LOCUS       sequence-379796-       14265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for protein subcellular 
            localization in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 14265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-121-SubC
FEATURES             Location/Qualifiers
     source          1..14265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5749..5769
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        6002..6104
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6135..6440
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6319..6338
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             join(6498..6500,6501..6503,6504..7217)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6498..6500,6501..6503,6504..7214)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6543..6564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6804..6823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7151..7172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7234..7486
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7495..7512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7495..7511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7504..7526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7651..7670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7861..7882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8129..8153
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9749..10897)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11196..11990)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12182..12949
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(13642..14265,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gagtggtctc tgtccagtcc tggcctcgtc ggccattctc gactaagttg gcagcatcac
     5881 ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
     5941 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
     6001 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     6061 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaacttttg ctgacgagaa
     6121 cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     6181 tgtttgtgga tgtacagagt gatattattg acacgcctgg gcgacggatg gtgatccccc
     6241 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     6301 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtatccgtta
     6361 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     6421 tgatgttctg gggaatataa atgtcaggct cccttataca cagggccagt ctggccactt
     6481 gtggtctgct gagaccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6601 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6661 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     6721 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6781 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6901 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6961 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7081 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7141 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7201 acgagctgta caagtgagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     7261 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     7321 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     7381 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     7441 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attcactggc
     7501 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7561 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7621 ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct tcctttctcg
     7681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7741 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     7801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7861 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     7921 tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc tgctggggca
     7981 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     8041 gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac gtccgcaatg
     8101 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     8161 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     8221 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     8281 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     8341 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aaatatcatc
     8401 tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa ccgtgacagg
     8461 ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag ccgctgagga
     8521 agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc cctatccatt
     8581 gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg atgcatcccc
     8641 ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac tgtaggttcg
     8701 agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc
     8761 cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa
     8821 gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga
     8881 ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc
     8941 gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc
     9001 gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct
     9061 agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg
     9121 accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta
     9181 agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc
     9241 atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac ccctcggcct
     9301 cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc cttggggccg
     9361 tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc
     9421 acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa
     9481 acggacccga acatctctgg agctttcttc agggccgaca atcggatctc gcggaaatcc
     9541 tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt caattttaat
     9601 cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc
     9661 aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac
     9721 ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga
     9781 cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct
     9841 cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct
     9901 tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg
     9961 acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga
    10021 cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc
    10081 ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca
    10141 tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga
    10201 tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag
    10261 gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca
    10321 gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt
    10381 gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt
    10441 cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca
    10501 tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ttgcttggcc tcgctgacct
    10561 gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt
    10621 cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca
    10681 cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct
    10741 tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca
    10801 tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt
    10861 cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc
    10921 cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt
    10981 ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct
    11041 cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact
    11101 tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct
    11161 gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata
    11221 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
    11281 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    11341 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    11401 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    11461 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    11521 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    11581 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    11641 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    11701 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    11761 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    11821 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    11881 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    11941 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    12001 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    12061 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    12121 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat
    12181 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
    12241 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
    12301 aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc
    12361 agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga
    12421 atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac
    12481 gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt
    12541 catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg
    12601 ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg tattcggtga
    12661 acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat
    12721 atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc tgcacgtaat
    12781 cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc
    12841 acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat
    12901 cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc aattatgata
    12961 gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat gcaagttttt
    13021 atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt gccccctgaa
    13081 atccttacat acggcgatat cttctatata aaagatatat tatcttatca gtattgtcaa
    13141 tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg tagcttttta
    13201 aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca tacctcggta
    13261 taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga acacgagcac
    13321 ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc ccgccatgaa
    13381 gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc
    13441 actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc
    13501 gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg caatggcaag
    13561 gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
    13621 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
    13681 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
    13741 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
    13801 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
    13861 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
    13921 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
    13981 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
    14041 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
    14101 aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac
    14161 aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag
    14221 ggccatagac ggccgccagc ccagcggcga gggcaaccag cccgg
//
