LOCUS       sequence-379886-        9458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for IGFBP2 with a fluorescent Clover 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Conway JRW, Dinc DD, Follain G, Paavolainen O, Kaivola J, Bostrom P,
            Hartiala P, Peuhu E, Ivaska J
  TITLE     IGFBP2 secretion by mammary adipocytes limits breast cancer 
            invasion.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg1840. doi: 10.1126/sciadv.adg1840. 
            Epub 2023 Jul 12.
  PUBMED    37436978
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg1840. doi"; pages: " 
            10.1126/sciadv.adg1840. Epub 2023 Jul 12"
COMMENT     Sequence Label: pLenti6.3/TO/V5-DEST-IGFBP2-Clover
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..30
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12..30
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        58..284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             285..465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    512..637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1130..1363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1548..1592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1859..1976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2302..2505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2456..2476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2507..2525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2528..2546
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2587..2611
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3642..3644,3645..3647,3648..4358)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3687..3708)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4295..4316
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4374..4398)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4451..4492
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4516..5104
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4569..4589)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4987..4998)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5119..5435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5286..5421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5483..5530
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5549..5947
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6034..6267
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6339..6460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6376..6395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6430..6449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6500..6635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6655..6674)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6656..6674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6684..6701)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6684..6700)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6693..6715)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6842..7297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6929..6948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7139..7160
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7323..7427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7428..7496,7497..8288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7646..7665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8459..9047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8948..8967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9201..9218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9335..9356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9371..9376,9377..9394,9395..9401)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9409..9425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9414..9436
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9433..9449
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9433..9449
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
       61 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
      121 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
      181 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
      241 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
      301 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      361 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      421 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      481 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      541 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      601 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      661 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      721 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      781 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      841 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      901 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      961 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1021 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1081 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1141 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1201 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1261 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1321 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1381 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1441 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1501 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1561 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1621 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1681 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1741 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1801 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     1861 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1921 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1981 ataagcttgg gagttccgct tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2041 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2101 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2161 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2221 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2281 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2341 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2401 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2461 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
     2521 agagatctcc ctatcagtga tagagatcgt cgactagtcc agtgtggtgg aattctgcag
     2581 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accaattcag tcgactggat
     2641 ccgccaccat gctgccgaga gtgggctgcc ccgcgctgcc gctgccgccg ccgccgctgc
     2701 tgccgctgct gccgctgctg ctgctgctac tgggcgcgag tggcggcggc ggcggggcgc
     2761 gcgcggaggt gctgttccgc tgcccgccct gcacacccga gcgcctggcc gcctgcgggc
     2821 ccccgccggt tgcgccgccc gccgcggtgg ccgcagtggc cggaggcgcc cgcatgccat
     2881 gcgcggagct cgtccgggag ccgggctgcg gctgctgctc ggtgtgcgcc cggctggagg
     2941 gcgaggcgtg cggcgtctac accccgcgct gcggccaggg gctgcgctgc tatccccacc
     3001 cgggctccga gctgcccctg caggcgctgg tcatgggcga gggcacttgt gagaagcgcc
     3061 gggacgccga gtatggcgcc agcccggagc aggttgcaga caatggcgat gaccactcag
     3121 aaggaggcct ggtggagaac cacgtggaca gcaccatgaa catgttgggc gggggaggca
     3181 gtgctggccg gaagcccctc aagtcgggta tgaaggagct ggccgtgttc cgggagaagg
     3241 tcactgagca gcaccggcag atgggcaagg gtggcaagca tcaccttggc ctggaggagc
     3301 ccaagaagct gcgaccaccc cctgccagga ctccctgcca acaggaactg gaccaggtcc
     3361 tggagcggat ctccaccatg cgccttccgg atgagcgggg ccctctggag cacctctact
     3421 ccctgcacat ccccaactgt gacaagcatg gcctgtacaa cctcaaacag tgcaagatgt
     3481 ctctgaacgg gcagcgtggg gagtgctggt gtgtgaaccc caacaccggg aagctgatcc
     3541 agggagcccc caccatccgg ggggaccccg agtgtcatct cttctacaat gagcagcagg
     3601 aggctcgcgg ggtgcacacc cagcggatgc agtgcggccg catggtgagc aagggcgagg
     3661 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3721 agttcagcgt ccgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt
     3781 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     3841 acggcgtggc ctgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3901 ccgccatgcc cgaaggctac gtccaggagc gcaccatctc tttcaaggac gacggtacct
     3961 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4021 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca
     4081 acagccacaa cgtctatatc acggccgaca agcagaagaa cggcatcaag gctaacttca
     4141 agatccgcca caacgttgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4201 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc catcagtccg
     4261 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4321 ccgccgggat tacacatggc atggacgagc tgtacaagta gtaagatatc tagacccagc
     4381 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
     4441 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     4501 ttagtaatga tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4561 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4621 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4681 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4741 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4801 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4861 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4921 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4981 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5041 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5101 ccgcctggcg atggtaccgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5161 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5281 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5341 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5401 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5461 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     5521 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac
     5581 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     5641 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     5701 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     5761 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     5821 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     5881 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     5941 gggctaagca caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6001 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6061 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6121 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6181 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6241 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6301 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6361 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6421 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6481 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6541 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6601 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6661 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6721 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6781 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6841 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6961 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7021 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7081 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7141 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7201 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7261 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7381 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7441 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7501 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7561 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7621 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7681 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7741 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7801 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7861 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7921 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7981 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8041 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8101 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8161 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8221 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8281 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8341 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8401 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8581 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8761 cgcagcggtc gggctgaacg gggggttcgc gcacacagcc cagcttggag cgaacgacct
     8821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9061 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9301 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9361 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9421 aacaatttca cacaggaaac agctatgacc atgattac
//
