LOCUS       sequence-379909-       14537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for 
            generation of integration-free iPSCs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14537)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 14537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17
FEATURES             Location/Qualifiers
     source          1..14537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(79..99)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(497..508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(705..724)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    770..825
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(824..843)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1186..1202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1186..1202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1199..1221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1210..1226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1234..1240,1241..1258,1259..1264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1279..1300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1389..1422
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1883..3808
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGA
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGGGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    3976..4009
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4196..5985
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6526..6543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6697..7285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6777..6796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7456..8247,8248..8316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8079..8098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8317..8421)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8452..8831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8833..9110
                     /label=chicken beta-actin promoter
     intron          9111..10127
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10051..10073
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10135..10154
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             11269..11325
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    12754..13321
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    12771..13323
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12915..12932)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13142..13161
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 aagcttcggg gactagtcat atgataatca acctctggat tacaaaattt gtgaaagatt
       61 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
      241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
      301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
      361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
      421 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
      481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
      541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
      601 ggccgcctcc ccgcatcggt acaattcact cctcaggtgc aggctgccta tcagaaggtg
      661 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
      721 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      781 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      841 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
      901 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
      961 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     1021 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     1081 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     1141 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     1201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1321 cgctttccag tcgggaaacc tgtcgtgcca gcggatctca attccgatca tattcaataa
     1381 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg aagaggagtt
     1441 tacgtccagc caagcttagg atcaattctc atgtttgaca gcttatcatc gataagctga
     1501 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac
     1561 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg
     1621 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg
     1681 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa
     1741 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt
     1801 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta
     1861 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg
     1921 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag
     1981 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag
     2041 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc
     2101 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc
     2161 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg
     2221 aggggcagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
     2281 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg
     2341 ggcaggaggg gcaggagcag gaggaggggc aggaggcgca ggaggggcag gagcaggagg
     2401 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
     2461 aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagggg caggaggggc
     2521 aggagcagga ggggcaggag caggggcagg agcaggaggg gcaggagggg caggagcagg
     2581 aggggcagga gcaggagggg caggagcagg aggggcagga gcaggagggg caggaggggc
     2641 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     2701 aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gagggggagg
     2761 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
     2821 aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg
     2881 cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg
     2941 ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg
     3001 tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc
     3061 tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga
     3121 ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc
     3181 gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca
     3241 gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg
     3301 ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca
     3361 cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag
     3421 taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct
     3481 tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc
     3541 gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga
     3601 ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat
     3661 cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat
     3721 ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg
     3781 agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat
     3841 taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg
     3901 ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt
     3961 gccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
     4021 aagaggttca ctagcggatc tcaattgggc atacccatgt tgtcacgtca ctcagctccg
     4081 cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
     4141 atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga
     4201 aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag
     4261 cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg
     4321 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
     4381 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
     4441 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     4501 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcatatg
     4561 ctatccagat atttgggtag tatatgctac ccagatataa attaggatag catatactac
     4621 cctaatctct attaggatag catatgctac ccggatacag attaggatag catatactac
     4681 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     4741 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     4801 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat
     4861 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga
     4921 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat
     4981 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg
     5041 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg
     5101 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg
     5161 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct
     5221 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac
     5281 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa
     5341 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat
     5401 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc
     5461 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt
     5521 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa
     5581 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt
     5641 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg
     5701 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc
     5761 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa
     5821 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta
     5881 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag
     5941 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg
     6001 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag
     6061 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca
     6121 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg
     6181 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg
     6241 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc
     6301 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat
     6361 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg
     6421 ggcggggatc gatcctctag agtcgacgct agcggatccg ctgcattaat gaatcggcca
     6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     8461 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     8521 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     8581 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     8641 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8701 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     8761 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8821 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     8881 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     8941 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
     9001 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     9061 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     9121 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
     9181 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     9241 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     9301 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     9361 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     9421 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     9481 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     9541 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     9601 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     9661 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     9721 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     9781 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     9841 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     9901 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     9961 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
    10021 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
    10081 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
    10141 tgctggttat tgtgctgtct catcattttg gcaaagaatt cgcggccgcg tttaaacatg
    10201 gctggacacc tggcttcaga cttcgccttc tcacccccac caggtggggg tgatgggtca
    10261 gcagggctgg agccgggctg ggtggattct cgaacctggc taagcttcca agggcctcca
    10321 ggtgggcctg gaatcggacc aggctcagag gtattgggga tctccccatg tccgcccgca
    10381 tacgagttct gcggagggat ggcatactgt ggacctcagg ttggactggg cctagtcccc
    10441 caagttggcg tggagacttt gcagcctgag ggccaggcag gagcacgagt ggaaagcaac
    10501 tcagagggaa cctcctctga gccctgtgcc gaccgcccca atgccgtgaa gttggagaag
    10561 gtggaaccaa ctcccgagga gtcccaggac atgaaagccc tgcagaagga gctagaacag
    10621 tttgccaagc tgctgaagca gaagaggatc accttggggt acacccaggc cgacgtgggg
    10681 ctcaccctgg gcgttctctt tggaaaggtg ttcagccaga ccaccatctg tcgcttcgag
    10741 gccttgcagc tcagccttaa gaacatgtgt aagctgcggc ccctgctgga gaagtgggtg
    10801 gaggaagccg acaacaatga gaaccttcag gagatatgca aatcggagac cctggtgcag
    10861 gcccggaaga gaaagcgaac tagcattgag aaccgtgtga ggtggagtct ggagaccatg
    10921 tttctgaagt gcccgaagcc ctccctacag cagatcactc acatcgccaa tcagcttggg
    10981 ctagagaagg atgtggttcg agtatggttc tgtaaccggc gccagaaggg caaaagatca
    11041 agtattgagt attcccaacg agaagagtat gaggctacag ggacaccttt cccagggggg
    11101 gctgtatcct ttcctctgcc cccaggtccc cactttggca ccccaggcta tggaagcccc
    11161 cacttcacca cactctactc agtccctttt cctgagggcg aggcctttcc ctctgttccc
    11221 gtcactgctc tgggctctcc catgcattca aacacgcgtg gatctggagc caccaacttt
    11281 agtctcctca aacaagccgg tgacgttgaa gaaaatccag gccccatggc tgtcagcgac
    11341 gctctgctcc cgtccttctc cacgttcgcg tccggcccgg cgggaaggga gaagacactg
    11401 cgtccagcag gtgccccgac taaccgttgg cgtgaggaac tctctcacat gaagcgactt
    11461 cccccacttc ccggccgccc ctacgacctg gcggcgacgg tggccacaga cctggagagt
    11521 ggcggagctg gtgcagcttg cagcagtaac aacccggccc tcctagcccg gagggagacc
    11581 gaggagttca acgacctcct ggacctagac tttatccttt ccaactcgct aacccaccag
    11641 gaatcggtgg ccgccaccgt gaccacctcg gcgtcagctt catcctcgtc ttccccagcg
    11701 agcagcggcc ctgccagcgc gccctccacc tgcagcttca gctatccgat ccgggccggg
    11761 ggtgacccgg gcgtggctgc cagcaacaca ggtggagggc tcctctacag ccgagaatct
    11821 gcgccacctc ccacggcccc cttcaacctg gcggacatca atgacgtgag cccctcgggc
    11881 ggcttcgtgg ctgagctcct gcggccggag ttggacccag tatacattcc gccacagcag
    11941 cctcagccgc caggtggcgg gctgatgggc aagtttgtgc tgaaggcgtc tctgaccacc
    12001 cctggcagcg agtacagcag cccttcggtc atcagtgtta gcaaaggaag cccagacggc
    12061 agccaccccg tggtagtggc gccctacagc ggtggcccgc cgcgcatgtg ccccaagatt
    12121 aagcaagagg cggtcccgtc ctgcacggtc agccggtccc tagaggccca tttgagcgct
    12181 ggaccccagc tcagcaacgg ccaccggccc aacacacacg acttccccct ggggcggcag
    12241 ctccccacca ggactacccc tacactgagt cccgaggaac tgctgaacag cagggactgt
    12301 caccctggcc tgcctcttcc cccaggattc catccccatc cggggcccaa ctaccctcct
    12361 ttcctgccag accagatgca gtcacaagtc ccctctctcc attatcaaga gctcatgcca
    12421 ccgggttcct gcctgccaga ggagcccaag ccaaagaggg gaagaaggtc gtggccccgg
    12481 aaaagaacag ccacccacac ttgtgactat gcaggctgtg gcaaaaccta taccaagagt
    12541 tctcatctca aggcacacct gcgaactcac acaggcgaga aaccttacca ctgtgactgg
    12601 gacggctgtg ggtggaaatt cgcccgctcg gatgaactga ccaggcacta ccgcaaacac
    12661 acagggcacc ggccctttca gtgccagaag tgcgacaggg ccttttccag gtcggaccac
    12721 cttgccttac acatgaagag gcacttttaa agatccctcc ccccccccta acgttactgg
    12781 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
    12841 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
    12901 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
    12961 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
    13021 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
    13081 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
    13141 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
    13201 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
    13261 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
    13321 atatggccac acatatgatg tataacatga tggagacgga gctgaagccg ccgggcccgc
    13381 agcaagcttc ggggggcggc ggcggaggag gcaacgccac ggcggcggca accggcggca
    13441 accagaagaa cagcccggac cgcgtcaaga ggcccatgaa cgccttcatg gtatggtccc
    13501 gggggcagcg gcgtaagatg gcccaggaga accccaagat gcacaactcg gagatcagca
    13561 agcgcctggg cgcggagtgg aaagcgttga ccttggcaga gaagcggccg ttcatcgacg
    13621 aggccaagcg gctgcgcgtg ctgcatatgc aggaccaccc caactacaag taccggccgc
    13681 ggcggcgcaa gcaggtgaag cgcatgaagc gggtggaggg aggcttcctg cacgctctcg
    13741 tcgagcccca ggccggcgcg cttggtcccg agggcggccg cgtggccatg gatggcctgg
    13801 gtctgccttt cccggagccg ggctatccgg ccggtcctcc gctgatgtct ccgcacatgg
    13861 gcccccacta tcgggactgc cagggactgg gcgctcccgc gctcgacggc taccctctgc
    13921 ccactccgga cacatccccg ctggatggcg tggagcagga cccggctttc tttgcagccc
    13981 cgctgccagg ggactgcccg gcggccggca cctacactta cgctccagtc tcggactatg
    14041 cagtgtccgt agagccgccc gctggcccca tgcgagtggg gccggacccc tcgggccctg
    14101 cgatgccggg gatcctggcg ccccccagcg ctctgcacct gtactacggc gcgatgggct
    14161 cgcccgccgc aagtgcgggg cgcggtttcc acgcgcaacc ccagcagccg ctgcaaccgc
    14221 aggcaccgcc cccgccaccg cagcagcagc acccagcgca cggccccggg caaccttcgc
    14281 cccctcccga ggctctgccc tgccgggatg gcacggaatc caaccagccc actgagctcc
    14341 taggggaggt ggaccgcacg gaatttgaac agtatctgcc ctttgtgtat aagcccgaga
    14401 tgggtcttcc ctaccaggga cacgactgcg gagtgaacct ctcagacagc cacggagcca
    14461 tttcctccgt ggtgtccgac gctagctcag cggtctacta ttgcaactac cccgacattt
    14521 gataaatcga agaattc
//
