LOCUS       sequence-379936-       15878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monitor HIV-1 fate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15878)
  AUTHORS   Ratnapriya S, Harris M, Chov A, Herbert ZT, Vrbanac V, Deruaz M, 
            Achuthan V, Engelman AN, Sodroski J, Herschhorn A
  TITLE     Intra- and extra-cellular environments contribute to the fate of 
            HIV-1 infection.
  JOURNAL   Cell Rep. 2021 Aug 31;36(9):109622. doi: 
            10.1016/j.celrep.2021.109622.
  PUBMED    34469717
REFERENCE   2  (bases 1 to 15878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-08-31"; volume: "36(9):109622. doi"; pages: " 
            10.1016/j.celrep.2021.109622"
COMMENT     Sequence Label: HI.fate.SFFV-E
FEATURES             Location/Qualifiers
     source          1..15878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             546..803
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             959..2461
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2254..5265
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4950..5067
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6705..6938
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7123..7167
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7461..7487
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7751..8425
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHMA
                     LKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     8146..8165
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8443..8844
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     promoter        8877..9088
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9101..9369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      9407..9416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9413..10108
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     LTR             10298..10931
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12571..12588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12742..13330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12822..12841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13501..14292,14293..14361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14124..14143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14362..14466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14534..14552
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14590..14612)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14712..14731
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccgccct ccacgtgcac atggccggag gaactgccat gtcggaggtg caagcacacc
       61 tgcgcatcag agtccttggt gtggagggag ggaccagcgc agcttccagc catccacctg
      121 atgaacagaa cctagggaaa gccccagttc tacttacacc aggaaaggct ggaagggcta
      181 atttggtccc aaaaaagaca agagatcctt gatctgtgga tctaccacac acaaggctac
      241 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
      301 tggtgcttca agttagtacc agttgaacca gagcaagtag aagaggccaa tgaaggagag
      361 aacaacagct tgttacaccc tatgagccag catgggatgg aggacccgga gggagaagta
      421 ttagtgtgga agtttgacag cctcctagca tttcgtcaca tggcccgaga gctgcatccg
      481 gagtactaca aagactgctg acatcgagct ttctacaagg gactttccgc tggggacttt
      541 ccagggaggt gtggcctggg cgggactggg gagtggcgag ccctcagatg ctacatataa
      601 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgctcaa
      721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      781 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagtaaag
      841 ccagaggaga tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      901 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      961 gggtgcgaga gcgtcggtat taagcggggg agaattagat aaatgggaaa aaattcggtt
     1021 aaggccaggg ggaaagaaac aatataaact aaaacatata gtatgggcaa gcagggagct
     1081 agaacgattc gcagttaatc ctggcctttt agagacatca gaaggctgta gacaaatact
     1141 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1201 aatagcagtc ctctattgtg tgcatcaaag gatagatgta aaagacacca aggaagcctt
     1261 agataagata gaggaagagc aaaacaaaag taagaaaaag gcacagcaag cagcagctga
     1321 cacaggaaac aacagccagg tcagccaaaa ttaccctata gtgcagaacc tccaggggca
     1381 aatggtacat caggccatat cacctagaac tttaaatgca tgggtaaaag tagtagaaga
     1441 gaaggctttc agcccagaag taatacccat gttttcagca ttatcagaag gagccacccc
     1501 acaagattta aataccatgc taaacacagt ggggggacat caagcagcca tgcaaatgtt
     1561 aaaagagacc atcaatgagg aagctgcaga atgggataga ttgcatccag tgcatgcagg
     1621 gcctattgca ccaggccaga tgagagaacc aaggggaagt gacatagcag gaactactag
     1681 tacccttcag gaacaaatag gatggatgac acataatcca cctatcccag taggagaaat
     1741 ctataaaaga tggataatcc tgggattaaa taaaatagta agaatgtata gccctaccag
     1801 cattctggac ataagacaag gaccaaagga accctttaga gactatgtag accgattcta
     1861 taaaactcta agagccgagc aagcttcaca agaggtaaaa aattggatga cagaaacctt
     1921 gttggtccaa aatgcgaacc cagattgtaa gactatttta aaagcattgg gaccaggagc
     1981 gacactagaa gaaatgatga cagcatgtca gggagtgggg ggacccggcc ataaagcaag
     2041 agttttggct gaagcaatga gccaagtaac aaatccagct accataatga tacagaaagg
     2101 caattttagg aaccaaagaa agactgttaa gtgtttcaat tgtggcaaag aagggcacat
     2161 agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg aaatgtggaa aggaaggaca
     2221 ccaaatgaaa gattgtactg agagacaggc taatttttta gggaagatct ggccttccca
     2281 caagggaagg ccagggaatt ttcttcagag cagaccagag ccaacagccc caccagaaga
     2341 gagcttcagg tttggggaag agacaacaac tccctctcag aagcaggagc cgatagacaa
     2401 ggaactgtat cctttagctt ccctcagatc actctttggc agcgacccct cgtcacaata
     2461 aagatagggg ggcaattaaa ggaagctcta ttagatacag gagcagatga tacagtatta
     2521 gaagaaatga atttgccagg aagatggaaa ccaaaaatga tagggggaat tggaggtttt
     2581 atcaaagtaa gacagtatga tcagatactc atagaaatct gcggacataa agctataggt
     2641 acagtattag taggacctac acctgtcaac ataattggaa gaaatctgtt gactcagatt
     2701 ggctgcactt taaattttcc cattagtcct attgagactg taccagtaaa attaaagcca
     2761 ggaatggatg gcccaaaagt taaacaatgg ccattgacag aagaaaaaat aaaagcatta
     2821 gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt caaaaattgg gcctgaaaat
     2881 ccatacaata ctccagtatt tgccataaag aaaaaagaca gtactaaatg gagaaaatta
     2941 gtagatttca gagaacttaa taagagaact caagatttct gggaagttca attaggaata
     3001 ccacatcctg cagggttaaa acagaaaaaa tcagtaacag tactggatgt gggcgatgca
     3061 tatttttcag ttcccttaga taaagacttc aggaagtata ctgcatttac catacctagt
     3121 ataaacaatg agacaccagg gattagatat cagtacaatg tgcttccaca gggatggaaa
     3181 ggatcaccag caatattcca gtgtagcatg acaaaaatct tagagccttt tagaaaacaa
     3241 aatccagaca tagtcatcta tcaatacatg gatgatttgt atgtaggatc tgacttagaa
     3301 atagggcagc atagaacaaa aatagaggaa ctgagacaac atctgttgag gtggggattt
     3361 accacaccag acaaaaaaca tcagaaagaa cctccattcc tttggatggg ttatgaactc
     3421 catcctgata aatggacagt acagcctata gtgctgccag aaaaggacag ctggactgtc
     3481 aatgacatac agaaattagt gggaaaattg aattgggcaa gtcagattta tgcagggatt
     3541 aaagtaaggc aattatgtaa acttcttagg ggaaccaaag cactaacaga agtagtacca
     3601 ctaacagaag aagcagagct agaactggca gaaaacaggg agattctaaa agaaccggta
     3661 catggagtgt attatgaccc atcaaaagac ttaatagcag aaatacagaa gcaggggcaa
     3721 ggccaatgga catatcaaat ttatcaagag ccatttaaaa atctgaaaac aggaaagtat
     3781 gcaagaatga agggtgccca cactaatgat gtgaaacaat taacagaggc agtacaaaaa
     3841 atagccacag aaagcatagt aatatgggga aagactccta aatttaaatt acccatacaa
     3901 aaggaaacat gggaagcatg gtggacagag tattggcaag ccacctggat tcctgagtgg
     3961 gagtttgtca atacccctcc cttagtgaag ttatggtacc agttagagaa agaacccata
     4021 ataggagcag aaactttcta tgtagatggg gcagccaata gggaaactaa attaggaaaa
     4081 gcaggatatg taactgacag aggaagacaa aaagttgtcc ccctaacgga cacaacaaat
     4141 cagaagactg agttacaagc aattcatcta gctttgcagg attcgggatt agaagtaaac
     4201 atagtgacag actcacaata tgcattggga atcattcaag cacaaccaga taagagtgaa
     4261 tcagagttag tcagtcaaat aatagagcag ttaataaaaa aggaaaaagt ctacctggca
     4321 tgggtaccag cacacaaagg aattggagga aatgaacaag tagataaatt ggtcagtgct
     4381 ggaatcagga aagtactatt tttagatgga atagataagg cccaagaaga acatgagaaa
     4441 tatcacagta attggagagc aatggctagt gattttaacc taccacctgt agtagcaaaa
     4501 gaaatagtag ccagctgtga taaatgtcag ctaaaagggg aagccatgca tggacaagta
     4561 gactgtagcc caggaatatg gcagctagat tgtacacatt tagaaggaaa agttatcttg
     4621 gtagcagttc atgtagccag tggatatata gaagcagaag taattccagc agagacaggg
     4681 caagaaacag catacttcct cttaaaatta gcaggaagat ggccagtaaa aacagtacat
     4741 acagacaatg gcagcaattt caccagtact acagttaagg ccgcctgttg gtgggcgggg
     4801 atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtaat agaatctatg
     4861 aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca tcttaagaca
     4921 gcagtacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     4981 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     5041 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     5101 tggaaaggac cagcaaagct cctctggaaa ggtgaagggg cagtagtaat acaagataat
     5161 agtgacataa aagtagtgcc aagaagaaaa gcaaagatca tcagggatta tggaaaacag
     5221 atggcaggtg atgattgtgt ggcaagtaga caggatgagg attaacacat ggaaaagatt
     5281 agtaaaacac catatgtata tttcaaggaa agctaaggac tggttttata gacatcacta
     5341 tgaaagtact aatccaaaaa taagttcaga agtacacatc ccactagggg atgctaaatt
     5401 agtaataaca acatattggg gtctgcatac aggagaaaga gactggcatt tgggtcaggg
     5461 agtctccata gaatggagga aaaagagata tagcacacaa gtagaccctg acctagcaga
     5521 ccaactaatt catctgcact attttgattg tttttcagaa tctgctataa gaaataccat
     5581 attaggacgt atagttagtc ctaggtgtga atatcaagca ggacataaca aggtaggatc
     5641 tctacagtac ttggcactag cagcattaat aaaaccaaaa cagataaagc cacctttgcc
     5701 tagtgttagg aaactgacag aggacagatg gaacaagccc cagaagacca agggccacag
     5761 agggagccat acaatgaatg gacactagag cttttagagg aacttaatta agagtgaagc
     5821 tgttagacat tttcctagga tatggctcca taacttagga caacatatct atgaaactta
     5881 cggggatact tgggcaggag tggaagccat aataagaatt ctgcaacaac tgctgtttat
     5941 ccatttcaga attgggtgtc gacatagcag aataggcgtt actcgacaga ggagagcaag
     6001 aaatggagcc agtagatcct agactagagc cctggaagca tccaggaagt cagcctaaaa
     6061 ctgcttgtac caattgctat tgtaaaaagt gttgctttca ttgccaagtt tgtttcatga
     6121 caaaagcctt aggcatctcc tatggcagga agaagcggag acagcgacga agagctcatc
     6181 agaacagtca gactcatcaa gcttctctat caaagcagta agtagtacat gtaatgcaac
     6241 ctataatagt agcaatagta gcattagtag tagcaataat aatagcaata gttgtgtggt
     6301 ccatagtaat catagaatat aggaaaatat taagacaaag aaaaatagac aggttaattg
     6361 atagactaat agaaagagca gaagacagtg gcaatgagag tgaaggagaa gtatcagcac
     6421 ttgtggagat gggggtggaa atggggcacc atgctccttg ggatattgat gatctgtagt
     6481 gcttagtaag gatgttcatc aaatattact gggctgctat taacaagaga tggtggtaat
     6541 aacaacaatg ggtccgagat cttcagacct ggaggaggcg atatgaggga caattggaga
     6601 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6661 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6721 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6781 gccagacaat tattgtctga tatagtgcag cagcagaaca atttgctgag ggctattgag
     6841 gcgcaacagc atctgttgca actcacagtc tggggcatca aacagctcca ggcaagaatc
     6901 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6961 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7021 cagatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     7081 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7141 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7201 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7261 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7321 ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7381 gacagagaca gatccattcg attagtgaac ggatccttag cacttatctg ggacgatctg
     7441 cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg
     7501 attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta
     7561 caatattgga gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagccata
     7621 gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt
     7681 cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgctata agatgggtgg
     7741 cgcggccgct gatagcactg agaacgtcat caagcccttc atgcgcttca aggtgcacat
     7801 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gtgggcgagg gcaagcccta
     7861 cgagggcacc cagaccgcca agctgcaagt gaccaagggc ggccccctgc ccttcgcctg
     7921 ggacatcctg tccccccagt tcttctacgg ctccaaggcg tacatcaagc accccgccga
     7981 catccccgac tacctcaagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     8041 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tctctgcagg acggcaccct
     8101 catctaccac gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
     8161 gaagactctg ggctgggagc cctccactga gcgcaactac ccccgcgacg gcgtgctgaa
     8221 gggcgagaac cacatggcgc tgaagctgaa gggcggcggc cactacctgt gtgagttcaa
     8281 gtccatctac atggccaaga agcccgtgaa gctgcccggc taccactacg tggactacaa
     8341 gctcgacatc acctcccaca acgaggacta caccgtggtg gagcagtacg agcgcgccga
     8401 ggcccgccac cacctgttcc agtagcctgc agacggccgc gggtaacgcc attttgcaag
     8461 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     8521 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     8581 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     8641 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     8701 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     8761 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     8821 ccagtcctcc gacagactga gtcggcggat ctgcgatcgc tccggtgccc gtcagtgggc
     8881 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     8941 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     9001 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     9061 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     9121 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     9181 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     9241 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     9301 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     9361 ttacagatcc aagctgtgac cggcgcctac gctagcgcta ccggtcgcca ccatggccca
     9421 gtccaagcac ggcctgacca aggagatgac catgaagtac cgcatggagg gctgcgtgga
     9481 cggccacaag ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcaggc
     9541 catcaacctg tgcgtggtgg agggcggccc cttgcccttc gccgaggaca tcttgtccgc
     9601 cgccttcatg tacggcaacc gcgtgttcac cgagtacccc caggacatcg tcgactactt
     9661 caagaactcc tgccccgccg gctacacctg ggaccgctcc ttcctgttcg aggacggcgc
     9721 cgtgtgcatc tgcaacgccg acatcaccgt gagcgtggag gagaactgca tgtaccacga
     9781 gtccaagttc tacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccga
     9841 caactgggag ccctcctgcg agaagatcat ccccgtgccc aagcagggca tcttgaaggg
     9901 cgacgtgagc atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc agttcgacac
     9961 cgtgtacaag gccaagtccg tgccccgcaa gatgcccgac tggcacttca tccagcacaa
    10021 gctgacccgc gaggaccgca gcgacgccaa gaaccagaag tggcacctga ccgagcacgc
    10081 catcgcctcc ggctccgcct tgccctagct cgagacctag aaaaacatgg agcaatcaca
    10141 agtagcaata cagcagctaa caatgctgct tgtgcctggc tagaagcaca agaggaggaa
    10201 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
    10261 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
    10321 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
    10381 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10441 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10501 ttacaccctg tgagcctgca tggaatggat gaccctgaga gagaagtgtt agagtggagg
    10561 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10621 aactgctgac atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10681 ggcctgggcg ggactgggga gtggcgagcc ctcagatgct gcatataagc agctgctttt
    10741 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10801 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10861 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10921 aatctctagc acccaggagg tagaggttgc agtgagccaa gatcgcgcca ctgcattcca
    10981 gcctgggcaa gaaaacaaga ctgtctaaaa taataataat aagttaaggg tattaaatat
    11041 atttatacat ggaggtcata aaaatatata tatttgggct gggcgcagtg gctcacacct
    11101 gcgcccggcc ctttgggagg ccgaggcagg tggatcacct gagtttggga gttccagacc
    11161 agcctgacca acatggagaa accccttctc tgtgtatttt tagtagattt tattttatgt
    11221 gtattttatt cacaggtatt tctggaaaac tgaaactgtt tttcctctac tctgatacca
    11281 caagaatcat cagcacagag gaagacttct gtgatcaaat gtggtgggag agggaggttt
    11341 tcaccagcac atgagcagtc agttctgccg cagactcggc gggtgtcctt cggttcagtt
    11401 ccaacaccgc ctgcctggag agaggtcaga ccacagggtg agggctcagt ccccaagaca
    11461 taaacaccca agacataaac acccaacagg tccaccccgc ctgctgccca ggcagagccg
    11521 attcaccaag acgggaatta ggatagagaa agagtaagtc acacagagcc ggctgtgcgg
    11581 gagaacggag ttctattatg actcaaatca gtctccccaa gcattcgggg atcagagttt
    11641 ttaaggataa cttagtgtgt agggggccag tgagttggag atgaaagcgt agggagtcga
    11701 aggtgtcctt ttgcgccgag tcagttcctg ggtgggggcc acaagatcgg atgagccagt
    11761 ttatcaatcc gggggtgcca gctgatccat ggagtgcagg gtctgcaaaa tatctcaagc
    11821 actgattgat cttaggtttt acaatagtga tgttacccca ggaacaattt ggggaaggtc
    11881 agaatcttgt agcctgtagc tgcatgactc ctaaaccata atttcttttt tgtttttttt
    11941 tttttatttt tgagacaggg tctcactctg tcacctaggc tggagtgcag tggtgcaatc
    12001 acagctcact gcagcctcaa cgtcgtaagc tcaagcgatc ctcccacctc agcctgcctg
    12061 gtagctgaga ctacaagcga cgccccagtt aatttttgta tttttggtag aggcagcgtt
    12121 ttgccgtgtg gccctggctg gtctcgaact cctgggctca agtgatccag cctcagcctc
    12181 ccaaagtgct gggacaaccg gggccagtca ctgcacctgg ccctaaacca taatttctaa
    12241 tcttttggct aatttgttag tcctacaaag gcagtctagt ccccaggcaa aaagggggtt
    12301 tgtttcggga aagggctgtt actgtctttg tttcaaacta taaactaagt tcctcctaaa
    12361 cttagttcgg cctacaccca ggaatgaaca aggagagctt ggaggttaga agcacgatgg
    12421 aattggttag gtcagatctc tttcactgtc tgagttataa ttttgcaatg gtggttcaaa
    12481 gactgcccgc ttctgacacc agtcgctgca ttaatgaatc ggccaacgcg cggggagagg
    12541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    12601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    12661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    12721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    12781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    12841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    12901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    12961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    13021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    13081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    13141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    13201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    13261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    13321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    13381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    13441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    13501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    13561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    13621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    13681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    13741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    13801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    13861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    13921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    13981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    14041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    14101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    14161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    14221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    14281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    14341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    14401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    14461 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    14521 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    14581 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    14641 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    14701 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    14761 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    14821 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagg ggaggcagag
    14881 attgcagtaa gctgagatcg cagcactgca ctccagcctg ggcgacagag taagactctg
    14941 tctcaaaaat aaaataaata aatcaatcag atattccaat cttttccttt atttatttat
    15001 ttattttcta ttttggaaac acagtccttc cttattccag aattacacat atattctatt
    15061 tttctttata tgctccagtt ttttttagac cttcacctga aatgtgtgta tacaaaatct
    15121 aggccagtcc agcagagcct aaaggtaaaa aataaaataa taaaaaataa ataaaatcta
    15181 gctcactcct tcacatcaaa atggagatac agctgttagc attaaatacc aaataaccca
    15241 tcttgtcctc aataatttta agcgcctctc tccaccacat ctaactcctg tcaaaggcat
    15301 gtgccccttc cgggcgctct gctgtgctgc caaccaactg gcatgtggac tctgcagggt
    15361 ccctaactgc caagccccac agtgtgccct gaggctgccc cttccttcta gcggctgccc
    15421 ccactcggct ttgctttccc tagtttcagt tacttgcgtt cagccaaggt ctgaaactag
    15481 gtgcgcacag agcggtaaga ctgcgagaga aagagaccag ctttacaggg ggtttatcac
    15541 agtgcaccct gacagtcgtc agcctcacag ggggtttatc acattgcacc ctgacagtcg
    15601 tcagcctcac agggggttta tcacagtgca cccttacaat cattccattt gattcacaat
    15661 ttttttagtc tctactgtgc ctaacttgta agttaaattt gatcagaggt gtgttcccag
    15721 aggggaaaac agtatataca gggttcagta ctatcgcatt tcaggcctcc acctgggtct
    15781 tggaatgtgt cccccgaggg gtgatgacta cctcagttgg atctccacag gtcacagtga
    15841 cacaagataa ccaagacacc tcccaaggct accacaat
//
