LOCUS       sequence-379766-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD5 with an N-terminal HiBiT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD5
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaaagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgc
      121 ggctctgcat cccgcaggtg ctgttggcct tgttcctttc catgctgaca gggccgggag
      181 aaggcagccg ggtgagcggc tggcggctgt tcaagaagat tagcggatcc gcctctaagg
      241 cccctgtgtg ccaagagatc accgtgccta tgtgcagagg catcggctac aacctgacac
      301 acatgcccaa ccagttcaac cacgacaccc aggatgaggc cggactggaa gtgcaccagt
      361 tttggcccct ggtggaaatc cagtgtagcc ccgacctgcg gttcttcctg tgctccatgt
      421 acacccctat ctgcctgcct gactaccaca agcctctgcc tccatgcaga tccgtgtgcg
      481 agagggccaa agccggatgt agccctctga tgagacagta cggcttcgcc tggcctgagc
      541 ggatgagctg tgatagactg cctgtgctgg gcagagatgc cgaggtgctg tgcatggact
      601 acaacagaag cgaggccacc accgctccac ctagaccttt tccagccaag cctacactgc
      661 ctggacctcc aggtgctcct gcttctggcg gagaatgtcc tgctggcgga cctttcgtgt
      721 gcaagtgcag agaacccttc gtgcccatcc tgaaagagag ccatcctctg tacaacaaag
      781 tgcggaccgg ccaggtgcca aattgcgccg ttccttgtta ccagcctagc ttcagcgccg
      841 acgagagaac cttcgccacc ttctggatcg gcctttggag cgtgctgtgc ttcatcagca
      901 cctctacaac cgtggccacc ttcctgatcg acatggaacg gttcagatac cccgagcggc
      961 ctatcatctt cctgagcgcc tgttacctgt gcgtgtccct gggatttctc gtgcggctgg
     1021 ttgtgggaca tgccagcgtt gcctgtagca gagagcacaa ccacatccac tacgagacaa
     1081 caggccccgc tctgtgcacc atcgtgtttc tgctggtgta cttcttcggc atggccagct
     1141 ccatttggtg ggtcatcctg aacctgacct ggtttctggc cgctggaatg aagtggggca
     1201 acgaggccat tgccggctac gcccagaatt ttc
//
