LOCUS       sequence-379819-        8965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing 
            mouse super-Sox + Klf4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4
FEATURES             Location/Qualifiers
     source          1..8965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3882..3935
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5407..5995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5460..5480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5878..5889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6143..6323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6370..6388)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6456..6560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6561..6629,6630..7421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6779..6798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7592..8180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8334..8351
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8591..8612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8627..8632,8633..8650,8651..8657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8665..8681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8670..8692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8689..8705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8689..8705
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8755..8774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgtat aacatgatgg
     2701 agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc ggaggaggca
     2761 acgccacggc ggcggcaacc ggcggcaacc agaagaacag cccggaccgc gtcaagaggc
     2821 ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc caggagaacc
     2881 ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa gcgttgacct
     2941 tggcagagaa gcggccgttc atcgacgagg ccaagcggct gcgcgtgctg catatgcagg
     3001 accaccccaa ctacaagtac cggccgcggc ggcgcaagca ggtgaagcgc atgaagcggg
     3061 tggagggagg cttcctgcac gctctcgtcg agccccaggc cggcgcgctt ggtcccgagg
     3121 gcggccgcgt ggccatggat ggcctgggtc tgcctttccc ggagccgggc tatccggccg
     3181 gtcctccgct gatgtctccg cacatgggcc cccactatcg ggactgccag ggactgggcg
     3241 ctcccgcgct cgacggctac cctctgccca ctccggacac atccccgctg gatggcgtgg
     3301 agcaggaccc ggctttcttt gcagccccgc tgccagggga ctgcccggcg gccggcacct
     3361 acacttacgc tccagtctcg gactatgcag tgtccgtaga gccgcccgct ggccccatgc
     3421 gagtggggcc ggacccctcg ggccctgcga tgccggggat cctggcgccc cccagcgctc
     3481 tgcacctgta ctacggcgcg atgggctcgc ccgccgcaag tgcggggcgc ggtttccacg
     3541 cgcaacccca gcagccgctg caaccgcagg caccgccccc gccaccgcag cagcagcacc
     3601 cagcgcacgg ccccgggcaa ccttcgcccc ctcccgaggc tctgccctgc cgggatggca
     3661 cggaatccaa ccagcccact gagctcctag gggaggtgga ccgcacggaa tttgaacagt
     3721 atctgccctt tgtgtataag cccgagatgg gtcttcccta ccagggacac gactgcggag
     3781 tgaacctctc agacagccac ggagccattt cctccgtggt gtccgacgct agctcagcgg
     3841 tctactattg caactacccc gacattgcat gcggctccgg cgagggcagg ggaagtcttc
     3901 taacatgcgg ggacgtggag gaaaatcccg gcccactcga gatgaggcag ccacctggcg
     3961 agtctgacat ggctgtcagc gacgctctgc tcccgtcctt ctccacgttc gcgtccggcc
     4021 cggcgggaag ggagaagaca ctgcgtccag caggtgcccc gactaaccgt tggcgtgagg
     4081 aactctctca catgaagcga cttcccccac ttcccggccg cccctacgac ctggcggcga
     4141 cggtggccac agacctggag agtggcggag ctggtgcagc ttgcagcagt aacaacccgg
     4201 ccctcctagc ccggagggag accgaggagt tcaacgacct cctggaccta gactttatcc
     4261 tttccaactc gctaacccac caggaatcgg tggccgccac cgtgaccacc tcggcgtcag
     4321 cttcatcctc gtcttcccca gcgagcagcg gccctgccag cgcgccctcc acctgcagct
     4381 tcagctatcc gatccgggcc gggggtgacc cgggcgtggc tgccagcaac acaggtggag
     4441 ggctcctcta cagccgagaa tctgcgccac ctcccacggc ccccttcaac ctggcggaca
     4501 tcaatgacgt gagcccctcg ggcggcttcg tggctgagct cctgcggccg gagttggacc
     4561 cagtatacat tccgccacag cagcctcagc cgccaggtgg cgggctgatg ggcaagtttg
     4621 tgctgaaggc gtctctgacc acccctggca gcgagtacag cagcccttcg gtcatcagtg
     4681 ttagcaaagg aagcccagac ggcagccacc ccgtggtagt ggcgccctac agcggtggcc
     4741 cgccgcgcat gtgccccaag attaagcaag aggcggtccc gtcctgcacg gtcagccggt
     4801 ccctagaggc ccatttgagc gctggacccc agctcagcaa cggccaccgg cccaacacac
     4861 acgacttccc cctggggcgg cagctcccca ccaggactac ccctacactg agtcccgagg
     4921 aactgctgaa cagcagggac tgtcaccctg gcctgcctct tcccccagga ttccatcccc
     4981 atccggggcc caactaccct cctttcctgc cagaccagat gcagtcacaa gtcccctctc
     5041 tccattatca agagctcatg ccaccgggtt cctgcctgcc agaggagccc aagccaaaga
     5101 ggggaagaag gtcgtggccc cggaaaagaa cagccaccca cacttgtgac tatgcaggct
     5161 gtggcaaaac ctataccaag agttctcatc tcaaggcaca cctgcgaact cacacaggcg
     5221 agaaacctta ccactgtgac tgggacggct gtgggtggaa attcgcccgc tcggatgaac
     5281 tgaccaggca ctaccgcaaa cacacagggc accggccctt tcagtgccag aagtgcgaca
     5341 gggccttttc caggtcggac caccttgcct tacacatgaa gaggcacttt taaatcgata
     5401 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5461 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5521 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5581 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5641 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5701 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5761 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5821 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5881 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5941 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     6001 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6061 ggggggactg gaagggctaa ttcactccca acgaagacaa gatcacctgc aggacaggcg
     6121 cgccctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6181 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6241 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6301 gtcagtgtgg aaaatctcta gcacccgggc gattaaggaa agggctagat cattcttgaa
     6361 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6421 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6781 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     6841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7081 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7441 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7501 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8221 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8281 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8341 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8401 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
     8461 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8521 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8581 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8641 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8701 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8761 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     8821 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     8881 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     8941 aaatatgtac tacaaactta gtagt
//
