LOCUS       sequence-379948-        9484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TFAP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Hagel KR, Arafeh R, Gang S, Arnoff TE, Larson RC, Doench JG, 
            Mathewson ND, Wucherpfennig KW, Maus MV, Hahn WC
  TITLE     Systematic interrogation of tumor cell resistance to CAR T cell 
            therapy in pancreatic cancer.
  JOURNAL   Cancer Res. 2022 Dec 22:CAN-22-2245. doi: 
            10.1158/0008-5472.CAN-22-2245.
  PUBMED    36548402
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2022 Dec 22:CAN-22-2245. doi: 10.1158/0008-5472.CAN-22-2245."
COMMENT     Sequence Label: pLX317 TFAP4
FEATURES             Location/Qualifiers
     source          1..9484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2238..2262)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2270..2311
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2422..3010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2475..2495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2893..2904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3082..3315
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3393..3514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3430..3449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3484..3503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3554..3689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3709..3728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3710..3728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3738..3755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3738..3754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3747..3769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3896..4351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3983..4002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4193..4214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4377..4481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4482..4550,4551..5342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4700..4719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5513..6101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6002..6021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6255..6272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6389..6410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6425..6430,6431..6448,6449..6455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6463..6479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6468..6490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6487..6503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6487..6503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6522..6542
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6524..6542
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6570..6796
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6797..6977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7024..7149
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7642..7875
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8060..8104
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8335..8545
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8357..8553
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8404..8539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8585..9187
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8585..8604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9081..9101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9230..9347
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggagta tttcatggtg cccactcaga aggtgccctc
     1261 tttgcaacat ttcaggaaaa cagagaaaga agtgatagga gggctctgta gccttgccaa
     1321 cattccacta acccccgaga ctcagcggga ccaggagcgg cggattcggc gggagatcgc
     1381 caacagcaac gagcggagac gcatgcagag catcaacgcg ggattccagt ccctcaagac
     1441 cctcatcccc cacacagacg gagagaagct cagcaaggca gccattctcc agcagacagc
     1501 cgagtacatc ttctccctgg agcaggagaa gaccaggctc ttgcagcaga acacacagct
     1561 caagcgcttc atccaggagc tgagcggctc gtcccccaag cgacggcggg cagaggacaa
     1621 ggacgaaggc ataggctccc cggacatctg ggaggacgag aaggcggagg acctgcggcg
     1681 ggagatgatt gagctgcggc agcagctgga caaggagcgc tcggtgcgca tgatgctgga
     1741 ggagcaggtg cgctcgctgg aggcccacat gtacccggaa aagctcaagg tgattgcgca
     1801 gcaggtgcag ctgcagcagc agcaggaaca ggtgaggctg ctgcaccagg agaagctgga
     1861 gcgggaacag cagcagctgc ggacccagct tctgccccct ccggccccca cccaccaccc
     1921 cacggtgatc gtgccagcac cgcctcctcc tccctcccac cacatcaatg tcgtcaccat
     1981 gggcccctcc tcggtcatca actctgtttc cacatcccgg caaaatctgg acaccatcgt
     2041 gcaggcaatc cagcacatcg agggcaccca ggaaaagcag gagctggagg aggagcagcg
     2101 gcgagctgtc atcgtgaagc ctgtccgcag ctgcccggag gcccccacct ctgacaccgc
     2161 ctccgactcc gaggcctcag acagtgacgc catggaccag agccgggagg agccgtcggg
     2221 ggacggggag cttccctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     2281 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     2341 tatttcgatt tcttggcttt atatatcttg tggaaaggac gacaaacctt tcccaagcct
     2401 ccaatcacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2461 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2521 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2581 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2641 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2701 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2761 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2821 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2881 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2941 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3001 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3061 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3121 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3181 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3241 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3301 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3361 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     3541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3661 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     3721 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3781 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3841 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4321 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5341 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5401 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5461 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5641 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5881 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6181 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6241 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6361 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6421 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6481 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     6541 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     6601 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     6661 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     6721 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6781 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6961 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7021 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7081 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7141 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7201 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7261 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7321 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7381 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7441 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     7501 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7561 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7621 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7681 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7741 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7801 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7861 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7921 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7981 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8041 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8101 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8161 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8221 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     8281 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8341 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8401 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8461 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8521 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     8581 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8641 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8701 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8761 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     8821 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8881 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8941 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9001 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9061 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9121 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9181 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9241 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9301 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9361 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     9421 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     9481 aatt
//
