LOCUS       sequence-379795-       11186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for protein subcellular 
            localization in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-Cam1304-SubN
FEATURES             Location/Qualifiers
     source          1..11186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        184..529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(545..547,548..550,551..1261)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(545..547,548..550,551..1261)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(590..611)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(851..870)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1198..1219
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1443..1545
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1576..1881
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1760..1779
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      1964..2216
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2238..2262
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3563..4192
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4621..5694
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5760..5954
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6190..6212)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6298..6438
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6353..6372
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6453..6470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6624..7212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6704..6723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7299..8093)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8518..8542
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8620..8794
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8835..9860)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9927..10603)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    10794..10815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10830..10835,10836..10853,10854..10860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10868..10884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10873..10895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10892..10908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10892..10908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             10909..11136
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    10917..10973
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10977..10994)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10977..10993)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10986..11008)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
       61 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      121 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      181 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      241 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      301 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      421 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
      541 tgacatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      601 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      661 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      721 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      781 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      841 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      901 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      961 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1021 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1081 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1141 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1201 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1261 gcagtggtct ctgtccagtc ctggcctcgt cggccattct cgactaagtt ggcagcatca
     1321 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
     1381 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     1441 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1501 gtattttttg agttatcgag attttcagga gctaaggaag ctaaactttt gctgacgaga
     1561 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     1621 ctgtttgtgg atgtacagag tgatattatt gacacgcctg ggcgacggat ggtgatcccc
     1681 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     1741 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtatccgtt
     1801 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     1861 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagggccag tctggccact
     1921 tgtggtctgc tgagaccgtg aggtgaccag ctcgaatttc cccgatcgtt caaacatttg
     1981 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     2041 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     2101 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     2161 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     2221 attaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     2281 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     2341 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     2401 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     2461 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     2521 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     2581 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     2641 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     2701 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     2761 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     2821 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     2881 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     2941 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga
     3001 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     3061 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     3121 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     3181 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     3241 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     3301 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     3361 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     3421 cctggccggc gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     3481 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     3541 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     3601 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     3661 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     3721 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     3781 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     3841 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     3901 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     3961 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     4021 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     4081 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     4141 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     4201 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     4261 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     4321 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     4381 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     4441 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     4501 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     4561 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     4621 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     4681 gtggagaagt tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     4741 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     4801 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     4861 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     4921 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     4981 gggcacgtag aggtttccgc agggccggcg ggcatggcca gtgtgtggga ttacgacctg
     5041 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     5101 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     5161 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     5221 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     5281 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     5341 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     5401 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     5461 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     5521 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     5581 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     5641 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     5701 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     5761 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     5821 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     5881 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     5941 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     6001 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     6061 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     6121 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     6181 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6241 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     6301 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     6361 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     6421 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7261 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     7321 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     7381 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     7441 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     7501 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     7561 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     7621 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     7681 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     7741 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     7801 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     7861 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     7921 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     7981 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     8041 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     8101 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     8161 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     8221 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     8281 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     8341 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     8401 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     8461 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     8521 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     8581 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
     8641 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
     8701 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
     8761 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccc
     8821 ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
     8881 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
     8941 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
     9001 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
     9061 agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag
     9121 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
     9181 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
     9241 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
     9301 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
     9361 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
     9421 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
     9481 agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt
     9541 tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat gcaataggtc
     9601 aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
     9661 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
     9721 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
     9781 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
     9841 gtgagttcag gctttttcat atctcattgc cccccggatc tgcgaaagct cgagagagat
     9901 agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
     9961 tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
    10021 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    10081 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
    10141 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
    10201 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
    10261 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    10321 gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt
    10381 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    10441 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
    10501 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
    10561 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
    10621 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc
    10681 aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10741 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10801 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10861 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    10921 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcactg
    10981 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    11041 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    11101 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
    11161 gtcgtttccc gccttcagtt tagctt
//
