LOCUS       sequence-379762-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD2 with an N-terminal HiBiT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD2
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaagcaggag ctctctggcg taactagaga accactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgc
      121 ggctctgcat cccgcaggtg ctgttggcct tgttcctttc catgctgaca gggccgggag
      181 aaggcagccg ggtgagcggc tggcggctgt tcaagaagat tagcggatcc tttcatggag
      241 aaaaaggcat cagcattcct gaccacgggt tttgccaacc catcagtata cccttgtgta
      301 ctgacatcgc ctacaatcag acgattatgc caaacttgtt gggccacact aaccaagaag
      361 atgcgggact cgaggttcat cagttctatc cacttgtcaa agttcaatgc agtcccgagc
      421 tgcgcttttt cctgtgctct atgtacgcgc ctgtttgcac ggtgcttgaa caagcaattc
      481 caccctgtcg cagcatttgc gaacgagcta ggcaaggctg cgaagccctt atgaacaaat
      541 tcgggttcca gtggcccgaa cggttgcgat gtgagcactt tccgcgacac ggcgctgaac
      601 agatatgcgt tgggcaaaac cacagtgagg atggtgcacc ggctctgctg acaacagcgc
      661 ccccacctgg cctccaacca ggagctggtg gcacgcctgg tgggcctggg ggagggggtg
      721 cgcctccacg gtatgccacg ctcgagcatc cgtttcattg tccacgggtc cttaaggtac
      781 cgagttatct gagctacaag tttctgggcg agcgcgattg cgccgcaccc tgtgagcctg
      841 cgaggccgga tggctccatg tttttcagcc aggaagagac gagatttgca aggctctgga
      901 ttctgacctg gtccgtgctg tgttgcgcgt ctacattttt tacggttacg acgtaccttg
      961 tggatatgca gaagatttag gtatcctgag agaccgatta tctttcttac cgggtgttat
     1021 accatgggcc tccgttgcat atattgccgg atttgttctc caagaaag
//
