LOCUS       sequence-379823-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing 
            mouse super-Sox + Klf4 + cMyc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3882..3935
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5406..5465
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    6853..7441
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6906..6926)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7324..7335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7589..7769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7816..7834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7902..8006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8007..8075,8076..8867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8225..8244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9038..9626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9780..9797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10037..10058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10073..10078,10079..10096,10097..10103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10111..10127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10116..10138
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10135..10151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10135..10151
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10201..10220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgtat aacatgatgg
     2701 agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc ggaggaggca
     2761 acgccacggc ggcggcaacc ggcggcaacc agaagaacag cccggaccgc gtcaagaggc
     2821 ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc caggagaacc
     2881 ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa gcgttgacct
     2941 tggcagagaa gcggccgttc atcgacgagg ccaagcggct gcgcgtgctg catatgcagg
     3001 accaccccaa ctacaagtac cggccgcggc ggcgcaagca ggtgaagcgc atgaagcggg
     3061 tggagggagg cttcctgcac gctctcgtcg agccccaggc cggcgcgctt ggtcccgagg
     3121 gcggccgcgt ggccatggat ggcctgggtc tgcctttccc ggagccgggc tatccggccg
     3181 gtcctccgct gatgtctccg cacatgggcc cccactatcg ggactgccag ggactgggcg
     3241 ctcccgcgct cgacggctac cctctgccca ctccggacac atccccgctg gatggcgtgg
     3301 agcaggaccc ggctttcttt gcagccccgc tgccagggga ctgcccggcg gccggcacct
     3361 acacttacgc tccagtctcg gactatgcag tgtccgtaga gccgcccgct ggccccatgc
     3421 gagtggggcc ggacccctcg ggccctgcga tgccggggat cctggcgccc cccagcgctc
     3481 tgcacctgta ctacggcgcg atgggctcgc ccgccgcaag tgcggggcgc ggtttccacg
     3541 cgcaacccca gcagccgctg caaccgcagg caccgccccc gccaccgcag cagcagcacc
     3601 cagcgcacgg ccccgggcaa ccttcgcccc ctcccgaggc tctgccctgc cgggatggca
     3661 cggaatccaa ccagcccact gagctcctag gggaggtgga ccgcacggaa tttgaacagt
     3721 atctgccctt tgtgtataag cccgagatgg gtcttcccta ccagggacac gactgcggag
     3781 tgaacctctc agacagccac ggagccattt cctccgtggt gtccgacgct agctcagcgg
     3841 tctactattg caactacccc gacattgcat gcggctccgg cgagggcagg ggaagtcttc
     3901 taacatgcgg ggacgtggag gaaaatcccg gcccactcga gatgaggcag ccacctggcg
     3961 agtctgacat ggctgtcagc gacgctctgc tcccgtcctt ctccacgttc gcgtccggcc
     4021 cggcgggaag ggagaagaca ctgcgtccag caggtgcccc gactaaccgt tggcgtgagg
     4081 aactctctca catgaagcga cttcccccac ttcccggccg cccctacgac ctggcggcga
     4141 cggtggccac agacctggag agtggcggag ctggtgcagc ttgcagcagt aacaacccgg
     4201 ccctcctagc ccggagggag accgaggagt tcaacgacct cctggaccta gactttatcc
     4261 tttccaactc gctaacccac caggaatcgg tggccgccac cgtgaccacc tcggcgtcag
     4321 cttcatcctc gtcttcccca gcgagcagcg gccctgccag cgcgccctcc acctgcagct
     4381 tcagctatcc gatccgggcc gggggtgacc cgggcgtggc tgccagcaac acaggtggag
     4441 ggctcctcta cagccgagaa tctgcgccac ctcccacggc ccccttcaac ctggcggaca
     4501 tcaatgacgt gagcccctcg ggcggcttcg tggctgagct cctgcggccg gagttggacc
     4561 cagtatacat tccgccacag cagcctcagc cgccaggtgg cgggctgatg ggcaagtttg
     4621 tgctgaaggc gtctctgacc acccctggca gcgagtacag cagcccttcg gtcatcagtg
     4681 ttagcaaagg aagcccagac ggcagccacc ccgtggtagt ggcgccctac agcggtggcc
     4741 cgccgcgcat gtgccccaag attaagcaag aggcggtccc gtcctgcacg gtcagccggt
     4801 ccctagaggc ccatttgagc gctggacccc agctcagcaa cggccaccgg cccaacacac
     4861 acgacttccc cctggggcgg cagctcccca ccaggactac ccctacactg agtcccgagg
     4921 aactgctgaa cagcagggac tgtcaccctg gcctgcctct tcccccagga ttccatcccc
     4981 atccggggcc caactaccct cctttcctgc cagaccagat gcagtcacaa gtcccctctc
     5041 tccattatca agagctcatg ccaccgggtt cctgcctgcc agaggagccc aagccaaaga
     5101 ggggaagaag gtcgtggccc cggaaaagaa cagccaccca cacttgtgac tatgcaggct
     5161 gtggcaaaac ctataccaag agttctcatc tcaaggcaca cctgcgaact cacacaggcg
     5221 agaaacctta ccactgtgac tgggacggct gtgggtggaa attcgcccgc tccgatgaac
     5281 tgaccaggca ctaccgcaaa cacacagggc accggccctt tcagtgccag aagtgcgaca
     5341 gggccttttc caggtcggac caccttgcct tacacatgaa gaggcacttt ctcgagggct
     5401 cgggccagtg tactaattat gctctcttga aattggctgg agatgttgag agcaacccag
     5461 gtcccggcgc gccactggat ttcctttggg cgttggaaac cccgcagaca gccacgacga
     5521 tgcccctcaa cgtgaacttc accaacagga actatgacct cgactacgac tccgtacagc
     5581 cctatttcat ctgcgacgag gaagagaatt tctatcacca gcaacagcag agcgagctgc
     5641 agccgcccgc gcccagtgag gatatctgga agaaattcga gctgcttccc accccgcccc
     5701 tgtccccgag ccgccgctcc gggctctgct ctccatccta tgttgcggtc gctacgtcct
     5761 tctccccaag ggaagacgat gacggcggcg gtggcaactt ctccaccgcc gatcagctgg
     5821 agatgatgac cgagttactt ggaggagaca tggtgaacca gagcttcatc tgcgatcctg
     5881 acgacgagac cttcatcaag aacatcatca tccaggactg tatgtggagc ggtttctcag
     5941 ccgctgccaa gctggtctcg gagaagctgg cctcctacca ggctgcgcgc aaagacagca
     6001 ccagcctgag ccccgcccgc gggcacagcg tctgctccac ctccagcctg tacctgcagg
     6061 acctcaccgc cgccgcgtcc gagtgcattg acccctcagt ggtctttccc tacccgctca
     6121 acgacagcag ctcgcccaaa tcctgtacct cgtccgattc cacggccttc tctccttcct
     6181 cggactcgct gctgtcctcc gagtcctccc cacgggccag ccctgagccc ctagtgctgc
     6241 atgaggagac accgcccacc accagcagcg actctgaaga agagcaagaa gatgaggaag
     6301 aaattgatgt ggtgtctgtg gagaagaggc aaacccctgc caagaggtcg gagtcgggct
     6361 catctccatc ccgaggccac agcaaacctc cgcacagccc actggtcctc aagaggtgcc
     6421 acgtctccac tcaccagcac aactacgccg cacccccctc cacaaggaag gactatccag
     6481 ctgccaagag ggccaagttg gacagtggca gggtcctgaa gcagatcagc aacaaccgca
     6541 agtgctccag ccccaggtcc tcagacacgg aggaaaacga caagaggcgg acacacaacg
     6601 tcttggaacg tcagaggagg aacgagctga agcgcagctt ttttgccctg cgtgaccaga
     6661 tccctgaatt ggaaaacaac gaaaaggccc ccaaggtagt gatcctcaaa aaagccaccg
     6721 cctacatcct gtccattcaa gcagacgagc acaagctcac ctctgaaaag gacttattga
     6781 ggaaacgacg agaacagttg aaacacaaac tcgaacagct tcgaaactct ggtgcataaa
     6841 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6901 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6961 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7021 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7081 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7141 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7201 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7261 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7321 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7381 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7441 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7501 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc acctgcagga
     7561 caggcgcgcc ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7621 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7681 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7741 cttttagtca gtgtggaaaa tctctagcac ccgggcgatt aaggaaaggg ctagatcatt
     7801 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7861 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8161 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8221 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     8281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8521 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8581 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8641 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8701 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8761 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8821 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8881 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8941 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9841 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     9901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9961 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    10021 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10081 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10141 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    10201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10261 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10321 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10381 tttgttaaat atgtactaca aacttagtag t
//
