LOCUS       sequence-379768-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD6 with an N-terminal HiBiT; contains M345L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD6
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggcaatag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta agcttgccac
      121 catgcggctc tgcatcccgc aggtgctgtt ggccttgttc ctttccatgc tgacagggcc
      181 gggagaaggc agccgggtga gcggctggcg gctgttcaag aagattagcg gatcccacag
      241 tctcttcacc tgtgaaccaa ttactgttcc cagatgtatg aaaatggcct acaacatgac
      301 gtttttccct aatctgatgg gtcattatga ccagagtatt gccgcggtgg aaatggagca
      361 ttttcttcct ctcgcaaatc tggaatgttc accaaacatt gaaactttcc tctgcaaagc
      421 atttgtacca acctgcatag aacaaattca tgtggttcca ccttgtcgta aactttgtga
      481 gaaagtatat tctgattgca aaaaattaat tgacactttt ggaatccgat ggcctgagga
      541 gcttgaatgt gacagattac aatactgtga tgagactgtt cctgtaactt ttgatccaca
      601 cacagaattt cttggtcctc agaagaaaac agaacaagtc caaagagaca ttggattttg
      661 gtgtccaagg catcttaaga cttctggggg acaaggatat aagtttctgg gaattgacca
      721 gtgtgcgcct ccatgcccca acatgtattt taaaagtgat gagctagagt ttgcaaaaag
      781 ttttattgga acagtttcaa tattttgtct ttgtgcaact ctgttcacat tccttacttt
      841 tttaattgat gttagaagat tcagataccc agagagacca attatatatt actctgtctg
      901 ttacagcatt gtatctctta tgtacttcat tggatttttg ctgggcgata gcacagcctg
      961 caataaggca gatgagaagc tagaacttgg tgacactgtt gtcctaggct ctcaaaataa
     1021 ggctggcacc gttttgttca tgcttttgta ttttttcaaa tggctggcac tggggtggtg
     1081 ggtgattctt accattaact gggtcttagc tgcaggaaga aaatggaatt gtgaagccat
     1141 cgagcaaaa
//
