LOCUS       sequence-379784-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse SMO with an N-terminal HiBiT; contains D54N and 
            R690G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Turku A, Schihada H, Kozielewicz P, Bowin CF, Schulte G
  TITLE     Residue 6.43 defines receptor function in class F GPCRs.
  JOURNAL   Nat Commun. 2021 Jun 24;12(1):3919. doi: 10.1038/s41467-021-24004-z.
  PUBMED    34168128
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-24"; volume: "12(1):3919. doi"; pages: " 
            10.1038/s41467-021-24004-z"
COMMENT     Sequence Label: HiBiT-SMO
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcaatagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca
      121 tgcggctctg catcccgcag gtgctgttgg ccttgttcct ttccatgctg acagggccgg
      181 gagaaggcag ccgggtgagc ggctggcggc tgttcaagaa gattagcgga tccgcggcct
      241 tgagcgggaa cgtgaccggg cctgggcctc acagcgccag cgggagctcg aggaggaacg
      301 tgccggtgac cagccctccg ccgccgctgc tgagccactg cggccgggcc gcccactgcg
      361 agcctttgcg ctacaacgtg tgcctgggct cggcgctgcc ctacggagcc accaccacgc
      421 tgctggctgg ggactcggac tcgcaggagg aagcgcacgg caagctcgtg ctctggtccg
      481 gcctccggaa tgccccccgc tgctgggcag tgatccagcc cctgctgtgt gctgtctaca
      541 tgcccaagtg tgagaatgac cgagtggagt tgcccagccg taccctctgc caggccaccc
      601 gaggcccctg tgccattgtg gagcgggagc gagggtggcc tgactttctg cgttgcacac
      661 cggaccactt ccctgaaggc tgcccaaacg aggtacaaaa catcaagttc aacagctcag
      721 gccaatgtga agcacccttg gtgcgaacag acaaccccaa gagctggtat gaggacgtgg
      781 agggctgtgg gattcagtgt cagaacccgc tgttcaccga ggccgagcac caggacatgc
      841 acagctacat cgcagccttc ggggcggtca ctggtctctg cacgctcttc acactggcca
      901 cctttgtggc tgactggcgg aattccaatc gctaccctgc ggttattctc ttctatgtca
      961 atgcgtgttt cttcgtgggc agcattggct ggctggccca gttcatggat ggtgcccgcc
     1021 gagagattgt ttgccgagca gatggcacca tgagatttgg ggagcccacc tccagtgaga
     1081 ccctgtcctg tgtcatcatc tttgtcattg tgtactatgc cttgatggct ggaataat
//
