LOCUS       sequence-379760-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD1 with an N-terminal HiBiT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD1
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggagctcgtc gtggctaacg tagagaacca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttgcca ccatgcggct
      121 ctgcatcccg caggtgctgt tggccttgtt cctttccatg ctgacagggc cgggagaagg
      181 cagccgggtg agcggctggc ggctgttcaa gaagattagc ggatcccagg ctgctggtca
      241 gggacctggg caagggcccg ggccggggca acaacctcct ccacccccac aacagcagca
      301 aagcggacaa caatataatg gggaacgtgg gatctccgtt cctgatcacg ggtactgcca
      361 acctatctct attccgctct gcactgatat cgcgtacaac caaacaatca tgccaaactt
      421 gctgggccat actaatcagg aagatgcagg cctcgaagtc catcagttct atccgctcgt
      481 caaggttcaa tgttctgccg aactcaagtt ctttctttgc tctatgtatg caccagtgtg
      541 taccgtactg gagcaagctc tgcccccatg ccggtcactc tgtgaacgtg ccagacaagg
      601 ttgcgaggca ttgatgaata aattcgggtt tcagtggcca gataccctga aatgcgagaa
      661 gttccccgtt catggggctg gcgaactttg cgtcgggcaa aataccagtg acaaaggcac
      721 gccaacaccg tccttgctgc cggagttttg gacatcaaac cctcaacatg gtggtggtgg
      781 tcatcgcggc ggtttccctg gcggggccgg cgcgtccgaa cggggcaaat tctcttgtcc
      841 gagggcactt aaggtgccat catatctgaa ttatcacttt ctcggggaaa aggattgcgg
      901 cgctccgtgt gaacctacga aggtgtatgg cctcatgtat tttgggccag aggaacttcg
      961 ttttagtcgt acctggattg ggatttggtc cgtcctttgc tgcgcctcta ccctgtttac
     1021 ggttctgacg tacttggtcg atatgcgccg gttcagttat ccagaaaggc ccatcatatt
     1081 cttgtccggc tgctatactg cggtagctgt ggcctaaatt gctggctttc ttcccgaaga
     1141 tcgcgttg
//
