag gcgaggaact gcccttgcta ttccacaatg tcgtcttaca ccattgagtc
     8701 gtctcccctt tggaatggcc cctggacccg gcccacaacc tggcccgcta agggagtcca
     8761 ttgtctgtta tttcatggtc tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg
     8821 cgattaagga ccttgttatg acaaagcccg ctcctacctg caatatcagg gtgactgtgt
     8881 gcagctttga cgatggagta gatttgcctc cctggtttcc acctatggtg gaaggggctg
     8941 ccgcggaggg tgatgacgga gatgacggag atgaaggagg tgatggagat gagggtgagg
     9001 aagggcagga gtgatgtaac ttgttaggag acgccctcaa tcgtattaaa agccgtgtat
     9061 tcccccgcac taaagaataa atccccagta gacatcatgc gtgctgttgg tgtatttctg
     9121 gccatctgtc ttgtcaccat tttcgtcctc ccaacatggg gcaattgccg gaacccttaa
     9181 tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgaaga ggttcactag
     9241 cggatctcaa ttgggcatac ccatgttgtc acgtcactca gctccgcgct caacaccttc
     9301 tcgcgttgga aaacattagc gacatttacc tggtgagcaa tcagacatgc gacggcttta
     9361 gcctggcctc cttaaattca cctaagaatg ggagcaacca gcaggaaaag gacaagcagc
     9421 gaaaattcac gcccccttgg gaggtggcgg catatgcaaa ggatagcact cccactctac
     9481 tactgggtat catatgctga ctgtatatgc atgaggatag catatgctac ccggatacag
     9541 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     9601 attaggatag cctatgctac ccagatataa attaggatag catatactac ccagatatag
     9661 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatatag
     9721 attaggatag catatgctac ccagatatag attaggatag catatgctat ccagatattt
     9781 gggtagtata tgctacccag atataaatta ggatagcata tactacccta atctctatta
     9841 ggatagcata tgctacccgg atacagatta ggatagcata tactacccag atatagatta
     9901 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atataaatta
     9961 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
    10021 ggatagccta tgctacccag atatagatta ggatagcata tgctatccag atatttgggt
    10081 agtatatgct acccatggca acattagccc accgtgctct cagcgacctc gtgaatatga
    10141 ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg tgtaatttgt cctccagatc
    10201 gcagcaatcg cgcccctatc ttggcccgcc cacctactta tgcaggtatt ccccggggtg
    10261 ccattagtgg ttttgtgggc aagtggtttg accgcagtgg ttagcggggt tacaatcagc
    10321 caagttatta cacccttatt ttacagtcca aaaccgcagg gcggcgtgtg ggggctgacg
    10381 cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac ttgtctttgt ttatgggccc
    10441 cattggcgtg gagccccgtt taattttcgg gggtgttaga gacaaccagt ggagtccgct
    10501 gctgtcggcg tccactctct ttccccttgt tacaaataga gtgtaacaac atggttcacc
    10561 tgtcttggtc cctgcctggg acacatctta ataaccccag tatcatattg cactaggatt
    10621 atgtgttgcc catagccata aattcgtgtg agatggacat ccagtcttta cggcttgtcc
    10681 ccaccccatg gatttctatt gttaaagata ttcagaatgt ttcattccta cactagtatt
    10741 tattgcccaa ggggtttgtg agggttatat tggtgtcata gcacaatgcc accactgaac
    10801 cccccgtcca aattttattc tgggggcgtc acctgaaacc ttgttttcga gcacctcaca
    10861 tacaccttac tgttcacaac tcagcagtta ttctattagc taaacgaagg agaatgaaga
    10921 agcaggcgaa gattcaggag agttcactgc ccgctccttg atcttcagcc actgcccttg
    10981 tgactaaaat ggttcactac cctcgtggaa tcctgacccc atgtaaataa aaccgtgaca
    11041 gctcatgggg tgggagatat cgctgttcct taggaccctt ttactaaccc taattcgata
    11101 gcatatgctt cccgttgggt aacatatgct attgaattag ggttagtctg gatagtatat
    11161 actactaccc gggaagcata tgctacccgt ttagggttaa caagggggcc ttataaacac
    11221 tattgctaat gccctcttga gggtccgctt atcggtagct acacaggccc ctctgattga
    11281 cgttggtgta gcctcccgta gtcttcctgg gcccctggga ggtacatgtc ccccagcatt
    11341 ggtgtaagag cttcagccaa gagttacaca taaaggcaat gttgtgttgc agtccacaga
    11401 ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg caaatgtgca catccattta
    11461 taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc gtgtcacatg
    11521 tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca tgcactgccc
    11581 cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg gggatcgatc
    11641 ctctagagtc gacgctagcg gatccgctgc attaatgaat cggccaacgc gcggggagag
    11701 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    11761 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    11821 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    11881 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    11941 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    12001 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    12061 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    12121 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    12181 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    12241 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    12301 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    12361 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    12421 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    12481 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    12541 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    12601 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    12661 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    12721 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    12781 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    12841 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    12901 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    12961 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    13021 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    13081 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    13141 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    13201 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    13261 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    13321 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    13381 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    13441 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    13501 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    13561 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    13621 ttccgcgcac atttccccga aaagtgccac ctgg
//
