LOCUS       sequence-379661-        8201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, 
            e.g. for single molecule fluorescence microscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Sotolongo Bellon J, Birkholz O, Richter CP, Eull F, Kenneweg H, 
            Wilmes S, Rothbauer U, You C, Walter MR, Kurre R, Piehler J
  TITLE     Four-color single-molecule imaging with engineered tags resolves the
            molecular architecture of signaling complexes in the plasma 
            membrane.
  JOURNAL   Cell Rep Methods. 2022 Feb 4;2(2):100165. doi: 
            10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28.
  PUBMED    35474965
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-02-4"; volume: "2(2):100165. doi"; pages: " 
            10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28"
COMMENT     Sequence Label: pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        176..555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        556..759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     710..730
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     804..823
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        804..822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     859..918
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             922..948
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             958..1503
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             join(1525..1527,1528..1530,1531..2241)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTFGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDDHFLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1570..1591)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1831..1850)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2178..2199
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          3918..4147
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4130..4149
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4220..4794,4795..4797,4798..4806)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4387..4404)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4614..4633
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4829..5632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4892..4911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5502..5521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5762..5883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5985..6001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5985..6001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5998..6020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6009..6025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6033..6039,6040..6057,6058..6063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6078..6099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6216..6233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6387..6975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6467..6486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7146..7937,7938..8006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7769..7788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8007..8111)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(8186..8201,1..4))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
       61 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      121 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      181 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      241 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      301 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      421 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      481 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      541 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      601 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      661 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      721 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      781 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
      841 cgagctcgga tcgatatcat ggagacagac acactcctgc tatgggtact gctgctctgg
      901 gttccaggtt ccactggtga ctatccatat gatgttccag attatgctga attcaccatg
      961 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
     1021 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
     1081 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
     1141 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
     1201 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
     1261 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc
     1321 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
     1381 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg
     1441 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
     1501 ggtcctgcag gtataggcgc gcccatggtg agcaagggcg aggagctgtt caccggggtg
     1561 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1621 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1681 aagctgcccg tgccctggcc caccctcgtg accaccctga cctttggcgt gcagtgcttc
     1741 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1801 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1861 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1921 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1981 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2041 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2101 cccgtgctgc tgcccgacga tcacttcctg agcacccagt ccaaactgag caaagacccc
     2161 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2221 ggcatggacg agctgtacaa gggatccctc gagtctaaaa atctaaaatc tcctcaaaaa
     2281 gtagaggtcg acatcataga tgacaacttt atcctgaggt ggaacaggag cgatgagtct
     2341 gtcgggaatg tgactttttc attcgattat caaaaaactg ggatggataa ttggataaaa
     2401 ttgtctgggt gtcagaatat tactagtacc aaatgcaact tttcttcact caagctgaat
     2461 gtttatgaag aaattaaatt gcgtataaga gcagaaaaag aaaacacttc ttcatggtat
     2521 gaggttgact catttacacc atttcgcaaa gctcagattg gtcctccaga agtacattta
     2581 gaagctgaag ataaggcaat agtgatacac atctctcctg gaacaaaaga tagtgttatg
     2641 tgggctttgg atggtttaag ctttacatat agcttactta tctggaaaaa ctcttcaggt
     2701 gtagaagaaa ggattgaaaa tatttattcc agacataaaa tttataaact ctcaccagag
     2761 actacttatt gtctaaaagt taaagcagca ctacttacgt catggaaaat tggtgtctat
     2821 agtccagtac attgtataaa gaccacagtt gaaaatgaac tacctccacc agaaaatata
     2881 gaagtcagtg tccaaaatca gaactatgtt cttaaatggg attatacata tgcaaacatg
     2941 acctttcaag ttcagtggct ccacgccttt ttaaaaagga atcctggaaa ccatttgtat
     3001 aaatggaaac aaatacctga ctgtgaaaat gtcaaaacta cccagtgtgt ctttcctcaa
     3061 aacgttttcc aaaaaggaat ttaccttctc cgcgtacaag catctgatgg aaataacaca
     3121 tctttttggt ctgaagagat aaagtttgat actgaaatac aagctttcct acttcctcca
     3181 gtctttaaca ttagatccct tagtgattca ttccatatct atatcggtgc tccaaaacag
     3241 tctggaaaca cgcctgtgat ccaggattat ccactgattt atgaaattat tttttgggaa
     3301 aacacttcaa atgctgagag aaaaattatc gagaaaaaaa ctgatgttac agttcctaat
     3361 ttgaaaccac tgactgtata ttgtgtgaaa gccagagcac acaccatgga tgaaaagctg
     3421 aataaaagca gtgtttttag tgacgctgta tgtgagaaaa caaaaccagg aaatacctct
     3481 aaaatttggc ttatagttgg aatttgtatt gcattatttg ctctcccgtt tgtcatttat
     3541 gctgcgaaag tcttcttgag atgcatcaat tatgtcttct ttccatcact taaaccttct
     3601 tccagtatag atgagtattt ctctgaacag ccattgaaga atcttctgct ttcaacttct
     3661 gaggaacaaa tcgaaaaatg tttcataatt gaaaatataa gcacaattgc tacagtagaa
     3721 gaaactaatc aaactgatga agatcataaa aaatacagtt cccaaactag ccaagattca
     3781 ggaaattatt ctaatgaaga tgaaagcgaa agtaaaacaa gtgaagaact acagcaggac
     3841 tttgtatgag cggccgcata gataactgat ccagtgtgct ggaattaatt cgctgtctgc
     3901 gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag
     3961 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt
     4021 gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg
     4081 tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc
     4141 tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccaat
     4201 tccgcccctc tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc
     4261 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     4321 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     4381 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     4441 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     4501 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     4561 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     4621 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     4681 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     4741 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc
     4801 acaacccggg ataattcctg cagccaatat gggatcggcc attgaacaag atggattgca
     4861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca
     5401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatca
     5641 attctctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttaacgcgtc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5941 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6001 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6061 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     8161 ccgatcccct atggtgcact ctcagtacaa tctgctctga t
//
