LOCUS       sequence-379888-        8476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Conway JRW, Dinc DD, Follain G, Paavolainen O, Kaivola J, Bostrom P,
            Hartiala P, Peuhu E, Ivaska J
  TITLE     IGFBP2 secretion by mammary adipocytes limits breast cancer 
            invasion.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg1840. doi: 10.1126/sciadv.adg1840. 
            Epub 2023 Jul 12.
  PUBMED    37436978
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg1840. doi"; pages: " 
            10.1126/sciadv.adg1840. Epub 2023 Jul 12"
COMMENT     Sequence Label: pLenti6.3/TO/V5-DEST-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..265
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             266..446
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    493..618
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1111..1344
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1529..1573
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2283..2486
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2437..2457
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2488..2506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2509..2527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2630..2639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2636..2638,2639..2641,2642..3355)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2681..2702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2942..2961)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3289..3310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3373..3397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3450..3491
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3515..4103
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3568..3588)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3986..3997)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4118..4434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4285..4420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4482..4529
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4548..4946
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5033..5266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5338..5459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5375..5394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5429..5448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5499..5634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5654..5673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5655..5673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5683..5700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5683..5699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5692..5714)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5841..6296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5928..5947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6138..6159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6322..6426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6427..6495,6496..7287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6645..6664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7458..8046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7947..7966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8200..8217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8334..8355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8370..8375,8376..8393,8394..8400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8408..8424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8413..8435
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8432..8448
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8432..8448
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(8467..8476,1..11)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(8469..8476,1..11)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
       61 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
      121 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
      181 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
      241 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
      301 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      361 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      421 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      481 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      541 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      601 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
      661 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
      721 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
      781 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
      841 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
      901 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
      961 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1021 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1081 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1141 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1201 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1261 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1321 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1381 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1441 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1501 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1561 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1621 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1681 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     1741 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     1801 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttatc gataagcttg ggagttccgc
     1981 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2041 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2101 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2161 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2221 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2281 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2341 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2401 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2461 ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg
     2521 atagagatcg tcgactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg ccaccatggt
     2641 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2701 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2761 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2821 gaccaccctg tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     2881 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2941 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3001 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3061 ggagtacaac tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     3121 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     3181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3241 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaatgact
     3361 cgagatatct agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
     3421 gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
     3481 tcgattctac gcgtaccggt tagtaatgat cgacaatcaa cctctggatt acaaaatttg
     3541 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3601 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3661 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3721 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3781 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3841 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3901 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     3961 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4021 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4081 ctccctttgg gccgcctccc cgcctggcga tggtaccggt gtggaaagtc cccaggctcc
     4141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4201 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4261 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4441 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     4501 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt
     4561 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     4621 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     4681 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     4741 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     4801 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     4861 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     4921 cctctggtta tgtgtgggag ggctaagcac aattcgagct cggtaccttt aagaccaatg
     4981 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5041 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5101 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5161 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5221 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     5281 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     5341 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5401 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5461 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5521 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5581 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     5641 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     5701 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5761 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5821 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6181 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     6301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6661 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgcg cacacagccc
     7801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8101 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8161 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8221 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8281 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     8341 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     8401 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     8461 aagcgcgcaa ttaacc
//
