LOCUS       sequence-379847-       11394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 MoClo plasmid containing an arabinose inducible dCas9 for 
            CRISPRi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB1-A-pBAD-dCas9
FEATURES             Location/Qualifiers
     source          1..11394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..894)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        921..1205
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1128..1147
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             join(1237..1263,1264..1266,1267..3753,3754..3756,
                     3757..5343)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEENPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      5356..5383
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(9342..9359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9513..10101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9593..9612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10272..11063,11064..11132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10895..10914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11133..11237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11305..11323
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(11327..11370)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    11373..11392
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tctatttatg acaacttgac ggctacatca ttcacttttt cttcacaacc
       61 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
      121 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
      181 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
      241 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
      301 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
      361 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
      421 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
      481 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
      541 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
      601 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
      661 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
      721 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
      781 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
      841 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
      901 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
      961 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
     1021 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     1081 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     1141 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     1201 tccatgtact ttaactttaa gaaggagata tacataatgg ataagaaata ctcaataggc
     1261 ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
     1321 tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata
     1381 ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtcttaa acggacagct
     1441 cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat
     1501 gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt tttggtggaa
     1561 gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga agttgcttat
     1621 catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc tactgataaa
     1681 gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg tggtcatttt
     1741 ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt tatccagttg
     1801 gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg agtagatgct
     1861 aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct cattgctcag
     1921 ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc attgggtttg
     1981 acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca gctttcaaaa
     2041 gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca atatgctgat
     2101 ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat cctaagagta
     2161 aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta cgatgaacat
     2221 catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga aaagtataaa
     2281 gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg gggagctagc
     2341 caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg tactgaggaa
     2401 ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt tgacaacggc
     2461 tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag acaagaagac
     2521 ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac ttttcgaatt
     2581 ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag
     2641 tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg tgcttcagct
     2701 caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga aaaagtacta
     2761 ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac aaaggtcaaa
     2821 tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa gaaagccatt
     2881 gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa agaagattat
     2941 ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga tagatttaat
     3001 gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga ttttttggat
     3061 aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt atttgaagat
     3121 agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga taaggtgatg
     3181 aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa attgattaat
     3241 ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc agatggtttt
     3301 gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa agaagacatt
     3361 caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc aaatttagct
     3421 ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga tgaattggtc
     3481 aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg tgaaaatcag
     3541 acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga agaaggtatc
     3601 aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca attgcaaaat
     3661 gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga ccaagaatta
     3721 gatattaatc gtttaagtga ttatgatgtc gatgccattg ttccacaaag tttccttaaa
     3781 gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg taaatcggat
     3841 aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca acttctaaac
     3901 gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg tggaggtttg
     3961 agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact
     4021 aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga aaatgataaa
     4081 cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga cttccgaaaa
     4141 gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca tgatgcgtat
     4201 ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga atcggagttt
     4261 gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa
     4321 ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt cttcaaaaca
     4381 gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac taatggggaa
     4441 actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa agtattgtcc
     4501 atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt ctccaaggag
     4561 tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga ctgggatcca
     4621 aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt ggttgctaag
     4681 gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg gatcacaatt
     4741 atggaaagaa gttcctttga agaaaatccg attgactttt tagaagctaa aggatataag
     4801 gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga gttagaaaac
     4861 ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga gctggctctg
     4921 ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt gaagggtagt
     4981 ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta tttagatgag
     5041 attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc caatttagat
     5101 aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca agcagaaaat
     5161 attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa atattttgat
     5221 acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc cactcttatc
     5281 catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct aggaggtgac
     5341 taactcgatc acactggctc accttcgggt gggcctttct gcgtttatat gtttgccaga
     5401 gacgctgcag ttgcaagaca atatcgaccg cttgcagctt gataaagtga tcaaagaggg
     5461 acgagcagct acagcactgc taagggagat cggagagcca ctgccgccac cagtgaaagt
     5521 gaaaacaggt tggtttagtg ctgattatta cggctttgag gacatgctca cacgttggga
     5581 taatgatcaa tctagccttt ccatttatca cagagagata aaagaggagc aacaggaaca
     5641 gcaacgaaaa caacagcaga tagaaagaga aactaaacaa aaacaggaag atgacaaaaa
     5701 gaaacggcag gactatgaaa gatatttatc aaaatacggc tatgtcaaag atgctgagta
     5761 tgaccatatc agcgaccatg ttgcttcaga catttccatg tttacacgtc ttcaggcgca
     5821 ggcatgggca agtaacgata tgcaagagta cataaggtgt tcaaagcaaa aatacgagca
     5881 gatcagcgac agaatagcct atgagcgtga cttgaaaaaa atcgggcaat tgagccgtat
     5941 cctggataaa gacagacagg cacttgctga tattttaccg cagttgatgc cttactatga
     6001 gcagatgcaa caggctgtta ataaacggaa aattttactt gagaatgagc gacagccatc
     6061 atttaaacca cgttatgacc aagaacagcc taagccgaaa aaagacaatg acctaacttt
     6121 ttaatgtgtt acaatagcat tcaaatgtaa cacattggag tgaaaaacat gacagaagcg
     6181 acttttacct ttcgggtaga ccatgaccta aagcaagaat tttcaagcct tgctaagact
     6241 gttgatcggt cgggggcgca gctaatccgt gacttcatgc gtgactttgt taagaagcaa
     6301 caggaagccg cagactatga caaatggttc aagcagcagg tacagatcgg cttaaatgaa
     6361 gccaatgcag gcaagttgat tccacatgag gacgtaaagg cagagtttgc cgctagacgt
     6421 gctgcaacat tggcgaaact ggcagcgaag aatgaagatt gagtggactg aaacagctcg
     6481 ccaagatcgt agaaatatct atgattatct tgaagaacgc aaccctatag cagctattga
     6541 aattgatgat ttaattgaag aaaagacaga tttacttgtt gataatcgac tgatggggcg
     6601 cacaggcaga cagaaagata ctagggagtt agtgatacat ccgcattatg tggttgtata
     6661 tgacatcact gatataatac ggatactcag agtgctacac acatcgcagg agtggtcatg
     6721 acttactcat gtactttgga ttatttagtg ttataaaatc ctgatttata aatttttttt
     6781 gttaaaaaag ataaaagccc cttgcaattg cttggggctt taccgtaatt tatggggtac
     6841 agatcttcga tactgacata tcggcaatcg aaagcattaa ggtttgacga ccgctaatga
     6901 tttcaccaca ggggcttaat gtacctgtct taaattctaa ggttttaact cgctttgtca
     6961 agcatagacc ccaaaaattt agccaatgtc tgtaactcaa tctgtccatg tgtgggtgat
     7021 gaggtacagt gacgctagca cacatcggaa aaacgctatt actaggggaa ctgaacagag
     7081 tagcggacgc aatgagtagt catttaattg gcggttatga gcgtgttcag gcggtgctat
     7141 caatcgtaat cataacagtg gcagcttgat acagtgatgt catccctgat gcgaaagcga
     7201 ccgaccgacg gtacatcgaa tgggaatact ttagggtgat ttttaagaat cgctctaggg
     7261 tgagtatttc ccattcagct ctgctccctc cctctggtac tttaatcaaa agcactacta
     7321 aacatatgtt tttaaataaa aaatattgat atagagataa tattagtaag aataattaaa
     7381 caattgaata tagataaatc attgttaaat aaagattaat tattaaaatg aatgtatact
     7441 tatatataaa tcaatgattt aaaatatttg ataaagaaaa cttttcaaaa aaaatataat
     7501 tgagattgtg tcatttcggt caattcttaa tatgttccac gcaagtttta gctatggtgc
     7561 taaacagaaa tttgctgaaa aagaactttt cactgaactg gttaaaatgt aagcagcctg
     7621 agagccgcca aaaattttaa aaacaaaccg ccttaatcat cttcaaaaaa tacctctaaa
     7681 acctcaccat ttgcgtttta agacccatat ttcatcctgc ccttatgttc ccatgctgat
     7741 agctataaag tgtctgtaat cgcttcctat gacgttctag gctgttgata acttttggaa
     7801 caacgcaaaa tgttaaaatc cgatcatttt ttaacctagt tattttcgtt acagggtaac
     7861 tttggtagta ttatttcaat atttagttgt tacaggataa caaaatatat gttacagggt
     7921 aattaattta gcgttacagg ataactatag ttacagggta acgttaaata gttatcctgt
     7981 aatttaaaaa tagttgcagg atagcataaa tttttacaaa taaattttat aaaaagttat
     8041 cctgtaacta aaaacaatgt tatcctgtaa ctatgatata ctatatggga gttaaagaca
     8101 tgaaagaccc gaacgatcag aaaacacagg acatgctaaa agagccgcca aaaaccaatg
     8161 ccgaacgaca aaaagcatat cgtgaaaaac gcaaaagcct tgatagtcaa cgtttggagg
     8221 tgtttataga taagggtgtg tcggatatgc ttgcggacat ggtgggcgca gcaggggaga
     8281 gccaaaaagc cattttgacc gcattgattg agaaagagta taagcggctg tatgcagtga
     8341 aaaatagcaa gctaaaaaag tacattgatt caaaaagtaa tgcagataaa aaagaaaccc
     8401 tcgatttatg agggtttttt ataactacta atgcctatga aaataagtgc ttatcatttt
     8461 ctctaatgta cccaataaaa tcggggtttt gttccacaag tttcttgatg tcattatcat
     8521 gtttcttcag taagtaagcc ccgatcagga tcttgcgttt tgcatcgtct gaccttgcct
     8581 gtttttgctc tcgacctttc accacagcgt cagctagatc ctttcttgcc ttttcctgac
     8641 gttccaattt ttccttttct tcatcgagca tttgacccaa ttccatagct gtttttttga
     8701 ccatttttaa tgcctgtgtc tcaacctatt ttccgaagta tgaaccatca tcaatgtgaa
     8761 aagtgttcta cttcaaaaac tcgacttaca tcaagatccg agcgcagcga gcaaaatcaa
     8821 aaaacaaagt caaagcctta ttgctcttgc ttttgctttt agcctcgcag agttcccgaa
     8881 gggcgcactt acgcaaaatt tttgctacgc caaattttgc aagtacggtc agggaaaccc
     8941 cgacacccca accgcccaaa acttggggcg gctgttaata aacaggtgat gaaaatggca
     9001 atttaccatt gtgaaatgca gaacatttcg aggtcagatg gtcgctcaat cgtggcatgt
     9061 gcagcatacc gagcaggcga aaaattgtac tgtgatacgt acggaaaaga gcaggactac
     9121 accaaaaaaa caggcattga atacacccaa atttttgccc caactggggc aagtcctgac
     9181 atgttagatc gtcaaaccct atggaatcgg gtagagcaat ccgaactaaa aaagaacggt
     9241 gacatcaaac aggaggcaag attagcaaag gaagtagaga tagcattgcc gcatgaactg
     9301 gataagacac agcgtcaggc aacttgttaa ctgcaggctt cctcgctcac tgactcgctg
     9361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9481 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10381 ccatctggcc ccagtgctgc aatgataccg cgagagccac gctcaccggc tccagattta
    10441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10561 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    11281 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
    11341 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agaa
//
