LOCUS       sequence-379764-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD4 with an N-terminal HiBiT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD4
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataggcagag ctctctggct aactagagaa ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg ccaccatgcg
      121 gctctgcatc ccgcaggtgc tgttggcctt gttcctttcc atgctgacag ggccgggaga
      181 aggcagccgg gtgagcggct ggcggctgtt caagaagatt agcggatcct tcggggacga
      241 ggaagagcgg cgctgcgacc ccatccgcat ctccatgtgc cagaacctcg gctacaacgt
      301 gaccaagatg cccaacctgg ttgggcacga gctgcagacg gacgccgagc tgcagctgac
      361 aactttcaca ccgctcatcc agtacggctg ctccagccag ctgcagttct tcctttgttc
      421 tgtttatgtg ccaatgtgca cagagaagat caacatcccc attggcccat gcggcggcat
      481 gtgtctttca gtcaagagac gctgtgaacc cgtcctgaag gaatttggat ttgcctggcc
      541 agagagtctg aactgcagca aattcccacc acagaacgac cacaaccaca tgtgcatgga
      601 agggccaggt gatgaagagg tgcccttacc tcacaaaacc cccatccagc ctggggaaga
      661 gtgtcactct gtgggaacca attctgatca gtacatctgg gtgaaaagga gcctgaactg
      721 tgtgctcaag tgtggctatg atgctggctt atacagccgc tcagccaagg agttcactga
      781 tatctggatg gctgtgtggg ccagcctgtg tttcatctcc actgccttca cagtactgac
      841 cttcctgatc gattcttcta ggttttccta ccctgagcgc cccatcatat ttctcagtat
      901 gtgctataat atttatagca ttgcttatat tgtcaggctg actgtaggcc gggaaaggat
      961 atcctgtgat tttgaagagg cagcagaacc tgttctcatc caagaaggac ttaagaacac
     1021 aggatgtgca ataattttct tgctgatgta cttttttgga atggccagct ccatttggtg
     1081 ggattattct gaccctccct tggtttttgg cagcaggact caaatggggt catgaagcca
     1141 ttgaaatgcc g
//
