LOCUS       sequence-379757-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control plasmid for the MscL tension biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Hsu YY, Resto Irizarry AM, Fu J, Liu AP
  TITLE     Mechanosensitive Channel-Based Optical Membrane Tension Reporter.
  JOURNAL   ACS Sens. 2023 Jan 27;8(1):12-18. doi: 10.1021/acssensors.2c01921. 
            Epub 2023 Jan 6.
  PUBMED    36608338
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.2c01921"; journalName: "ACS Sens"; date: 
            "2023-01-27- 27"; volume: "8"; issue: "1"; pages: "12-18"
COMMENT     Sequence Label: MscL(G22S)-c-term-cpGFP-ERexp
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     633..654
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1021..1040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgagcatta ttaaagaatt tcgcgaattt gcgatgcgcg ggaacgtggt ggatttggcg
       61 gtgagcgtca ttatcggtgc ggcattcggg aagattgtct cttcactggt tgccgatatc
      121 atcatgcctc ctctgggctt attaattggc gggatcgatt ttaaacagtt tgctgtcacg
      181 ctacgcgatg cgcaggggga tatccctgct gttgtgatgc attacggtgt cttcattcaa
      241 aacgtctttg attttctgat tgtggccttt gccatcttta tggcgattaa gctaatcaac
      301 aaactgaatc ggaaaaaaga agaaccagca gccgcacctg caccaactaa agaagaagta
      361 ttactgacag aaattcgtga tttgctgaaa gagcagaata accgctctgg aagcggagga
      421 tccgggaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttcaag
      481 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
      541 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccata
      601 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      661 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
      721 agcaagggcg aggagctgtt caccggggtg gtgcccatcc aggtcgagct ggacggcgac
      781 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
      841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      901 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      961 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     1021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1081 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1141 gagtacaaca agaaaggcag cttttgctat gaaaacgaag tg
//
