LOCUS       sequence-379922-       16106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monitor HIV-1 fate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16106)
  AUTHORS   Ratnapriya S, Harris M, Chov A, Herbert ZT, Vrbanac V, Deruaz M, 
            Achuthan V, Engelman AN, Sodroski J, Herschhorn A
  TITLE     Intra- and extra-cellular environments contribute to the fate of 
            HIV-1 infection.
  JOURNAL   Cell Rep. 2021 Aug 31;36(9):109622. doi: 
            10.1016/j.celrep.2021.109622.
  PUBMED    34469717
REFERENCE   2  (bases 1 to 16106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-08-31"; volume: "36(9):109622. doi"; pages: " 
            10.1016/j.celrep.2021.109622"
COMMENT     Sequence Label: HI.fate.SFFV
FEATURES             Location/Qualifiers
     source          1..16106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             461..718
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             874..2376
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2169..5180
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4865..4982
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6620..6853
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7038..7082
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7376..7402
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7666..8340
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHMA
                     LKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     8061..8080
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8358..8765
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8767..8776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8773..9465
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     CDS             9475..9531
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(9532..9534,9535..9537,9538..10251)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9532..9534,9535..9537,9538..10248)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9577..9598)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9838..9857)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10185..10206
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             10441..11074
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12714..12731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12885..13473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12965..12984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13644..14435,14436..14504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14267..14286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14505..14609)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14677..14695
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14733..14755)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14855..14874
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggagggacc agcgcagctt ccagccatcc acctgatgaa cagaacctag ggaaagcccc
       61 agttctactt acaccaggaa aggctggaag ggctaatttg gtcccaaaaa agacaagaga
      121 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
      181 cagggccagg gatcagatat ccactgacct ttggatggtg cttcaagtta gtaccagttg
      241 aaccagagca agtagaagag gccaatgaag gagagaacaa cagcttgtta caccctatga
      301 gccagcatgg gatggaggac ccggagggag aagtattagt gtggaagttt gacagcctcc
      361 tagcatttcg tcacatggcc cgagagctgc atccggagta ctacaaagac tgctgacatc
      421 gagctttcta caagggactt tccgctgggg actttccagg gaggtgtggc ctgggcggga
      481 ctggggagtg gcgagccctc agatgctaca tataagcagc tgctttttgc ctgtactggg
      541 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      601 cttaagcctc aataaagctt gccttgagtg ctcaaagtag tgtgtgcccg tctgttgtgt
      661 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      721 ggcgcccgaa cagggacttg aaagcgaaag taaagccaga ggagatctct cgacgcagga
      781 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      841 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc ggtattaagc
      901 gggggagaat tagataaatg ggaaaaaatt cggttaaggc cagggggaaa gaaacaatat
      961 aaactaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1021 cttttagaga catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1081 acaggatcag aagaacttag atcattatat aatacaatag cagtcctcta ttgtgtgcat
     1141 caaaggatag atgtaaaaga caccaaggaa gccttagata agatagagga agagcaaaac
     1201 aaaagtaaga aaaaggcaca gcaagcagca gctgacacag gaaacaacag ccaggtcagc
     1261 caaaattacc ctatagtgca gaacctccag gggcaaatgg tacatcaggc catatcacct
     1321 agaactttaa atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtaata
     1381 cccatgtttt cagcattatc agaaggagcc accccacaag atttaaatac catgctaaac
     1441 acagtggggg gacatcaagc agccatgcaa atgttaaaag agaccatcaa tgaggaagct
     1501 gcagaatggg atagattgca tccagtgcat gcagggccta ttgcaccagg ccagatgaga
     1561 gaaccaaggg gaagtgacat agcaggaact actagtaccc ttcaggaaca aataggatgg
     1621 atgacacata atccacctat cccagtagga gaaatctata aaagatggat aatcctggga
     1681 ttaaataaaa tagtaagaat gtatagccct accagcattc tggacataag acaaggacca
     1741 aaggaaccct ttagagacta tgtagaccga ttctataaaa ctctaagagc cgagcaagct
     1801 tcacaagagg taaaaaattg gatgacagaa accttgttgg tccaaaatgc gaacccagat
     1861 tgtaagacta ttttaaaagc attgggacca ggagcgacac tagaagaaat gatgacagca
     1921 tgtcagggag tggggggacc cggccataaa gcaagagttt tggctgaagc aatgagccaa
     1981 gtaacaaatc cagctaccat aatgatacag aaaggcaatt ttaggaacca aagaaagact
     2041 gttaagtgtt tcaattgtgg caaagaaggg cacatagcca aaaattgcag ggcccctagg
     2101 aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg tactgagaga
     2161 caggctaatt ttttagggaa gatctggcct tcccacaagg gaaggccagg gaattttctt
     2221 cagagcagac cagagccaac agccccacca gaagagagct tcaggtttgg ggaagagaca
     2281 acaactccct ctcagaagca ggagccgata gacaaggaac tgtatccttt agcttccctc
     2341 agatcactct ttggcagcga cccctcgtca caataaagat aggggggcaa ttaaaggaag
     2401 ctctattaga tacaggagca gatgatacag tattagaaga aatgaatttg ccaggaagat
     2461 ggaaaccaaa aatgataggg ggaattggag gttttatcaa agtaagacag tatgatcaga
     2521 tactcataga aatctgcgga cataaagcta taggtacagt attagtagga cctacacctg
     2581 tcaacataat tggaagaaat ctgttgactc agattggctg cactttaaat tttcccatta
     2641 gtcctattga gactgtacca gtaaaattaa agccaggaat ggatggccca aaagttaaac
     2701 aatggccatt gacagaagaa aaaataaaag cattagtaga aatttgtaca gaaatggaaa
     2761 aggaaggaaa aatttcaaaa attgggcctg aaaatccata caatactcca gtatttgcca
     2821 taaagaaaaa agacagtact aaatggagaa aattagtaga tttcagagaa cttaataaga
     2881 gaactcaaga tttctgggaa gttcaattag gaataccaca tcctgcaggg ttaaaacaga
     2941 aaaaatcagt aacagtactg gatgtgggcg atgcatattt ttcagttccc ttagataaag
     3001 acttcaggaa gtatactgca tttaccatac ctagtataaa caatgagaca ccagggatta
     3061 gatatcagta caatgtgctt ccacagggat ggaaaggatc accagcaata ttccagtgta
     3121 gcatgacaaa aatcttagag ccttttagaa aacaaaatcc agacatagtc atctatcaat
     3181 acatggatga tttgtatgta ggatctgact tagaaatagg gcagcataga acaaaaatag
     3241 aggaactgag acaacatctg ttgaggtggg gatttaccac accagacaaa aaacatcaga
     3301 aagaacctcc attcctttgg atgggttatg aactccatcc tgataaatgg acagtacagc
     3361 ctatagtgct gccagaaaag gacagctgga ctgtcaatga catacagaaa ttagtgggaa
     3421 aattgaattg ggcaagtcag atttatgcag ggattaaagt aaggcaatta tgtaaacttc
     3481 ttaggggaac caaagcacta acagaagtag taccactaac agaagaagca gagctagaac
     3541 tggcagaaaa cagggagatt ctaaaagaac cggtacatgg agtgtattat gacccatcaa
     3601 aagacttaat agcagaaata cagaagcagg ggcaaggcca atggacatat caaatttatc
     3661 aagagccatt taaaaatctg aaaacaggaa agtatgcaag aatgaagggt gcccacacta
     3721 atgatgtgaa acaattaaca gaggcagtac aaaaaatagc cacagaaagc atagtaatat
     3781 ggggaaagac tcctaaattt aaattaccca tacaaaagga aacatgggaa gcatggtgga
     3841 cagagtattg gcaagccacc tggattcctg agtgggagtt tgtcaatacc cctcccttag
     3901 tgaagttatg gtaccagtta gagaaagaac ccataatagg agcagaaact ttctatgtag
     3961 atggggcagc caatagggaa actaaattag gaaaagcagg atatgtaact gacagaggaa
     4021 gacaaaaagt tgtcccccta acggacacaa caaatcagaa gactgagtta caagcaattc
     4081 atctagcttt gcaggattcg ggattagaag taaacatagt gacagactca caatatgcat
     4141 tgggaatcat tcaagcacaa ccagataaga gtgaatcaga gttagtcagt caaataatag
     4201 agcagttaat aaaaaaggaa aaagtctacc tggcatgggt accagcacac aaaggaattg
     4261 gaggaaatga acaagtagat aaattggtca gtgctggaat caggaaagta ctatttttag
     4321 atggaataga taaggcccaa gaagaacatg agaaatatca cagtaattgg agagcaatgg
     4381 ctagtgattt taacctacca cctgtagtag caaaagaaat agtagccagc tgtgataaat
     4441 gtcagctaaa aggggaagcc atgcatggac aagtagactg tagcccagga atatggcagc
     4501 tagattgtac acatttagaa ggaaaagtta tcttggtagc agttcatgta gccagtggat
     4561 atatagaagc agaagtaatt ccagcagaga cagggcaaga aacagcatac ttcctcttaa
     4621 aattagcagg aagatggcca gtaaaaacag tacatacaga caatggcagc aatttcacca
     4681 gtactacagt taaggccgcc tgttggtggg cggggatcaa gcaggaattt ggcattccct
     4741 acaatcccca aagtcaagga gtaatagaat ctatgaataa agaattaaag aaaattatag
     4801 gacaggtaag agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc
     4861 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     4921 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     4981 ttcgggttta ttacagggac agcagagatc cagtttggaa aggaccagca aagctcctct
     5041 ggaaaggtga aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa
     5101 gaaaagcaaa gatcatcagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa
     5161 gtagacagga tgaggattaa cacatggaaa agattagtaa aacaccatat gtatatttca
     5221 aggaaagcta aggactggtt ttatagacat cactatgaaa gtactaatcc aaaaataagt
     5281 tcagaagtac acatcccact aggggatgct aaattagtaa taacaacata ttggggtctg
     5341 catacaggag aaagagactg gcatttgggt cagggagtct ccatagaatg gaggaaaaag
     5401 agatatagca cacaagtaga ccctgaccta gcagaccaac taattcatct gcactatttt
     5461 gattgttttt cagaatctgc tataagaaat accatattag gacgtatagt tagtcctagg
     5521 tgtgaatatc aagcaggaca taacaaggta ggatctctac agtacttggc actagcagca
     5581 ttaataaaac caaaacagat aaagccacct ttgcctagtg ttaggaaact gacagaggac
     5641 agatggaaca agccccagaa gaccaagggc cacagaggga gccatacaat gaatggacac
     5701 tagagctttt agaggaactt aattaagagt gaagctgtta gacattttcc taggatatgg
     5761 ctccataact taggacaaca tatctatgaa acttacgggg atacttgggc aggagtggaa
     5821 gccataataa gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat
     5881 agcagaatag gcgttactcg acagaggaga gcaagaaatg gagccagtag atcctagact
     5941 agagccctgg aagcatccag gaagtcagcc taaaactgct tgtaccaatt gctattgtaa
     6001 aaagtgttgc tttcattgcc aagtttgttt catgacaaaa gccttaggca tctcctatgg
     6061 caggaagaag cggagacagc gacgaagagc tcatcagaac agtcagactc atcaagcttc
     6121 tctatcaaag cagtaagtag tacatgtaat gcaacctata atagtagcaa tagtagcatt
     6181 agtagtagca ataataatag caatagttgt gtggtccata gtaatcatag aatataggaa
     6241 aatattaaga caaagaaaaa tagacaggtt aattgataga ctaatagaaa gagcagaaga
     6301 cagtggcaat gagagtgaag gagaagtatc agcacttgtg gagatggggg tggaaatggg
     6361 gcaccatgct ccttgggata ttgatgatct gtagtgctta gtaaggatgt tcatcaaata
     6421 ttactgggct gctattaaca agagatggtg gtaataacaa caatgggtcc gagatcttca
     6481 gacctggagg aggcgatatg agggacaatt ggagaagtga attatataaa tataaagtag
     6541 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     6601 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     6661 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctgatatag
     6721 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     6781 cagtctgggg catcaaacag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     6841 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     6901 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaataac atgacctgga
     6961 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7021 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7081 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7141 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7201 ggcagggata ttcaccatta tcgtttcaga cccacctccc aatcccgagg ggacccgaca
     7261 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     7321 tgaacggatc cttagcactt atctgggacg atctgcggag cctgtgcctc ttcagctacc
     7381 accgcttgag agacttactc ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg
     7441 ggtgggaagc cctcaaatat tggtggaatc tcctacaata ttggagtcag gagctaaaga
     7501 atagtgctgt tagcttgctc aatgccacag ccatagcagt agctgagggg acagataggg
     7561 ttatagaagt agtacaagga gcttgtagag ctattcgcca catacctaga agaataagac
     7621 agggcttgga aaggattttg ctataagatg ggtggcgcgg ccgctgatag cactgagaac
     7681 gtcatcaagc ccttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7741 ttcgagatcg agggcgtggg cgagggcaag ccctacgagg gcacccagac cgccaagctg
     7801 caagtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcttc
     7861 tacggctcca aggcgtacat caagcacccc gccgacatcc ccgactacct caagcagtcc
     7921 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7981 gtgacccagg actcctctct gcaggacggc accctcatct accacgtgaa gttcatcggc
     8041 gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ctctgggctg ggagccctcc
     8101 actgagcgca actacccccg cgacggcgtg ctgaagggcg agaaccacat ggcgctgaag
     8161 ctgaagggcg gcggccacta cctgtgtgag ttcaagtcca tctacatggc caagaagccc
     8221 gtgaagctgc ccggctacca ctacgtggac tacaagctcg acatcacctc ccacaacgag
     8281 gactacaccg tggtggagca gtacgagcgc gccgaggccc gccaccacct gttccagtag
     8341 cctgcagacg gccgcgggta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa
     8401 tagagaagtt cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat
     8461 atctgcggtg agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt
     8521 cggccccggc ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt
     8581 tcttaagacc catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt
     8641 atttgaatta accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc
     8701 tctataaaag agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc
     8761 gtacgcgcca ccatggccca gtccaagcac ggcctgacca aggagatgac catgaagtac
     8821 cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg catcggctac
     8881 cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc cttgcccttc
     8941 gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac cgagtacccc
     9001 caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg ggaccgctcc
     9061 ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt gagcgtggag
     9121 gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc cgacggcccc
     9181 gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat ccccgtgccc
     9241 aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga cggtggccgc
     9301 ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa gatgcccgac
     9361 tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa gaaccagaag
     9421 tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgcccggaag tggggcgacc
     9481 aacttctctt tgcttaaaca ggctggtgac gttgaagaga atcccgggcc gatggtgagc
     9541 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     9601 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     9661 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     9721 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     9781 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     9841 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     9901 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     9961 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
    10021 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
    10081 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
    10141 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
    10201 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta actcgagacc
    10261 tagaaaaaca tggagcaatc acaagtagca atacagcagc taacaatgct gcttgtgcct
    10321 ggctagaagc acaagaggag gaagaggtgg gttttccagt cacacctcag gtacctttaa
    10381 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    10441 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
    10501 cacaaggcta cttccctgat tggcagaact acacaccagg gccaggggtc agatatccac
    10561 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
    10621 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgaccctg
    10681 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
    10741 agctgcatcc ggagtacttc aagaactgct gacatcgagc ttgctacaag ggactttccg
    10801 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
    10861 gctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
    10921 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10981 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    11041 agaccctttt agtcagtgtg gaaaatctct agcacccagg aggtagaggt tgcagtgagc
    11101 caagatcgcg ccactgcatt ccagcctggg caagaaaaca agactgtcta aaataataat
    11161 aataagttaa gggtattaaa tatatttata catggaggtc ataaaaatat atatatttgg
    11221 gctgggcgca gtggctcaca cctgcgcccg gccctttggg aggccgaggc aggtggatca
    11281 cctgagtttg ggagttccag accagcctga ccaacatgga gaaacccctt ctctgtgtat
    11341 ttttagtaga ttttatttta tgtgtatttt attcacaggt atttctggaa aactgaaact
    11401 gtttttcctc tactctgata ccacaagaat catcagcaca gaggaagact tctgtgatca
    11461 aatgtggtgg gagagggagg ttttcaccag cacatgagca gtcagttctg ccgcagactc
    11521 ggcgggtgtc cttcggttca gttccaacac cgcctgcctg gagagaggtc agaccacagg
    11581 gtgagggctc agtccccaag acataaacac ccaagacata aacacccaac aggtccaccc
    11641 cgcctgctgc ccaggcagag ccgattcacc aagacgggaa ttaggataga gaaagagtaa
    11701 gtcacacaga gccggctgtg cgggagaacg gagttctatt atgactcaaa tcagtctccc
    11761 caagcattcg gggatcagag tttttaagga taacttagtg tgtagggggc cagtgagttg
    11821 gagatgaaag cgtagggagt cgaaggtgtc cttttgcgcc gagtcagttc ctgggtgggg
    11881 gccacaagat cggatgagcc agtttatcaa tccgggggtg ccagctgatc catggagtgc
    11941 agggtctgca aaatatctca agcactgatt gatcttaggt tttacaatag tgatgttacc
    12001 ccaggaacaa tttggggaag gtcagaatct tgtagcctgt agctgcatga ctcctaaacc
    12061 ataatttctt ttttgttttt ttttttttat ttttgagaca gggtctcact ctgtcaccta
    12121 ggctggagtg cagtggtgca atcacagctc actgcagcct caacgtcgta agctcaagcg
    12181 atcctcccac ctcagcctgc ctggtagctg agactacaag cgacgcccca gttaattttt
    12241 gtatttttgg tagaggcagc gttttgccgt gtggccctgg ctggtctcga actcctgggc
    12301 tcaagtgatc cagcctcagc ctcccaaagt gctgggacaa ccggggccag tcactgcacc
    12361 tggccctaaa ccataatttc taatcttttg gctaatttgt tagtcctaca aaggcagtct
    12421 agtccccagg caaaaagggg gtttgtttcg ggaaagggct gttactgtct ttgtttcaaa
    12481 ctataaacta agttcctcct aaacttagtt cggcctacac ccaggaatga acaaggagag
    12541 cttggaggtt agaagcacga tggaattggt taggtcagat ctctttcact gtctgagtta
    12601 taattttgca atggtggttc aaagactgcc cgcttctgac accagtcgct gcattaatga
    12661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    12721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    12841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    12901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    13021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    13081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    13141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    13201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    13261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    13321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    13381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    13441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    13501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    13561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    13621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    13681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    13741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    13801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    13861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    13921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    13981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    14041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    14101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    14161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    14221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    14281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    14341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    14401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    14461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    14521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    14581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    14641 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    14701 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    14761 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    14821 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    14881 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    14941 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    15001 ctattacgcc aggggaggca gagattgcag taagctgaga tcgcagcact gcactccagc
    15061 ctgggcgaca gagtaagact ctgtctcaaa aataaaataa ataaatcaat cagatattcc
    15121 aatcttttcc tttatttatt tatttatttt ctattttgga aacacagtcc ttccttattc
    15181 cagaattaca catatattct atttttcttt atatgctcca gtttttttta gaccttcacc
    15241 tgaaatgtgt gtatacaaaa tctaggccag tccagcagag cctaaaggta aaaaataaaa
    15301 taataaaaaa taaataaaat ctagctcact ccttcacatc aaaatggaga tacagctgtt
    15361 agcattaaat accaaataac ccatcttgtc ctcaataatt ttaagcgcct ctctccacca
    15421 catctaactc ctgtcaaagg catgtgcccc ttccgggcgc tctgctgtgc tgccaaccaa
    15481 ctggcatgtg gactctgcag ggtccctaac tgccaagccc cacagtgtgc cctgaggctg
    15541 ccccttcctt ctagcggctg cccccactcg gctttgcttt ccctagtttc agttacttgc
    15601 gttcagccaa ggtctgaaac taggtgcgca cagagcggta agactgcgag agaaagagac
    15661 cagctttaca gggggtttat cacagtgcac cctgacagtc gtcagcctca cagggggttt
    15721 atcacattgc accctgacag tcgtcagcct cacagggggt ttatcacagt gcacccttac
    15781 aatcattcca tttgattcac aattttttta gtctctactg tgcctaactt gtaagttaaa
    15841 tttgatcaga ggtgtgttcc cagaggggaa aacagtatat acagggttca gtactatcgc
    15901 atttcaggcc tccacctggg tcttggaatg tgtcccccga ggggtgatga ctacctcagt
    15961 tggatctcca caggtcacag tgacacaaga taaccaagac acctcccaag gctaccacaa
    16021 tgggccgccc tccacgtgca catggccgga ggaactgcca tgtcggaggt gcaagcacac
    16081 ctgcgcatca gagtccttgg tgtgga
//
