LOCUS       sequence-379895-        7082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To drive GCaMP6s under the control of mouse oxytocin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Yaguchi K, Hagihara M, Konno A, Hirai H, Yukinaga H, Miyamichi K
  TITLE     Dynamic modulation of pulsatile activities of oxytocin neurons in 
            lactating wild-type mice.
  JOURNAL   PLoS One. 2023 May 10;18(5):e0285589. doi: 
            10.1371/journal.pone.0285589. eCollection 2023.
  PUBMED    37163565
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-05-10"; volume: "18(5):e0285589. doi"; pages: " 
            10.1371/journal.pone.0285589. eCollection 2023"
COMMENT     Sequence Label: pAAV-OTp-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   34..163
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2772..2781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2778..2786,2787..2804,2805..2807,2808..2840,2841..2843,
                     2844..2867,2868..2894,2895..2951,2952..2957,2958..3227,
                     3228..3245,3246..3680,3681..3686,3687..4130)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      2874..2883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3164..3185
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3291..3312)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3552..3571)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4157..4278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4168..4187)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4222..4241
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4378..4518
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4378..4507
                     /label=AAV2 ITR
     rep_origin      4593..5048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4680..4699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4890..4911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5065..5084)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5184..5206
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5244..5262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5330..5434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5435..5503,5504..6295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5653..5672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6466..7054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6955..6974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
       61 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtaga
      181 tgagctggtg agcatgtgaa gacatgctgg cctaacaggc gcgttctgca catattttat
      241 gacatgctaa tttatgtgca tggattacct taggtgagat cactatgccc tcagaacatg
      301 caggctgtct ctctggggcc actttggggg tcaaactctt ggccttctat gcccagaatg
      361 ccaataggca attgtgggtg agaaatgaac tgatttgtag tacggttcca atcaacctca
      421 ccacccactt agcgttgata actcacacca gtgactcagg agagctgggg aacagggctt
      481 gccctgctgg ccccagcccc ttgaggagaa ggttgagcat aaggacagag cctaggggct
      541 tttcaattct ctccaggggc agagactgca tgtggtgagc tggtctctgg gacactggct
      601 gttacttctt cctggatgtt gcaggacact ttctaagaga gcctgagatg aagtttagaa
      661 catgggactg ggacccaccc ctgtggaaga gaaggggtac gggagcagag ccagtatgca
      721 gatctcagct aacctaaagc ccaaagctga agtgcagtca ggggtaccca gcctttgtac
      781 caaaatggac caactgctat atgtagatag ctgggaaggc cagctagctt tctgcagcta
      841 agaccacact ccggcagctg aggacaagtg ttttcttaaa caggagccta ggtacctggc
      901 atggtggtac acccagcacg tggcaggcag gtggatcact gtgagttcaa gcccagcctg
      961 gtctacacag acagctccag gccaaccaag agcctatctc aaaaaaaaaa aaaaaaaaaa
     1021 aacaaacgga agtctagaca atgcttctct tttccttttt aaaattgttt tagatctatt
     1081 tattttattt tacgtgtctg aatgatctgc ctgcatgtga gtaatgcact attcgtgtgc
     1141 cggctgctag gtcagaataa gtccctggct ccactggaat tgttgtaagt ggttgtgagc
     1201 caccctgtgg gtgctgggaa tcaaacctgg gtcttctgca agagtaactc tcagccccaa
     1261 gatagcaaga tatttaacat gaggcccagt ggatgatctt caggtcattg agggcaagcc
     1321 cttgaagtga gtagtgagac cttcatctcc tctggagagg tttttgattt ctcagccata
     1381 aaatgaaggg gttgagtctt ccgtgtcttc ctgctatgct ttgtctgtct gtctttcttt
     1441 tgttttggtt gattttggtt ttagtttgaa gccctggctg tcctggaact aacagaaatt
     1501 ctcctgcctc tgtctcctga gttctgggat taaaggtgtg cacccccagc accctctctt
     1561 gatatgtatt tcattttaag gggctggaga gatgattagc ctgcacactg ctctttcaga
     1621 gaaccaaatt ttgtcctggg cacccacata ggcctaccca tgatcccctt taatgacagc
     1681 tcctggggat ccagcaccct cttctggtcg ccaaggaaac ctgcgtgcac aaatgtacac
     1741 acacaaaatt aaaattgaaa tgcaaaaact gttcccaaaa tgtactgcta ccatcatgcg
     1801 ggggacttgc cccacccaac gtcgctcaca cactaggcaa gtactctgct actgggctgc
     1861 atgtaacacc cttcccatgc agacctatgc agacctgcag cccaaacctg aaatgtaccc
     1921 agagctgccc aattgattgc accaaaatgg gccaaccatt atatgtggcc acctgagaag
     1981 ggtatgacct tggcacaaat ggcttgctgt agctgaggcc acctgttggc cacactccag
     2041 cagctgatgg ccactgtttt ctcaaacagg agtctaggca cctagtgtgg tagtggataa
     2101 ctaaactcag catttgggag acagaagcag atggagcgct gtgagttcaa ggccagcctg
     2161 gtctacacag caggttctaa tacagagttt taaatactag tgtaattttc cttttgctgt
     2221 aatttttctt tctttttatt ttaatttggt ctgttaactg tgttttggtt ttgatctcta
     2281 ctgagacttt gttttacggg ctagttagaa gagctgaggt gcattcagag attgaacaag
     2341 acgccatctt tttccccatc taagtttcag gtccatgtaa gggctccctc actcacttgc
     2401 cctatcctgc cttattctga gatattggat atctgtgaaa aacagctcct ggctagggcg
     2461 cacctccaac ccctcccaag tctctctagc ctcttgtagc ctaggccacc ccttccaggc
     2521 tgcttctctt ttgagttcca ggtcattagc agagacgatg accttgaccc tagcccagac
     2581 cctgcaaatg aagggcctgc ctctaaacag cgtggaacaa tttgacccaa gagaccttct
     2641 gtgaccagtc atgctgtcac cctctttaga cagtgctcca ccatggcagt gcccagacat
     2701 aaaaaggtcg gtctgggccg gagaaaccat cacctacagc ggatctcaga ctgagcacca
     2761 tcgccgaatt cgccaccatg ggttctcatc atcatcatca tcatggtatg gctagcatga
     2821 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ctcgccacca
     2881 tggtcgactc atcacgtcgt aagtggaata agacaggtca cgcagtcaga gctataggtc
     2941 ggctgagctc actcgagaac gtctatatca aggccgacaa gcagaagaac ggcatcaagg
     3001 cgaacttcca catccgccac aacatcgagg acggcggcgt gcagctcgcc taccactacc
     3061 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcg
     3121 tgcagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggag
     3241 ggagcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3301 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca
     3361 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3421 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3481 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag gagcgcacca
     3541 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3601 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3661 ggcacaagct ggagtacaac ctgccggacc aactgactga agagcagatc gcagaattta
     3721 aagaggcttt ctccctattt gacaaggacg gggatgggac aataacaacc aaggagctgg
     3781 ggacggtgat gcggtctctg gggcagaacc ccacagaagc agagctgcag gacatgatca
     3841 atgaagtaga tgccgacggt gacggcacaa tcgacttccc tgagttcctg acaatgatgg
     3901 caagaaaaat gaaatacagg gacacggaag aagaaattag agaagcgttc ggtgtgtttg
     3961 ataaggatgg caatggctac atcagtgcag cagagcttcg ccacgtgatg acaaaccttg
     4021 gagagaagtt aacagatgaa gaggttgatg aaatgatcag ggaagcagac atcgatgggg
     4081 atggtcaggt aaactacgaa gagtttgtac aaatgatgac agcgaagtga gtcgacgccg
     4141 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4201 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4261 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4321 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtag cggccgcagg
     4381 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4441 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4501 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4561 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4621 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4681 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4801 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4921 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     5041 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5101 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5161 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5221 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5281 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5341 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5401 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6121 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6301 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7081 gt
//
