LOCUS       sequence-379856-       11488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-replicating RNA vector expressing mCherry fluorescent protein +
            PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: T7-VEE-mCherry
FEATURES             Location/Qualifiers
     source          1..11488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1535..1543
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7600..8310
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7745..7763)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8013..8032
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8377..8926
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8521..8538)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8748..8767
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8929..9528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8929..8948)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9425..9445
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        9726..9830
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9831..9899,9900..10691)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10049..10068)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10862..11450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11351..11370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaggcagacg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
     7561 gtctagcata tgggcgcgtg aattcgccac cgcggccgca tggtgagcaa gggcgaggag
     7621 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     7681 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     7741 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     7801 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     7861 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     7921 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     7981 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     8041 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     8101 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     8161 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     8221 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     8281 accggcggca tggacgagct gtacaagtaa atcgataact aacttaagct agcaacggtt
     8341 tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     8401 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     8461 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     8521 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     8581 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     8641 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     8701 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     8761 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     8821 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     8881 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac
     8941 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     9001 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9061 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9121 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9181 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9241 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9301 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9361 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9421 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9481 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attggcaagc
     9541 tgcttacata gaactcgcgg cgattggcat gccgccttaa aatttttatt ttattttttc
     9601 ttttcttttc cgaatcggat tttgttttta atatttcaaa aaaaaaaaaa aaaaaaaaaa
     9661 aacgcgtcga ggggaattaa ttcttgaaga cgaaagggcc aggtggcact tttcggggaa
     9721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9781 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9841 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9901 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9961 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10021 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
    10081 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10141 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10201 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10261 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10321 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10381 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10441 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10501 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10561 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10621 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10681 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10741 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10801 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10861 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10921 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10981 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    11041 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11101 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11161 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11221 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11281 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11341 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11401 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11461 acgcgagctc taatacgact cactatag
//
