LOCUS       sequence-379772-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD8 with an N-terminal HiBiT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD8
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taagcaagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgc
      121 ggctctgcat cccgcaggtg ctgttggcct tgttcctttc catgctgaca gggccgggag
      181 aaggcagccg ggtgagcggc tggcggctgt tcaagaagat tagcggatcc gcgagtgcga
      241 aggaacttgc atgtcaggag atcacggttc cactgtgcaa aggtataggg tacaactata
      301 cttacatgcc taatcaattt aaccatgaca cccaagatga agcgggcctg gaggtccacc
      361 aattctggcc gcttgtggaa atccaatgta gcccggatct taagtttttc ttgtgttcca
      421 tgtatacgcc aatttgcctg gaggattata aaaagcccct cccgccttgc cgctcagttt
      481 gtgaacgggc gaaggctggg tgcgcacccc ttatgaggca atatggcttc gcgtggccag
      541 atcgcatgag atgtgatcgc ttgccggaac aagggaatcc tgatactctg tgtatggact
      601 ataacaggac ggatctcacc accgcggcac cgtcccctcc ccggaggttg cctcctccac
      661 caccgggcga gcagccccca agtggaagtg gccatgggag accgccggga gctaggcctc
      721 cacatcgcgg aggtggaagg gggtggtgga ggcg
//
