LOCUS       sequence-379776-        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FZD10 with an N-terminal HiBiT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson
            G, Schulte G
  TITLE     Quantitative Profiling of WNT-3A Binding to All Human Frizzled 
            Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
  JOURNAL   ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 
            10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
  PUBMED    34151213
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsptsci.1c00084"; journalName: "ACS Pharmacol Transl Sci";
            date: "2021-05-11- 11"; volume: "4"; issue: "3"; pages: "1235-1245"
COMMENT     Sequence Label: HiBiT-FZD10
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataagcagag ctctctggct aactagagaa ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg ccaccatgcg
      121 gctctgcatc ccgcaggtgc tgttggcctt gttcctttcc atgctgacag ggccgggaga
      181 aggcagccgg gtgagcggct ggcggctgtt caagaagatt agcggatcca tcagctccat
      241 ggacatggag cgcccgggcg acggcaaatg ccagcccatc gagatcccga tgtgcaagga
      301 catcggctac aacatgactc gtatgcccaa cctgatgggc cacgagaacc agcgcgaggc
      361 agccatccag ttgcacgagt tcgcgccgct ggtggagtac ggctgccacg gccacctccg
      421 cttcttcctg tgctcgctgt acgcgccgat gtgcaccgag caggtctcta cccccatccc
      481 cgcctgccgg gtcatgtgcg agcaggcccg gctcaagtgc tccccgatta tggagcagtt
      541 caacttcaag tggcccgact ccctggactg ccggaaactc cccaacaaga acgaccccaa
      601 ctacctgtgc atggaggcgc ccaacaacgg ctcggacgag cccacccggg gctcgggcct
      661 gttcccgccg ctgttccggc cgcagcggcc ccacagcgcg caggagcacc cgctgaagga
      721 cgggggcccc gggcgcggcg gctgcgacaa cccgggcaag ttccaccacg tggagaagag
      781 cgcgtcgtgc gcgccgctct gcacgcccgg cgtggacgtg tactggagcc gcgaggacaa
      841 gcgcttcgca gtggtctggc tggccatctg ggcggtgctg tgcttcttct ccagcgcctt
      901 caccgtgctc accttcctca tcgacccggc ccgcttccgc taccccgagc gccccatcat
      961 cttcctctcc atgtgctact gcgtctactc cgtgggctac ctcatccgcc tcttcgccgg
     1021 cgccgagagc atcgcctgcg accgggacag cggccagctc tatgtcatcc aggaagggac
     1081 tggaagagca ccggctgcac gctggtcttc ctggtcctct actacttcgg catggccagc
     1141 tcgctgtggg tgggtggtcc tcacgctcac ctgggttcct ggccgccgga
//
