LOCUS       sequence-379940-       16126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monitor HIV-1 fate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16126)
  AUTHORS   Ratnapriya S, Harris M, Chov A, Herbert ZT, Vrbanac V, Deruaz M, 
            Achuthan V, Engelman AN, Sodroski J, Herschhorn A
  TITLE     Intra- and extra-cellular environments contribute to the fate of 
            HIV-1 infection.
  JOURNAL   Cell Rep. 2021 Aug 31;36(9):109622. doi: 
            10.1016/j.celrep.2021.109622.
  PUBMED    34469717
REFERENCE   2  (bases 1 to 16126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-08-31"; volume: "36(9):109622. doi"; pages: " 
            10.1016/j.celrep.2021.109622"
COMMENT     Sequence Label: HI.fate.UB
FEATURES             Location/Qualifiers
     source          1..16126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             496..753
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             909..2411
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2204..5215
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4900..5017
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6655..6888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7073..7117
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7411..7437
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7701..8375
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHMA
                     LKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     8096..8115
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8386..9597
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9434..9453
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      9605..9614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9611..10306
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     LTR             10496..11129
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12769..12786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12940..13528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13020..13039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13699..14490,14491..14559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14322..14341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14560..14664)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14732..14750
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14788..14810)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14910..14929
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caagcacacc tgcgcatcag agtccttggt gtggagggag ggaccagcgc agcttccagc
       61 catccacctg atgaacagaa cctagggaaa gccccagttc tacttacacc aggaaaggct
      121 ggaagggcta atttggtccc aaaaaagaca agagatcctt gatctgtgga tctaccacac
      181 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
      241 gacctttgga tggtgcttca agttagtacc agttgaacca gagcaagtag aagaggccaa
      301 tgaaggagag aacaacagct tgttacaccc tatgagccag catgggatgg aggacccgga
      361 gggagaagta ttagtgtgga agtttgacag cctcctagca tttcgtcaca tggcccgaga
      421 gctgcatccg gagtactaca aagactgctg acatcgagct ttctacaagg gactttccgc
      481 tggggacttt ccagggaggt gtggcctggg cgggactggg gagtggcgag ccctcagatg
      541 ctacatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      601 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      661 gagtgctcaa agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      721 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      781 gaaagtaaag ccagaggaga tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      841 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      901 ggagagagat gggtgcgaga gcgtcggtat taagcggggg agaattagat aaatgggaaa
      961 aaattcggtt aaggccaggg ggaaagaaac aatataaact aaaacatata gtatgggcaa
     1021 gcagggagct agaacgattc gcagttaatc ctggcctttt agagacatca gaaggctgta
     1081 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1141 tatataatac aatagcagtc ctctattgtg tgcatcaaag gatagatgta aaagacacca
     1201 aggaagcctt agataagata gaggaagagc aaaacaaaag taagaaaaag gcacagcaag
     1261 cagcagctga cacaggaaac aacagccagg tcagccaaaa ttaccctata gtgcagaacc
     1321 tccaggggca aatggtacat caggccatat cacctagaac tttaaatgca tgggtaaaag
     1381 tagtagaaga gaaggctttc agcccagaag taatacccat gttttcagca ttatcagaag
     1441 gagccacccc acaagattta aataccatgc taaacacagt ggggggacat caagcagcca
     1501 tgcaaatgtt aaaagagacc atcaatgagg aagctgcaga atgggataga ttgcatccag
     1561 tgcatgcagg gcctattgca ccaggccaga tgagagaacc aaggggaagt gacatagcag
     1621 gaactactag tacccttcag gaacaaatag gatggatgac acataatcca cctatcccag
     1681 taggagaaat ctataaaaga tggataatcc tgggattaaa taaaatagta agaatgtata
     1741 gccctaccag cattctggac ataagacaag gaccaaagga accctttaga gactatgtag
     1801 accgattcta taaaactcta agagccgagc aagcttcaca agaggtaaaa aattggatga
     1861 cagaaacctt gttggtccaa aatgcgaacc cagattgtaa gactatttta aaagcattgg
     1921 gaccaggagc gacactagaa gaaatgatga cagcatgtca gggagtgggg ggacccggcc
     1981 ataaagcaag agttttggct gaagcaatga gccaagtaac aaatccagct accataatga
     2041 tacagaaagg caattttagg aaccaaagaa agactgttaa gtgtttcaat tgtggcaaag
     2101 aagggcacat agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg aaatgtggaa
     2161 aggaaggaca ccaaatgaaa gattgtactg agagacaggc taatttttta gggaagatct
     2221 ggccttccca caagggaagg ccagggaatt ttcttcagag cagaccagag ccaacagccc
     2281 caccagaaga gagcttcagg tttggggaag agacaacaac tccctctcag aagcaggagc
     2341 cgatagacaa ggaactgtat cctttagctt ccctcagatc actctttggc agcgacccct
     2401 cgtcacaata aagatagggg ggcaattaaa ggaagctcta ttagatacag gagcagatga
     2461 tacagtatta gaagaaatga atttgccagg aagatggaaa ccaaaaatga tagggggaat
     2521 tggaggtttt atcaaagtaa gacagtatga tcagatactc atagaaatct gcggacataa
     2581 agctataggt acagtattag taggacctac acctgtcaac ataattggaa gaaatctgtt
     2641 gactcagatt ggctgcactt taaattttcc cattagtcct attgagactg taccagtaaa
     2701 attaaagcca ggaatggatg gcccaaaagt taaacaatgg ccattgacag aagaaaaaat
     2761 aaaagcatta gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt caaaaattgg
     2821 gcctgaaaat ccatacaata ctccagtatt tgccataaag aaaaaagaca gtactaaatg
     2881 gagaaaatta gtagatttca gagaacttaa taagagaact caagatttct gggaagttca
     2941 attaggaata ccacatcctg cagggttaaa acagaaaaaa tcagtaacag tactggatgt
     3001 gggcgatgca tatttttcag ttcccttaga taaagacttc aggaagtata ctgcatttac
     3061 catacctagt ataaacaatg agacaccagg gattagatat cagtacaatg tgcttccaca
     3121 gggatggaaa ggatcaccag caatattcca gtgtagcatg acaaaaatct tagagccttt
     3181 tagaaaacaa aatccagaca tagtcatcta tcaatacatg gatgatttgt atgtaggatc
     3241 tgacttagaa atagggcagc atagaacaaa aatagaggaa ctgagacaac atctgttgag
     3301 gtggggattt accacaccag acaaaaaaca tcagaaagaa cctccattcc tttggatggg
     3361 ttatgaactc catcctgata aatggacagt acagcctata gtgctgccag aaaaggacag
     3421 ctggactgtc aatgacatac agaaattagt gggaaaattg aattgggcaa gtcagattta
     3481 tgcagggatt aaagtaaggc aattatgtaa acttcttagg ggaaccaaag cactaacaga
     3541 agtagtacca ctaacagaag aagcagagct agaactggca gaaaacaggg agattctaaa
     3601 agaaccggta catggagtgt attatgaccc atcaaaagac ttaatagcag aaatacagaa
     3661 gcaggggcaa ggccaatgga catatcaaat ttatcaagag ccatttaaaa atctgaaaac
     3721 aggaaagtat gcaagaatga agggtgccca cactaatgat gtgaaacaat taacagaggc
     3781 agtacaaaaa atagccacag aaagcatagt aatatgggga aagactccta aatttaaatt
     3841 acccatacaa aaggaaacat gggaagcatg gtggacagag tattggcaag ccacctggat
     3901 tcctgagtgg gagtttgtca atacccctcc cttagtgaag ttatggtacc agttagagaa
     3961 agaacccata ataggagcag aaactttcta tgtagatggg gcagccaata gggaaactaa
     4021 attaggaaaa gcaggatatg taactgacag aggaagacaa aaagttgtcc ccctaacgga
     4081 cacaacaaat cagaagactg agttacaagc aattcatcta gctttgcagg attcgggatt
     4141 agaagtaaac atagtgacag actcacaata tgcattggga atcattcaag cacaaccaga
     4201 taagagtgaa tcagagttag tcagtcaaat aatagagcag ttaataaaaa aggaaaaagt
     4261 ctacctggca tgggtaccag cacacaaagg aattggagga aatgaacaag tagataaatt
     4321 ggtcagtgct ggaatcagga aagtactatt tttagatgga atagataagg cccaagaaga
     4381 acatgagaaa tatcacagta attggagagc aatggctagt gattttaacc taccacctgt
     4441 agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaagggg aagccatgca
     4501 tggacaagta gactgtagcc caggaatatg gcagctagat tgtacacatt tagaaggaaa
     4561 agttatcttg gtagcagttc atgtagccag tggatatata gaagcagaag taattccagc
     4621 agagacaggg caagaaacag catacttcct cttaaaatta gcaggaagat ggccagtaaa
     4681 aacagtacat acagacaatg gcagcaattt caccagtact acagttaagg ccgcctgttg
     4741 gtgggcgggg atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtaat
     4801 agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca
     4861 tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     4921 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4981 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5041 agatccagtt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg cagtagtaat
     5101 acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca tcagggatta
     5161 tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg attaacacat
     5221 ggaaaagatt agtaaaacac catatgtata tttcaaggaa agctaaggac tggttttata
     5281 gacatcacta tgaaagtact aatccaaaaa taagttcaga agtacacatc ccactagggg
     5341 atgctaaatt agtaataaca acatattggg gtctgcatac aggagaaaga gactggcatt
     5401 tgggtcaggg agtctccata gaatggagga aaaagagata tagcacacaa gtagaccctg
     5461 acctagcaga ccaactaatt catctgcact attttgattg tttttcagaa tctgctataa
     5521 gaaataccat attaggacgt atagttagtc ctaggtgtga atatcaagca ggacataaca
     5581 aggtaggatc tctacagtac ttggcactag cagcattaat aaaaccaaaa cagataaagc
     5641 cacctttgcc tagtgttagg aaactgacag aggacagatg gaacaagccc cagaagacca
     5701 agggccacag agggagccat acaatgaatg gacactagag cttttagagg aacttaatta
     5761 agagtgaagc tgttagacat tttcctagga tatggctcca taacttagga caacatatct
     5821 atgaaactta cggggatact tgggcaggag tggaagccat aataagaatt ctgcaacaac
     5881 tgctgtttat ccatttcaga attgggtgtc gacatagcag aataggcgtt actcgacaga
     5941 ggagagcaag aaatggagcc agtagatcct agactagagc cctggaagca tccaggaagt
     6001 cagcctaaaa ctgcttgtac caattgctat tgtaaaaagt gttgctttca ttgccaagtt
     6061 tgtttcatga caaaagcctt aggcatctcc tatggcagga agaagcggag acagcgacga
     6121 agagctcatc agaacagtca gactcatcaa gcttctctat caaagcagta agtagtacat
     6181 gtaatgcaac ctataatagt agcaatagta gcattagtag tagcaataat aatagcaata
     6241 gttgtgtggt ccatagtaat catagaatat aggaaaatat taagacaaag aaaaatagac
     6301 aggttaattg atagactaat agaaagagca gaagacagtg gcaatgagag tgaaggagaa
     6361 gtatcagcac ttgtggagat gggggtggaa atggggcacc atgctccttg ggatattgat
     6421 gatctgtagt gcttagtaag gatgttcatc aaatattact gggctgctat taacaagaga
     6481 tggtggtaat aacaacaatg ggtccgagat cttcagacct ggaggaggcg atatgaggga
     6541 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     6601 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     6661 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     6721 gacggtacag gccagacaat tattgtctga tatagtgcag cagcagaaca atttgctgag
     6781 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca aacagctcca
     6841 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     6901 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     6961 atctctggaa cagatttgga ataacatgac ctggatggag tgggacagag aaattaacaa
     7021 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7081 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7141 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7201 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     7261 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     7321 tggagagaga gacagagaca gatccattcg attagtgaac ggatccttag cacttatctg
     7381 ggacgatctg cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat
     7441 tgtaacgagg attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg
     7501 gaatctccta caatattgga gtcaggagct aaagaatagt gctgttagct tgctcaatgc
     7561 cacagccata gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg
     7621 tagagctatt cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgctata
     7681 agatgggtgg cgcggccgct gatagcactg agaacgtcat caagcccttc atgcgcttca
     7741 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gtgggcgagg
     7801 gcaagcccta cgagggcacc cagaccgcca agctgcaagt gaccaagggc ggccccctgc
     7861 ccttcgcctg ggacatcctg tccccccagt tcttctacgg ctccaaggcg tacatcaagc
     7921 accccgccga catccccgac tacctcaagc agtccttccc cgagggcttc aagtgggagc
     7981 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tctctgcagg
     8041 acggcaccct catctaccac gtgaagttca tcggcgtgaa cttcccctcc gacggccccg
     8101 taatgcagaa gaagactctg ggctgggagc cctccactga gcgcaactac ccccgcgacg
     8161 gcgtgctgaa gggcgagaac cacatggcgc tgaagctgaa gggcggcggc cactacctgt
     8221 gtgagttcaa gtccatctac atggccaaga agcccgtgaa gctgcccggc taccactacg
     8281 tggactacaa gctcgacatc acctcccaca acgaggacta caccgtggtg gagcagtacg
     8341 agcgcgccga ggcccgccac cacctgttcc agtagcctgc agacggccgc gggcgccggg
     8401 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     8461 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     8521 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     8581 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     8641 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     8701 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     8761 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     8821 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     8881 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     8941 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     9001 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     9061 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     9121 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     9181 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     9241 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     9301 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     9361 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     9421 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     9481 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     9541 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagaccgt
     9601 acgcgccacc atggcccagt ccaagcacgg cctgaccaag gagatgacca tgaagtaccg
     9661 catggagggc tgcgtggacg gccacaagtt cgtgatcacc ggcgagggca tcggctaccc
     9721 cttcaagggc aagcaggcca tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc
     9781 cgaggacatc ttgtccgccg ccttcatgta cggcaaccgc gtgttcaccg agtaccccca
     9841 ggacatcgtc gactacttca agaactcctg ccccgccggc tacacctggg accgctcctt
     9901 cctgttcgag gacggcgccg tgtgcatctg caacgccgac atcaccgtga gcgtggagga
     9961 gaactgcatg taccacgagt ccaagttcta cggcgtgaac ttccccgccg acggccccgt
    10021 gatgaagaag atgaccgaca actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa
    10081 gcagggcatc ttgaagggcg acgtgagcat gtacctgctg ctgaaggacg gtggccgctt
    10141 gcgctgccag ttcgacaccg tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg
    10201 gcacttcatc cagcacaagc tgacccgcga ggaccgcagc gacgccaaga accagaagtg
    10261 gcacctgacc gagcacgcca tcgcctccgg ctccgccttg ccctagctcg agacctagaa
    10321 aaacatggag caatcacaag tagcaataca gcagctaaca atgctgcttg tgcctggcta
    10381 gaagcacaag aggaggaaga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
    10441 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
    10501 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
    10561 ggctacttcc ctgattggca gaactacaca ccagggccag gggtcagata tccactgacc
    10621 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
    10681 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg gaatggatga ccctgagaga
    10741 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
    10801 catccggagt acttcaagaa ctgctgacat cgagcttgct acaagggact ttccgctggg
    10861 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatgctgc
    10921 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10981 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    11041 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    11101 cttttagtca gtgtggaaaa tctctagcac ccaggaggta gaggttgcag tgagccaaga
    11161 tcgcgccact gcattccagc ctgggcaaga aaacaagact gtctaaaata ataataataa
    11221 gttaagggta ttaaatatat ttatacatgg aggtcataaa aatatatata tttgggctgg
    11281 gcgcagtggc tcacacctgc gcccggccct ttgggaggcc gaggcaggtg gatcacctga
    11341 gtttgggagt tccagaccag cctgaccaac atggagaaac cccttctctg tgtattttta
    11401 gtagatttta ttttatgtgt attttattca caggtatttc tggaaaactg aaactgtttt
    11461 tcctctactc tgataccaca agaatcatca gcacagagga agacttctgt gatcaaatgt
    11521 ggtgggagag ggaggttttc accagcacat gagcagtcag ttctgccgca gactcggcgg
    11581 gtgtccttcg gttcagttcc aacaccgcct gcctggagag aggtcagacc acagggtgag
    11641 ggctcagtcc ccaagacata aacacccaag acataaacac ccaacaggtc caccccgcct
    11701 gctgcccagg cagagccgat tcaccaagac gggaattagg atagagaaag agtaagtcac
    11761 acagagccgg ctgtgcggga gaacggagtt ctattatgac tcaaatcagt ctccccaagc
    11821 attcggggat cagagttttt aaggataact tagtgtgtag ggggccagtg agttggagat
    11881 gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc agttcctggg tgggggccac
    11941 aagatcggat gagccagttt atcaatccgg gggtgccagc tgatccatgg agtgcagggt
    12001 ctgcaaaata tctcaagcac tgattgatct taggttttac aatagtgatg ttaccccagg
    12061 aacaatttgg ggaaggtcag aatcttgtag cctgtagctg catgactcct aaaccataat
    12121 ttcttttttg tttttttttt tttatttttg agacagggtc tcactctgtc acctaggctg
    12181 gagtgcagtg gtgcaatcac agctcactgc agcctcaacg tcgtaagctc aagcgatcct
    12241 cccacctcag cctgcctggt agctgagact acaagcgacg ccccagttaa tttttgtatt
    12301 tttggtagag gcagcgtttt gccgtgtggc cctggctggt ctcgaactcc tgggctcaag
    12361 tgatccagcc tcagcctccc aaagtgctgg gacaaccggg gccagtcact gcacctggcc
    12421 ctaaaccata atttctaatc ttttggctaa tttgttagtc ctacaaaggc agtctagtcc
    12481 ccaggcaaaa agggggtttg tttcgggaaa gggctgttac tgtctttgtt tcaaactata
    12541 aactaagttc ctcctaaact tagttcggcc tacacccagg aatgaacaag gagagcttgg
    12601 aggttagaag cacgatggaa ttggttaggt cagatctctt tcactgtctg agttataatt
    12661 ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag tcgctgcatt aatgaatcgg
    12721 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12781 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    12841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    12901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    12961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    13021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    13081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    13141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    13261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    13321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    13381 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    13441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    13501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    13561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    13621 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13681 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13741 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13801 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    13861 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    13921 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    13981 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    14041 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    14101 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    14161 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    14221 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    14281 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    14341 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    14401 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    14461 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    14521 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    14581 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    14641 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    14701 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    14761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    14821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    14881 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    14941 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    15001 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    15061 acgccagggg aggcagagat tgcagtaagc tgagatcgca gcactgcact ccagcctggg
    15121 cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat attccaatct
    15181 tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct tattccagaa
    15241 ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct tcacctgaaa
    15301 tgtgtgtata caaaatctag gccagtccag cagagcctaa aggtaaaaaa taaaataata
    15361 aaaaataaat aaaatctagc tcactccttc acatcaaaat ggagatacag ctgttagcat
    15421 taaataccaa ataacccatc ttgtcctcaa taattttaag cgcctctctc caccacatct
    15481 aactcctgtc aaaggcatgt gccccttccg ggcgctctgc tgtgctgcca accaactggc
    15541 atgtggactc tgcagggtcc ctaactgcca agccccacag tgtgccctga ggctgcccct
    15601 tccttctagc ggctgccccc actcggcttt gctttcccta gtttcagtta cttgcgttca
    15661 gccaaggtct gaaactaggt gcgcacagag cggtaagact gcgagagaaa gagaccagct
    15721 ttacaggggg tttatcacag tgcaccctga cagtcgtcag cctcacaggg ggtttatcac
    15781 attgcaccct gacagtcgtc agcctcacag ggggtttatc acagtgcacc cttacaatca
    15841 ttccatttga ttcacaattt ttttagtctc tactgtgcct aacttgtaag ttaaatttga
    15901 tcagaggtgt gttcccagag gggaaaacag tatatacagg gttcagtact atcgcatttc
    15961 aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt gatgactacc tcagttggat
    16021 ctccacaggt cacagtgaca caagataacc aagacacctc ccaaggctac cacaatgggc
    16081 cgccctccac gtgcacatgg ccggaggaac tgccatgtcg gaggtg
//
