LOCUS       sequence-379668-       10909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  episomal vector expressing mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 10909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pCXLE-mCherry
FEATURES             Location/Qualifiers
     source          1..10909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1739..2449
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1884..1902)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2152..2171
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2487..3075
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2540..2560)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2958..2969)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3166..3185)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3231..3286
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3285..3304)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3647..3663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3647..3663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3660..3682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3671..3687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3695..3701,3702..3719,3720..3725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3740..3761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    3850..3883
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4344..6266
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGG
                     GAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGG
                     SGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPP
                     GRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRR
                     KKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLY
                     NLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKD
                     AIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEG
                     EEGQE"
     protein_bind    6434..6467
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      6654..8443
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8984..9001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9155..9743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9235..9254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9914..10705,10706..10774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10537..10556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10775..10879)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcggccgcat
     1741 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1801 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1861 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1921 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1981 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2041 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     2101 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     2161 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     2221 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     2281 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     2341 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     2401 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa tcgaagaatt
     2461 caagcttcgg ggactagtca tatgataatc aacctctgga ttacaaaatt tgtgaaagat
     2521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2881 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3061 gggccgcctc cccgcatcgg tacaattcac tcctcaggtg caggctgcct atcagaaggt
     3121 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     3181 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     3241 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3301 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     3361 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     3421 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     3481 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     3541 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     3601 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     3661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3781 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatctc aattccgatc atattcaata
     3841 acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct gaagaggagt
     3901 ttacgtccag ccaagcttag gatcaattct catgtttgac agcttatcat cgataagctg
     3961 atcctcacag gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga
     4021 ccttgaagag gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg
     4081 ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg
     4141 ggagttttcc gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga
     4201 attttcagac ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt
     4261 tggagggggc aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct
     4321 attgatctct tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg
     4381 gcctaggaga gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa
     4441 gaagaggggg tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa
     4501 gaccaggagc cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac
     4561 cccaaaaacg tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag
     4621 caggagcggg aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag
     4681 gaggggcagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     4741 gaggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag
     4801 gggcaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag
     4861 gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg
     4921 caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg
     4981 caggagcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
     5041 gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg
     5101 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     5161 gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga ggggcaggag
     5221 gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag
     5281 gaggaggggc aggagcagga ggggcaggag caggaggtgg aggccggggt cgaggaggca
     5341 gtggaggccg gggtcgagga ggtagtggag gccggggtcg aggaggtagt ggaggccgcc
     5401 ggggtagagg acgtgaaaga gccagggggg gaagtcgtga aagagccagg gggagaggtc
     5461 gtggacgtgg agaaaagagg cccaggagtc ccagtagtca gtcatcatca tccgggtctc
     5521 caccgcgcag gccccctcca ggtagaaggc catttttcca ccctgtaggg gaagccgatt
     5581 attttgaata ccaccaagaa ggtggcccag atggtgagcc tgacgtgccc ccgggagcga
     5641 tagagcaggg ccccgcagat gacccaggag aaggcccaag cactggaccc cggggtcagg
     5701 gtgatggagg caggcgcaaa aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt
     5761 ccaacccgaa atttgagaac attgcagaag gtttaagagc tctcctggct aggagtcacg
     5821 tagaaaggac taccgacgaa ggaacttggg tcgccggtgt gttcgtatat ggaggtagta
     5881 agacctccct ttacaaccta aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta
     5941 caccattgag tcgtctcccc tttggaatgg cccctggacc cggcccacaa cctggcccgc
     6001 taagggagtc cattgtctgt tatttcatgg tctttttaca aactcatata tttgctgagg
     6061 ttttgaagga tgcgattaag gaccttgtta tgacaaagcc cgctcctacc tgcaatatca
     6121 gggtgactgt gtgcagcttt gacgatggag tagatttgcc tccctggttt ccacctatgg
     6181 tggaaggggc tgccgcggag ggtgatgacg gagatgacgg agatgaagga ggtgatggag
     6241 atgagggtga ggaagggcag gagtgatgta acttgttagg agacgccctc aatcgtatta
     6301 aaagccgtgt attcccccgc actaaagaat aaatccccag tagacatcat gcgtgctgtt
     6361 ggtgtatttc tggccatctg tcttgtcacc attttcgtcc tcccaacatg gggcaattgc
     6421 cggaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtccctcgaa
     6481 gaggttcact agcggatctc aattgggcat acccatgttg tcacgtcact cagctccgcg
     6541 ctcaacacct tctcgcgttg gaaaacatta gcgacattta cctggtgagc aatcagacat
     6601 gcgacggctt tagcctggcc tccttaaatt cacctaagaa tgggagcaac cagcaggaaa
     6661 aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca aaggatagca
     6721 ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat agcatatgct
     6781 acccggatac agattaggat agcatatact acccagatat agattaggat agcatatgct
     6841 acccagatat agattaggat agcctatgct acccagatat aaattaggat agcatatact
     6901 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
     6961 acccagatat agattaggat agcatatgct acccagatat agattaggat agcatatgct
     7021 atccagatat ttgggtagta tatgctaccc agatataaat taggatagca tatactaccc
     7081 taatctctat taggatagca tatgctaccc ggatacagat taggatagca tatactaccc
     7141 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
     7201 agatataaat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
     7261 agatatagat taggatagcc tatgctaccc agatatagat taggatagca tatgctatcc
     7321 agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct ctcagcgacc
     7381 tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg tgtgtaattt
     7441 gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact tatgcaggta
     7501 ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt ggttagcggg
     7561 gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca gggcggcgtg
     7621 tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc acttgtcttt
     7681 gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta gagacaacca
     7741 gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata gagtgtaaca
     7801 acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc agtatcatat
     7861 tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac atccagtctt
     7921 tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat gtttcattcc
     7981 tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca tagcacaatg
     8041 ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa ccttgttttc
     8101 gagcacctca catacacctt actgttcaca actcagcagt tattctatta gctaaacgaa
     8161 ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct tgatcttcag
     8221 ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc ccatgtaaat
     8281 aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc ttttactaac
     8341 cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt agggttagtc
     8401 tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt aacaaggggg
     8461 ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag ctacacaggc
     8521 ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg gaggtacatg
     8581 tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca atgttgtgtt
     8641 gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt ggcaaatgtg
     8701 cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt tggtcccccc
     8761 ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact gaagggatta
     8821 catgcactgc cccgcgaaga aggggcagag atgtcgtagt caggtttagt tcgtccgggg
     8881 cggggatcga tcctctagag tcgacgctag cggatccgct gcattaatga atcggccaac
     8941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtc
//
