LOCUS       sequence-379931-       16261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monitor HIV-1 fate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16261)
  AUTHORS   Ratnapriya S, Harris M, Chov A, Herbert ZT, Vrbanac V, Deruaz M, 
            Achuthan V, Engelman AN, Sodroski J, Herschhorn A
  TITLE     Intra- and extra-cellular environments contribute to the fate of 
            HIV-1 infection.
  JOURNAL   Cell Rep. 2021 Aug 31;36(9):109622. doi: 
            10.1016/j.celrep.2021.109622.
  PUBMED    34469717
REFERENCE   2  (bases 1 to 16261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-08-31"; volume: "36(9):109622. doi"; pages: " 
            10.1016/j.celrep.2021.109622"
COMMENT     Sequence Label: HI.fate.E
FEATURES             Location/Qualifiers
     source          1..16261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             209..466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             622..2124
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1917..4928
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4613..4730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6368..6601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6786..6830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7124..7150
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7414..8088
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHMA
                     LKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     7809..7828
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8134..8345
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             8358..8626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      8670..8679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8676..9368
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     CDS             9378..9434
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(9435..9437,9438..9440,9441..10154)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9435..9437,9438..9440,9441..10151)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9480..9501)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9741..9760)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10088..10109
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             10344..10977
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12617..12634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12788..13376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12868..12887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13547..14338,14339..14407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14170..14189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14408..14512)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14580..14598
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14636..14658)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14758..14777
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagaagaggc caatgaagga gagaacaaca gcttgttaca ccctatgagc cagcatggga
       61 tggaggaccc ggagggagaa gtattagtgt ggaagtttga cagcctccta gcatttcgtc
      121 acatggcccg agagctgcat ccggagtact acaaagactg ctgacatcga gctttctaca
      181 agggactttc cgctggggac tttccaggga ggtgtggcct gggcgggact ggggagtggc
      241 gagccctcag atgctacata taagcagctg ctttttgcct gtactgggtc tctctggtta
      301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      361 taaagcttgc cttgagtgct caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      481 gggacttgaa agcgaaagta aagccagagg agatctctcg acgcaggact cggcttgctg
      541 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      601 gcggaggcta gaaggagaga gatgggtgcg agagcgtcgg tattaagcgg gggagaatta
      661 gataaatggg aaaaaattcg gttaaggcca gggggaaaga aacaatataa actaaaacat
      721 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct tttagagaca
      781 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
      841 gaacttagat cattatataa tacaatagca gtcctctatt gtgtgcatca aaggatagat
      901 gtaaaagaca ccaaggaagc cttagataag atagaggaag agcaaaacaa aagtaagaaa
      961 aaggcacagc aagcagcagc tgacacagga aacaacagcc aggtcagcca aaattaccct
     1021 atagtgcaga acctccaggg gcaaatggta catcaggcca tatcacctag aactttaaat
     1081 gcatgggtaa aagtagtaga agagaaggct ttcagcccag aagtaatacc catgttttca
     1141 gcattatcag aaggagccac cccacaagat ttaaatacca tgctaaacac agtgggggga
     1201 catcaagcag ccatgcaaat gttaaaagag accatcaatg aggaagctgc agaatgggat
     1261 agattgcatc cagtgcatgc agggcctatt gcaccaggcc agatgagaga accaagggga
     1321 agtgacatag caggaactac tagtaccctt caggaacaaa taggatggat gacacataat
     1381 ccacctatcc cagtaggaga aatctataaa agatggataa tcctgggatt aaataaaata
     1441 gtaagaatgt atagccctac cagcattctg gacataagac aaggaccaaa ggaacccttt
     1501 agagactatg tagaccgatt ctataaaact ctaagagccg agcaagcttc acaagaggta
     1561 aaaaattgga tgacagaaac cttgttggtc caaaatgcga acccagattg taagactatt
     1621 ttaaaagcat tgggaccagg agcgacacta gaagaaatga tgacagcatg tcagggagtg
     1681 gggggacccg gccataaagc aagagttttg gctgaagcaa tgagccaagt aacaaatcca
     1741 gctaccataa tgatacagaa aggcaatttt aggaaccaaa gaaagactgt taagtgtttc
     1801 aattgtggca aagaagggca catagccaaa aattgcaggg cccctaggaa aaagggctgt
     1861 tggaaatgtg gaaaggaagg acaccaaatg aaagattgta ctgagagaca ggctaatttt
     1921 ttagggaaga tctggccttc ccacaaggga aggccaggga attttcttca gagcagacca
     1981 gagccaacag ccccaccaga agagagcttc aggtttgggg aagagacaac aactccctct
     2041 cagaagcagg agccgataga caaggaactg tatcctttag cttccctcag atcactcttt
     2101 ggcagcgacc cctcgtcaca ataaagatag gggggcaatt aaaggaagct ctattagata
     2161 caggagcaga tgatacagta ttagaagaaa tgaatttgcc aggaagatgg aaaccaaaaa
     2221 tgataggggg aattggaggt tttatcaaag taagacagta tgatcagata ctcatagaaa
     2281 tctgcggaca taaagctata ggtacagtat tagtaggacc tacacctgtc aacataattg
     2341 gaagaaatct gttgactcag attggctgca ctttaaattt tcccattagt cctattgaga
     2401 ctgtaccagt aaaattaaag ccaggaatgg atggcccaaa agttaaacaa tggccattga
     2461 cagaagaaaa aataaaagca ttagtagaaa tttgtacaga aatggaaaag gaaggaaaaa
     2521 tttcaaaaat tgggcctgaa aatccataca atactccagt atttgccata aagaaaaaag
     2581 acagtactaa atggagaaaa ttagtagatt tcagagaact taataagaga actcaagatt
     2641 tctgggaagt tcaattagga ataccacatc ctgcagggtt aaaacagaaa aaatcagtaa
     2701 cagtactgga tgtgggcgat gcatattttt cagttccctt agataaagac ttcaggaagt
     2761 atactgcatt taccatacct agtataaaca atgagacacc agggattaga tatcagtaca
     2821 atgtgcttcc acagggatgg aaaggatcac cagcaatatt ccagtgtagc atgacaaaaa
     2881 tcttagagcc ttttagaaaa caaaatccag acatagtcat ctatcaatac atggatgatt
     2941 tgtatgtagg atctgactta gaaatagggc agcatagaac aaaaatagag gaactgagac
     3001 aacatctgtt gaggtgggga tttaccacac cagacaaaaa acatcagaaa gaacctccat
     3061 tcctttggat gggttatgaa ctccatcctg ataaatggac agtacagcct atagtgctgc
     3121 cagaaaagga cagctggact gtcaatgaca tacagaaatt agtgggaaaa ttgaattggg
     3181 caagtcagat ttatgcaggg attaaagtaa ggcaattatg taaacttctt aggggaacca
     3241 aagcactaac agaagtagta ccactaacag aagaagcaga gctagaactg gcagaaaaca
     3301 gggagattct aaaagaaccg gtacatggag tgtattatga cccatcaaaa gacttaatag
     3361 cagaaataca gaagcagggg caaggccaat ggacatatca aatttatcaa gagccattta
     3421 aaaatctgaa aacaggaaag tatgcaagaa tgaagggtgc ccacactaat gatgtgaaac
     3481 aattaacaga ggcagtacaa aaaatagcca cagaaagcat agtaatatgg ggaaagactc
     3541 ctaaatttaa attacccata caaaaggaaa catgggaagc atggtggaca gagtattggc
     3601 aagccacctg gattcctgag tgggagtttg tcaatacccc tcccttagtg aagttatggt
     3661 accagttaga gaaagaaccc ataataggag cagaaacttt ctatgtagat ggggcagcca
     3721 atagggaaac taaattagga aaagcaggat atgtaactga cagaggaaga caaaaagttg
     3781 tccccctaac ggacacaaca aatcagaaga ctgagttaca agcaattcat ctagctttgc
     3841 aggattcggg attagaagta aacatagtga cagactcaca atatgcattg ggaatcattc
     3901 aagcacaacc agataagagt gaatcagagt tagtcagtca aataatagag cagttaataa
     3961 aaaaggaaaa agtctacctg gcatgggtac cagcacacaa aggaattgga ggaaatgaac
     4021 aagtagataa attggtcagt gctggaatca ggaaagtact atttttagat ggaatagata
     4081 aggcccaaga agaacatgag aaatatcaca gtaattggag agcaatggct agtgatttta
     4141 acctaccacc tgtagtagca aaagaaatag tagccagctg tgataaatgt cagctaaaag
     4201 gggaagccat gcatggacaa gtagactgta gcccaggaat atggcagcta gattgtacac
     4261 atttagaagg aaaagttatc ttggtagcag ttcatgtagc cagtggatat atagaagcag
     4321 aagtaattcc agcagagaca gggcaagaaa cagcatactt cctcttaaaa ttagcaggaa
     4381 gatggccagt aaaaacagta catacagaca atggcagcaa tttcaccagt actacagtta
     4441 aggccgcctg ttggtgggcg gggatcaagc aggaatttgg cattccctac aatccccaaa
     4501 gtcaaggagt aatagaatct atgaataaag aattaaagaa aattatagga caggtaagag
     4561 atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac aattttaaaa
     4621 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4681 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4741 acagggacag cagagatcca gtttggaaag gaccagcaaa gctcctctgg aaaggtgaag
     4801 gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga aaagcaaaga
     4861 tcatcaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt agacaggatg
     4921 aggattaaca catggaaaag attagtaaaa caccatatgt atatttcaag gaaagctaag
     4981 gactggtttt atagacatca ctatgaaagt actaatccaa aaataagttc agaagtacac
     5041 atcccactag gggatgctaa attagtaata acaacatatt ggggtctgca tacaggagaa
     5101 agagactggc atttgggtca gggagtctcc atagaatgga ggaaaaagag atatagcaca
     5161 caagtagacc ctgacctagc agaccaacta attcatctgc actattttga ttgtttttca
     5221 gaatctgcta taagaaatac catattagga cgtatagtta gtcctaggtg tgaatatcaa
     5281 gcaggacata acaaggtagg atctctacag tacttggcac tagcagcatt aataaaacca
     5341 aaacagataa agccaccttt gcctagtgtt aggaaactga cagaggacag atggaacaag
     5401 ccccagaaga ccaagggcca cagagggagc catacaatga atggacacta gagcttttag
     5461 aggaacttaa ttaagagtga agctgttaga cattttccta ggatatggct ccataactta
     5521 ggacaacata tctatgaaac ttacggggat acttgggcag gagtggaagc cataataaga
     5581 attctgcaac aactgctgtt tatccatttc agaattgggt gtcgacatag cagaataggc
     5641 gttactcgac agaggagagc aagaaatgga gccagtagat cctagactag agccctggaa
     5701 gcatccagga agtcagccta aaactgcttg taccaattgc tattgtaaaa agtgttgctt
     5761 tcattgccaa gtttgtttca tgacaaaagc cttaggcatc tcctatggca ggaagaagcg
     5821 gagacagcga cgaagagctc atcagaacag tcagactcat caagcttctc tatcaaagca
     5881 gtaagtagta catgtaatgc aacctataat agtagcaata gtagcattag tagtagcaat
     5941 aataatagca atagttgtgt ggtccatagt aatcatagaa tataggaaaa tattaagaca
     6001 aagaaaaata gacaggttaa ttgatagact aatagaaaga gcagaagaca gtggcaatga
     6061 gagtgaagga gaagtatcag cacttgtgga gatgggggtg gaaatggggc accatgctcc
     6121 ttgggatatt gatgatctgt agtgcttagt aaggatgttc atcaaatatt actgggctgc
     6181 tattaacaag agatggtggt aataacaaca atgggtccga gatcttcaga cctggaggag
     6241 gcgatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6301 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6361 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6421 cgtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg cagcagcaga
     6481 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     6541 tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     6601 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     6661 gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg gagtgggaca
     6721 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     6781 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     6841 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     6901 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     6961 caccattatc gtttcagacc cacctcccaa tcccgagggg acccgacagg cccgaaggaa
     7021 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcct
     7081 tagcacttat ctgggacgat ctgcggagcc tgtgcctctt cagctaccac cgcttgagag
     7141 acttactctt gattgtaacg aggattgtgg aacttctggg acgcaggggg tgggaagccc
     7201 tcaaatattg gtggaatctc ctacaatatt ggagtcagga gctaaagaat agtgctgtta
     7261 gcttgctcaa tgccacagcc atagcagtag ctgaggggac agatagggtt atagaagtag
     7321 tacaaggagc ttgtagagct attcgccaca tacctagaag aataagacag ggcttggaaa
     7381 ggattttgct ataagatggg tggcgcggcc gctgatagca ctgagaacgt catcaagccc
     7441 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     7501 ggcgtgggcg agggcaagcc ctacgagggc acccagaccg ccaagctgca agtgaccaag
     7561 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcttcta cggctccaag
     7621 gcgtacatca agcaccccgc cgacatcccc gactacctca agcagtcctt ccccgagggc
     7681 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     7741 tcctctctgc aggacggcac cctcatctac cacgtgaagt tcatcggcgt gaacttcccc
     7801 tccgacggcc ccgtaatgca gaagaagact ctgggctggg agccctccac tgagcgcaac
     7861 tacccccgcg acggcgtgct gaagggcgag aaccacatgg cgctgaagct gaagggcggc
     7921 ggccactacc tgtgtgagtt caagtccatc tacatggcca agaagcccgt gaagctgccc
     7981 ggctaccact acgtggacta caagctcgac atcacctccc acaacgagga ctacaccgtg
     8041 gtggagcagt acgagcgcgc cgaggcccgc caccacctgt tccagtagcc tgcagacggc
     8101 cgcggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     8161 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     8221 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     8281 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     8341 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     8401 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     8461 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     8521 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     8581 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
     8641 cgcctacgct agcgctaccg gtcgtacgcg ccaccatggc ccagtccaag cacggcctga
     8701 ccaaggagat gaccatgaag taccgcatgg agggctgcgt ggacggccac aagttcgtga
     8761 tcaccggcga gggcatcggc taccccttca agggcaagca ggccatcaac ctgtgcgtgg
     8821 tggagggcgg ccccttgccc ttcgccgagg acatcttgtc cgccgccttc atgtacggca
     8881 accgcgtgtt caccgagtac ccccaggaca tcgtcgacta cttcaagaac tcctgccccg
     8941 ccggctacac ctgggaccgc tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg
     9001 ccgacatcac cgtgagcgtg gaggagaact gcatgtacca cgagtccaag ttctacggcg
     9061 tgaacttccc cgccgacggc cccgtgatga agaagatgac cgacaactgg gagccctcct
     9121 gcgagaagat catccccgtg cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc
     9181 tgctgctgaa ggacggtggc cgcttgcgct gccagttcga caccgtgtac aaggccaagt
     9241 ccgtgccccg caagatgccc gactggcact tcatccagca caagctgacc cgcgaggacc
     9301 gcagcgacgc caagaaccag aagtggcacc tgaccgagca cgccatcgcc tccggctccg
     9361 ccttgcccgg aagtggggcg accaacttct ctttgcttaa acaggctggt gacgttgaag
     9421 agaatcccgg gccgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     9481 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     9541 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     9601 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     9661 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     9721 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     9781 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     9841 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     9901 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     9961 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
    10021 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
    10081 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
    10141 agctgtacaa gtaactcgag acctagaaaa acatggagca atcacaagta gcaatacagc
    10201 agctaacaat gctgcttgtg cctggctaga agcacaagag gaggaagagg tgggttttcc
    10261 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    10321 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatat
    10381 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
    10441 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    10501 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
    10561 cctgcatgga atggatgacc ctgagagaga agtgttagag tggaggtttg acagccgcct
    10621 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgacatcg
    10681 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
    10741 tggggagtgg cgagccctca gatgctgcat ataagcagct gctttttgcc tgtactgggt
    10801 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10861 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10921 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaccc
    10981 aggaggtaga ggttgcagtg agccaagatc gcgccactgc attccagcct gggcaagaaa
    11041 acaagactgt ctaaaataat aataataagt taagggtatt aaatatattt atacatggag
    11101 gtcataaaaa tatatatatt tgggctgggc gcagtggctc acacctgcgc ccggcccttt
    11161 gggaggccga ggcaggtgga tcacctgagt ttgggagttc cagaccagcc tgaccaacat
    11221 ggagaaaccc cttctctgtg tatttttagt agattttatt ttatgtgtat tttattcaca
    11281 ggtatttctg gaaaactgaa actgtttttc ctctactctg ataccacaag aatcatcagc
    11341 acagaggaag acttctgtga tcaaatgtgg tgggagaggg aggttttcac cagcacatga
    11401 gcagtcagtt ctgccgcaga ctcggcgggt gtccttcggt tcagttccaa caccgcctgc
    11461 ctggagagag gtcagaccac agggtgaggg ctcagtcccc aagacataaa cacccaagac
    11521 ataaacaccc aacaggtcca ccccgcctgc tgcccaggca gagccgattc accaagacgg
    11581 gaattaggat agagaaagag taagtcacac agagccggct gtgcgggaga acggagttct
    11641 attatgactc aaatcagtct ccccaagcat tcggggatca gagtttttaa ggataactta
    11701 gtgtgtaggg ggccagtgag ttggagatga aagcgtaggg agtcgaaggt gtccttttgc
    11761 gccgagtcag ttcctgggtg ggggccacaa gatcggatga gccagtttat caatccgggg
    11821 gtgccagctg atccatggag tgcagggtct gcaaaatatc tcaagcactg attgatctta
    11881 ggttttacaa tagtgatgtt accccaggaa caatttgggg aaggtcagaa tcttgtagcc
    11941 tgtagctgca tgactcctaa accataattt cttttttgtt tttttttttt tatttttgag
    12001 acagggtctc actctgtcac ctaggctgga gtgcagtggt gcaatcacag ctcactgcag
    12061 cctcaacgtc gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag ctgagactac
    12121 aagcgacgcc ccagttaatt tttgtatttt tggtagaggc agcgttttgc cgtgtggccc
    12181 tggctggtct cgaactcctg ggctcaagtg atccagcctc agcctcccaa agtgctggga
    12241 caaccggggc cagtcactgc acctggccct aaaccataat ttctaatctt ttggctaatt
    12301 tgttagtcct acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt tcgggaaagg
    12361 gctgttactg tctttgtttc aaactataaa ctaagttcct cctaaactta gttcggccta
    12421 cacccaggaa tgaacaagga gagcttggag gttagaagca cgatggaatt ggttaggtca
    12481 gatctctttc actgtctgag ttataatttt gcaatggtgg ttcaaagact gcccgcttct
    12541 gacaccagtc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    12601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    12661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    12721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    12781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    12841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    12901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    12961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    13021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    13081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    13141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    13201 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    13261 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    13321 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    13381 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    13441 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    13501 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    13561 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    13621 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    13681 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    13741 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    13801 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    13861 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    13921 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    13981 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    14041 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    14101 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    14161 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    14221 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    14281 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    14341 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    14401 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    14461 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    14521 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    14581 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    14641 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    14701 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    14761 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    14821 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    14881 ggcgatcggt gcgggcctct tcgctattac gccaggggag gcagagattg cagtaagctg
    14941 agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa
    15001 taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt
    15061 ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct
    15121 ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca
    15181 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac
    15241 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata
    15301 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg
    15361 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag
    15421 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc
    15481 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg
    15541 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca
    15601 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg
    15661 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta
    15721 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta
    15781 tatacagggt tcagtactat cgcatttcag gcctccacct gggtcttgga atgtgtcccc
    15841 cgaggggtga tgactacctc agttggatct ccacaggtca cagtgacaca agataaccaa
    15901 gacacctccc aaggctacca caatgggccg ccctccacgt gcacatggcc ggaggaactg
    15961 ccatgtcgga ggtgcaagca cacctgcgca tcagagtcct tggtgtggag ggagggacca
    16021 gcgcagcttc cagccatcca cctgatgaac agaacctagg gaaagcccca gttctactta
    16081 caccaggaaa ggctggaagg gctaatttgg tcccaaaaaa gacaagagat ccttgatctg
    16141 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
    16201 atcagatatc cactgacctt tggatggtgc ttcaagttag taccagttga accagagcaa
    16261 g
//
