LOCUS       sequence-379878-        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for IGFBP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Conway JRW, Dinc DD, Follain G, Paavolainen O, Kaivola J, Bostrom P,
            Hartiala P, Peuhu E, Ivaska J
  TITLE     IGFBP2 secretion by mammary adipocytes limits breast cancer 
            invasion.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg1840. doi: 10.1126/sciadv.adg1840. 
            Epub 2023 Jul 12.
  PUBMED    37436978
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg1840. doi"; pages: " 
            10.1126/sciadv.adg1840. Epub 2023 Jul 12"
COMMENT     Sequence Label: pLenti6.3/TO/V5-DEST-IGFBP2
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             253..433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    480..605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1098..1331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1516..1560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1827..1944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2270..2473
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2424..2444
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2475..2493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2496..2514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2555..2579
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3573..3597)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3650..3691
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3715..4303
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3768..3788)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4186..4197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4318..4634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4485..4620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4682..4729
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4748..5146
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5233..5466
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5538..5659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5575..5594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5629..5648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5699..5834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5854..5873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5855..5873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5883..5900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5883..5899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5892..5914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6041..6496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6128..6147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6338..6359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6522..6626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6627..6695,6696..7487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6845..6864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7658..8246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8147..8166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8400..8417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8534..8555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8570..8575,8576..8593,8594..8600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8608..8624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8613..8635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8632..8648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8632..8648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8667..8687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8669..8687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
       61 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      121 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      181 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      241 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      301 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      361 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      421 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      481 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      541 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      601 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      661 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      721 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      781 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      841 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
      901 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
      961 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1021 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1081 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1141 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1201 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1261 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1321 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1381 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1441 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1501 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1561 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1621 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1681 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     1741 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     1801 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     1861 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1921 caaattacaa aaattcaaaa ttttatcgat aagcttggga gttccgctta cataacttac
     1981 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2041 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2101 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2161 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2221 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2281 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2341 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2401 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2461 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2521 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc
     2581 caccatgctg ccgagagtgg gctgccccgc gctgccgctg ccgccgccgc cgctgctgcc
     2641 gctgctgccg ctgctgctgc tgctactggg cgcgagtggc ggcggcggcg gggcgcgcgc
     2701 ggaggtgctg ttccgctgcc cgccctgcac acccgagcgc ctggccgcct gcgggccccc
     2761 gccggttgcg ccgcccgccg cggtggccgc agtggccgga ggcgcccgca tgccatgcgc
     2821 ggagctcgtc cgggagccgg gctgcggctg ctgctcggtg tgcgcccggc tggagggcga
     2881 ggcgtgcggc gtctacaccc cgcgctgcgg ccaggggctg cgctgctatc cccacccggg
     2941 ctccgagctg cccctgcagg cgctggtcat gggcgagggc acttgtgaga agcgccggga
     3001 cgccgagtat ggcgccagcc cggagcaggt tgcagacaat ggcgatgacc actcagaagg
     3061 aggcctggtg gagaaccacg tggacagcac catgaacatg ttgggcgggg gaggcagtgc
     3121 tggccggaag cccctcaagt cgggtatgaa ggagctggcc gtgttccggg agaaggtcac
     3181 tgagcagcac cggcagatgg gcaagggtgg caagcatcac cttggcctgg aggagcccaa
     3241 gaagctgcga ccaccccctg ccaggactcc ctgccaacag gaactggacc aggtcctgga
     3301 gcggatctcc accatgcgcc ttccggatga gcggggccct ctggagcacc tctactccct
     3361 gcacatcccc aactgtgaca agcatggcct gtacaacctc aaacagtgca agatgtctct
     3421 gaacgggcag cgtggggagt gctggtgtgt gaaccccaac accgggaagc tgatccaggg
     3481 agcccccacc atccgggggg accccgagtg tcatctcttc tacaatgagc agcaggaggc
     3541 tcgcggggtg cacacccagc ggatgcagta ggacccagct ttcttgtaca aagtggttga
     3601 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     3661 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgat cgacaatcaa
     3721 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3781 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3841 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3901 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3961 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4021 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4081 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4141 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4201 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4261 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggcga tggtaccggt
     4321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4381 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4441 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4501 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4561 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4621 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     4681 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     4741 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     4801 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     4861 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     4921 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     4981 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     5041 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     5101 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct
     5161 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5221 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5281 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5341 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5401 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5461 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5521 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5641 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5701 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5761 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5821 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5881 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5941 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6001 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6481 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6541 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6601 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6661 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6721 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6781 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6841 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6901 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6961 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7021 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7081 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7141 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7201 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7261 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7321 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7381 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7441 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7501 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7561 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7621 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7681 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7741 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7981 ggggttcgcg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8341 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8401 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8521 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8581 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8641 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaaggga
//
