LOCUS       sequence-379774-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD9 with an N-terminal HiBiT; contains P199R; 
            I448V; S524N; R556H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD9
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aataggcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct tgccaccatg
      121 cggctctgca tcccgcaggt gctgttggcc ttgttccttt ccatgctgac agggccggga
      181 gaaggcagcc gggtgagcgg ctggcggctg ttcaagaaga ttagcggatc cctggagatc
      241 ggccgcttcg acccggagcg cgggcgcggg gctgcgccgt gccaggcggt ggagatcccc
      301 atgtgccgcg gcatcggcta caacctgacc cgcatgccca acctgctggg ccacacgtcg
      361 cagggcgagg cggctgccga gctagcggag ttcgcgccgc tggtgcagta cggctgccac
      421 agccacctgc gcttcttcct gtgctcgctc tacgcgccca tgtgcaccga ccaggtctcg
      481 acgcccattc ccgcctgccg gcccatgtgc gagcaggcgc gcctgcgctg cgcgcccatc
      541 atggagcagt tcaacttcgg ctggccggac tcgctcgact gcgcccggct gcccacgcgc
      601 aacgacccgc acgcgctgtg catggaggcg cccgagaacg ccacggccgg ccccgcggag
      661 ccccacaagg gcctgggcat gctgcccgtg gcgccgcggc ccgcgcgccc tcccggagac
      721 ctgggcccgg gcgcgggcgg cagtggcacc tgcgagaacc gcgagaagtt ccagtacgtg
      781 gagaagagcc gctcgtgcgc accgcgctgc gggcccggcg tcgaggtgtt ctggtcccgg
      841 cgcgacaagg acttcgcgct ggtctggatg gccgtgtggt cggcgctgtg cttcttctcc
      901 accgccttca ctgtgctcac cttcttgctg gagccccacc gcttccagta ccccgagcgc
      961 cccatcatct tcctctccat gtgctacaac gtctactcgc tggccttcct gatccgtgcg
     1021 gtggccgaaa cgcaaaacgg ggcctgtg
//
