LOCUS       sequence-379659-        8201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane,
            e.g. for single molecule fluorescence microscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Sotolongo Bellon J, Birkholz O, Richter CP, Eull F, Kenneweg H, 
            Wilmes S, Rothbauer U, You C, Walter MR, Kurre R, Piehler J
  TITLE     Four-color single-molecule imaging with engineered tags resolves the
            molecular architecture of signaling complexes in the plasma 
            membrane.
  JOURNAL   Cell Rep Methods. 2022 Feb 4;2(2):100165. doi: 
            10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28.
  PUBMED    35474965
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-02-4"; volume: "2(2):100165. doi"; pages: " 
            10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28"
COMMENT     Sequence Label: pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..24)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        196..575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        576..779
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     730..750
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     824..843
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        824..842
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     879..938
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             942..968
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             978..1523
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             join(1545..1547,1548..1550,1551..2261)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLKYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1590..1611)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1851..1870)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2198..2219
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          3938..4167
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4150..4169
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4240..4814,4815..4817,4818..4826)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4407..4424)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4634..4653
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4849..5652
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4912..4931)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5522..5541
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5782..5903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5819..5838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6005..6021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6005..6021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6018..6040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6029..6045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6053..6059,6060..6077,6078..6083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6098..6119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6236..6253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6407..6995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6487..6506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7166..7957,7958..8026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7789..7808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8027..8131)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
       61 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
      121 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
      181 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      241 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      301 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      361 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      421 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      481 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      541 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      601 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      661 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      721 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      781 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      841 gggagaccca agcttggtac cgagctcgga tcgatatcat ggagacagac acactcctgc
      901 tatgggtact gctgctctgg gttccaggtt ccactggtga ctatccatat gatgttccag
      961 attatgctga attcaccatg gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc
     1021 ctctgggcaa gctggaactg tctgggtgcg aacagggcct gcaccgtatc atcttcctgg
     1081 gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg
     1141 gaccagagcc actgatgcag gccaccgcct ggctcaacgc ctactttcac cagcctgagg
     1201 ccatcgagga gttccctgtg ccagccctgc accacccagt gttccagcag gagagcttta
     1261 cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc atcagctaca
     1321 gccacctggc cgccctggcc ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga
     1381 gcggaaatcc cgtgcccatt ctgatcccct gccaccgggt ggtgcagggc gacctggacg
     1441 tggggggcta cgagggcggg ctcgccgtga aagagtggct gctggcccac gagggccaca
     1501 gactgggcaa gcctgggctg ggtcctgcag gtataggcgc gcccatggtg agcaagggcg
     1561 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1621 acaggttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1681 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1741 cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1801 agtccgccat gcccgaaggc tacgtccagg agcgtaccat cttcttcaag gacgacggca
     1861 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1921 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg aaatacaact
     1981 acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     2041 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2101 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2161 ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2221 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggatccctc gagtctaaaa
     2281 atctaaaatc tcctcaaaaa gtagaggtcg acatcataga tgacaacttt atcctgaggt
     2341 ggaacaggag cgatgagtct gtcgggaatg tgactttttc attcgattat caaaaaactg
     2401 ggatggataa ttggataaaa ttgtctgggt gtcagaatat tactagtacc aaatgcaact
     2461 tttcttcact caagctgaat gtttatgaag aaattaaatt gcgtataaga gcagaaaaag
     2521 aaaacacttc ttcatggtat gaggttgact catttacacc atttcgcaaa gctcagattg
     2581 gtcctccaga agtacattta gaagctgaag ataaggcaat agtgatacac atctctcctg
     2641 gaacaaaaga tagtgttatg tgggctttgg atggtttaag ctttacatat agcttactta
     2701 tctggaaaaa ctcttcaggt gtagaagaaa ggattgaaaa tatttattcc agacataaaa
     2761 tttataaact ctcaccagag actacttatt gtctaaaagt taaagcagca ctacttacgt
     2821 catggaaaat tggtgtctat agtccagtac attgtataaa gaccacagtt gaaaatgaac
     2881 tacctccacc agaaaatata gaagtcagtg tccaaaatca gaactatgtt cttaaatggg
     2941 attatacata tgcaaacatg acctttcaag ttcagtggct ccacgccttt ttaaaaagga
     3001 atcctggaaa ccatttgtat aaatggaaac aaatacctga ctgtgaaaat gtcaaaacta
     3061 cccagtgtgt ctttcctcaa aacgttttcc aaaaaggaat ttaccttctc cgcgtacaag
     3121 catctgatgg aaataacaca tctttttggt ctgaagagat aaagtttgat actgaaatac
     3181 aagctttcct acttcctcca gtctttaaca ttagatccct tagtgattca ttccatatct
     3241 atatcggtgc tccaaaacag tctggaaaca cgcctgtgat ccaggattat ccactgattt
     3301 atgaaattat tttttgggaa aacacttcaa atgctgagag aaaaattatc gagaaaaaaa
     3361 ctgatgttac agttcctaat ttgaaaccac tgactgtata ttgtgtgaaa gccagagcac
     3421 acaccatgga tgaaaagctg aataaaagca gtgtttttag tgacgctgta tgtgagaaaa
     3481 caaaaccagg aaatacctct aaaatttggc ttatagttgg aatttgtatt gcattatttg
     3541 ctctcccgtt tgtcatttat gctgcgaaag tcttcttgag atgcatcaat tatgtcttct
     3601 ttccatcact taaaccttct tccagtatag atgagtattt ctctgaacag ccattgaaga
     3661 atcttctgct ttcaacttct gaggaacaaa tcgaaaaatg tttcataatt gaaaatataa
     3721 gcacaattgc tacagtagaa gaaactaatc aaactgatga agatcataaa aaatacagtt
     3781 cccaaactag ccaagattca ggaaattatt ctaatgaaga tgaaagcgaa agtaaaacaa
     3841 gtgaagaact acagcaggac tttgtatgag cggccgcata gataactgat ccagtgtgct
     3901 ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg
     3961 ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc
     4021 tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt
     4081 ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg
     4141 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc
     4201 atgcatctag ggcggccaat tccgcccctc tccccccccc ccctctccct cccccccccc
     4261 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     4321 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     4381 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     4441 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     4501 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     4561 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     4621 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     4681 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     4741 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4801 aacacgatga taagcttgcc acaacccggg ataattcctg cagccaatat gggatcggcc
     4861 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4921 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4981 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5041 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5101 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5161 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5221 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5281 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5341 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5401 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5461 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5521 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5581 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5641 gagttcttct gaggggatca attctctaga taactgatca taatcagcca taccacattt
     5701 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5761 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5881 tccaaactca tcaatgtatc ttaacgcgtc gagtgcattc tagttgtggt ttgtccaaac
     5941 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6001 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6061 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6601 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6841 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8161 tcgacggatc gggagatctc ccgatcccct atggtgcact c
//
