LOCUS       sequence-379848-       17064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4
            + super-SOX + GLIS1 + PuroR) for generation of integration-free 
            iPSCs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17064)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 17064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1
FEATURES             Location/Qualifiers
     source          1..17064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1535..1543
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      7586..7595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8678..8731
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10154..10213
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             11090..11107
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             11096..11113
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     misc_feature    11459..12008
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      12016..12025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    13953..14502
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             14505..15104
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(14505..14524)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     15001..15021
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        15302..15406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15407..15475,15476..16267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15625..15644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16438..17026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16927..16946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaggcagacg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
     7561 gtctagcata tgggcgcgtg aattcgccac catggcggga cacctggctt cggatttcgc
     7621 cttctcgccc cctccaggtg gtggaggtga tgggccaggg gggccggagc cgggctgggt
     7681 tgatcctcgg acctggctaa gcttccaagg ccctcctgga gggccaggaa tcgggccggg
     7741 ggttgggcca ggctctgagg tgtgggggat tcccccatgc cccccgccgt atgagttctg
     7801 tggggggatg gcgtactgtg ggccccaggt tggagtgggg ctagtgcccc aaggcggctt
     7861 ggagacctct cagcctgagg gcgaagcagg agtcggggtg gagagcaact ccgatggggc
     7921 ctccccggag ccctgcaccg tcacccctgg tgccgtgaag ctggagaagg agaagctgga
     7981 gcaaaacccg gaggagtccc aggacatcaa agctctgcag aaagaactcg agcaatttgc
     8041 caagctcctg aagcagaaga ggatcaccct gggatataca caggccgatg tggggctcac
     8101 cctgggggtt ctatttggga aggtattcag ccaaacgacc atctgccgct ttgaggctct
     8161 gcagcttagc ttcaagaaca tgtgtaagct gcggcccttg ctgcagaagt gggtggagga
     8221 agctgacaac aatgaaaatc ttcaggagat atgcaaagca gaaaccctcg tgcaggcccg
     8281 aaagagaaag cgaaccagta tcgagaaccg agtgagaggc aacctggaga atttgttcct
     8341 gcagtgcccg aaacccacac tgcagcagat cagccacatc gcccagcagc ttgggctcga
     8401 gaaggatgtg gtccgagtgt ggttctgtaa ccggcgccag aagggcaagc gatcaagcag
     8461 cgactatgca caacgagagg attttgaggc tgctgggtct cctttctcag ggggaccagt
     8521 gtcctttcct ctggccccag ggccccattt tggtacccca ggctatggga gccctcactt
     8581 cactgcactg tactcctcgg tccctttccc tgagggggaa gcctttcccc ctgtctccgt
     8641 caccactctg ggctctccca tgcattcaaa ctctagtgag ggcagaggaa gtctgctaac
     8701 atgcggtgac gtcgaggaga atcctggccc acaattgatg gctgtcagcg acgcgctgct
     8761 cccatctttc tccacgttcg cgtctggccc ggcgggaagg gagaagacac tgcgtcaagc
     8821 aggtgccccg aataaccgct ggcgggagga gctctcccac atgaagcgac ttcccccagt
     8881 gcttcccggc cgcccctatg acctggcggc ggcgaccgtg gccacagacc tggagagcgg
     8941 cggagccggt gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga gagagaccga
     9001 ggagttcaac gatctcctgg acctggactt tattctctcc aattcgctga cccatcctcc
     9061 ggagtcagtg gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt cgtcgccgtc
     9121 gagcagcggc cctgccagcg cgccctccac ctgcagcttc acctatccga tccgggccgg
     9181 gaacgacccg ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg gcagggagtc
     9241 cgctccccct ccgacggctc ccttcaacct ggcggacatc aacgacgtga gcccctcggg
     9301 cggcttcgtg gccgagctcc tgcggccaga attggacccg gtgtacattc cgccgcagca
     9361 gccgcagccg ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt cgctgagcgc
     9421 ccctggcagc gagtacggca gcccgtcggt catcagcgtc agcaaaggca gccctgacgg
     9481 cagccacccg gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt gccccaagat
     9541 caagcaggag gcggtctctt cgtgcaccca cttgggcgct ggaccccctc tcagcaatgg
     9601 ccaccggccg gctgcacacg acttccccct ggggcggcag ctccccagca ggactacccc
     9661 gaccctgggt cttgaggaag tgctgagcag cagggactgt caccctgccc tgccgcttcc
     9721 tcccggcttc catccccacc cggggcccaa ttacccatcc ttcctgcccg atcagatgca
     9781 gccgcaagtc ccgccgctcc attaccaaga gctcatgcca cccggttcct gcatgccaga
     9841 ggagcccaag ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg ccacccacac
     9901 ttgtgattac gcgggctgcg gcaaaaccta cacaaagagt tcccatctca aggcacacct
     9961 gcgaacccac acaggtgaga aaccttacca ctgtgactgg gacggctgtg gatggaaatt
    10021 cgcccgctca gatgaactga ccaggcacta ccgtaaacac acggggcacc gcccgttcca
    10081 gtgccaaaaa tgcgaccgag cattttccag gtcggaccac ctcgccttac acatgaagag
    10141 gcatttttct agacaatgta ctaactacgc tttgttgaaa ctcgctggcg atgttgaaag
    10201 taaccccggt cctggcgcgc ccatgtacaa catgatggag acggagctga agccgccggg
    10261 cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg ccggcggcaa
    10321 ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg
    10381 cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa
    10441 gcgcctgggc gccgagtgga aagcgctgac gctggcggag aagcggccgt tcatcgacga
    10501 ggctaagcgg ctgcgagtgc tgcacatgca ggaccacccc aactacaagt accggccgcg
    10561 gcggcgcaag caggtgaagc ggctgaagcg ggtggagggc ggcttcctgc acggcctggc
    10621 tgagccgcag gcggccgcgc tgggccccga gggcggccgc gtggccatgg acggcctggg
    10681 cctccagttc cccgagcagg gcttccccgc cggcccgccg ctgctgcctc cgcacatggg
    10741 cggccactac cgcgactgcc agagtctggg cgcgcctccg ctcgacggct acccgttgcc
    10801 cacgcccgac acgtccccgc tggacggcgt ggaccccgac ccggctttct tcgccgcccc
    10861 gatgcccggg gactgcccgg cggccggcac ctacagctac gcgcaggtct cggactacgc
    10921 tggccccccg gagcctcccg ccggtcccat gcacccccga ctcggcccag agcccgcggg
    10981 tccctcgatt ccgggcctcc tggcgccacc cagcgccctt cacgtgtact acggcgcgat
    11041 gggctcgccc ggggcgggcg gcgggcgcgg cttccagatg cagccgcaac accagcacca
    11101 gcaccagcac cagcaccacc ccccgggccc cggacagccg tcgccccctc cggaggcact
    11161 gccctgccgg gacggcacgg accccagtca gcccgccgag ctcctcgggg aggtggaccg
    11221 cacggaattt gaacagtatc tgcacttcgt gtgcaagcct gagatgggcc tcccctacca
    11281 ggggcatgac tccggtgtga atctccccga cagccacggg gccatttcct cggtggtgtc
    11341 cgacgccagc tccgcggtat attactgcaa ctatcctgac gtgtgagcgg ccatcgataa
    11401 ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac
    11461 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
    11521 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
    11581 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
    11641 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
    11701 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
    11761 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
    11821 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
    11881 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
    11941 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
    12001 cgataatacg aattcgccac catggcagag gcccgcacat ccctgtctgc ccactgtcgg
    12061 ggcccgctgg ccactggcct gcacccagac ctggacctcc cgggccgaag cctcgccacc
    12121 cctgcgcctt cctgctacct tctgggcagc gaacccagct ctggcctggg cctccagccc
    12181 gagacccacc tccccgaggg cagcctgaag cggtgctgcg tcttgggcct accccccacc
    12241 tccccagcct cctcctcacc ctgtgcctcc tccgacgtca cctccatcat ccgctcctcc
    12301 cagacgtctc tggtcacctg tgtaaatgga ctccggagcc cccctctgac gggagatctg
    12361 gggggccctt ccaagcgggc ccggcctggc cctgcatcga cggacagcca tgagggcagc
    12421 ttgcaacttg aagcctgccg gaaggcgagc ttcctgaagc aggaacccgc ggatgagttt
    12481 tcagagctct ttgggcctca ccagcagggc ctgccgcccc cctatcccct gtctcagttg
    12541 ccgcctggcc caagccttgg aggcctgggg ctgggcctgg caggcagggt ggtggccggg
    12601 cggcaggcgt gccgctgggt ggactgctgt gcagcctatg agcagcagga ggagctggtg
    12661 cggcacatcg agaagagcca catcgaccag cgcaagggcg aggacttcac ctgcttctgg
    12721 gctggctgcg tgcgccgcta caagcccttc aacgcccgct acaagctgct catccacatg
    12781 cgagtgcact cgggcgagaa gcccaacaag tgcatgtttg aaggctgcag caaggccttc
    12841 tcacggctgg agaacctcaa gatccacctg aggagccaca cgggcgagaa gccgtacctg
    12901 tgccagcacc cgggttgcca gaaggccttc agcaactcca gcgaccgcgc caagcaccag
    12961 cgcacccacc tagacacgaa gccgtacgcc tgtcagatcc ctggctgctc caagcgctac
    13021 acagacccca gctccctccg caagcacgtc aaggcccatt cagccaaaga gcagcaggtg
    13081 cgtaagaagc tgcatgcggg ccctgacacc gaggccgacg tcctgaccga gtgtctggtc
    13141 ctgcagcagc tccacacgtc cacacagctg gctgccagcg acggcaaggg tggctgtggc
    13201 ctgggccagg agctgctccc aggtgtgtat cctggctcca tcacccccca taacggactt
    13261 gcatcgggcc tcctgccccc agcgcacgac gtaccttcca ggcaccaccc gctggatgcc
    13321 accaccagtt cccaccacca tctgtcccct ctgcccatgg ctgagagcac ccgggatggg
    13381 ttggggcccg gcctcctctc accaatagtc agccccctga aggggctggg gccaccgccg
    13441 ctgcccccat cctctcagag ccattctccg gggggccagc ccttccccac actccccagc
    13501 aagccgtcct acccaccctt ccagagccct ccacccccgc ctctgcccag cccacaaggt
    13561 taccagggca gtttccactc catccagagt tgcttcccct atggcgactg ctaccggatg
    13621 gctgaaccag cagccggtgg ggacggactg gtcggggaga cccacggttt caaccccctg
    13681 cggcccaatg gctaccacag cctcagcacg cccttgcctg ccacaggcta tgaggccctg
    13741 gctgaggcct catgccccac agcgctgcca cagcagccat ctgaagatgt ggtgtccagc
    13801 ggccccgagg actgtggctt cttccccaat ggagcctttg accactgcct gggccacatc
    13861 ccctccatct acacagacac ctgagcggcc gcaactaact taagctagca acggtttccc
    13921 tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata
    13981 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
    14041 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
    14101 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
    14161 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
    14221 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
    14281 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
    14341 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
    14401 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
    14461 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatgacc gagtacaagc
    14521 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    14581 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
    14641 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    14701 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    14761 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    14821 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    14881 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    14941 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    15001 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    15061 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagaattg gcaagctgct
    15121 tacatagaac tcgcggcgat tggcatgccg ccttaaaatt tttattttat tttttctttt
    15181 cttttccgaa tcggattttg tttttaatat ttcaaaaaaa aaaaaaaaaa aaaaaaaacg
    15241 cgtcgagggg aattaattct tgaagacgaa agggccaggt ggcacttttc ggggaaatgt
    15301 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    15361 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
    15421 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    15481 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
    15541 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
    15601 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
    15661 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
    15721 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
    15781 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
    15841 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
    15901 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
    15961 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
    16021 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    16081 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    16141 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    16201 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    16261 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    16321 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    16381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    16441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    16501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    16561 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
    16621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    16681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    16741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    16801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    16861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    16921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    16981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    17041 gagctctaat acgactcact atag
//
